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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Journal of Agromedicine and Medical Sciences Jurnal Informasi Kesehatan Indonesia (JIKI) Journal of Nursing Science Update
Hidayat Sujuti
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Agriculture, Biological Sciences & Forestry Environmental Science Health Professions Nursing Public Health

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 1 
The Effect of Hyaluronic Acid on MMP-9 Expression in Trabecular Meshwork Cell Culture of Patient with Primary Open Angle Glaucoma Cicih Komariah; Ma’sum Effendi; Hidayat Sujuti
Journal of Agromedicine and Medical Sciences Vol 3 No 1 (2017)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v3i1.4096

Abstract

In primary open angle glaucoma, decrease of hyaluronic acid level is related to extracellular matrix deposition of trabecular meshwork. Extracellular matrix deposition will increase humor aqueous outflow resistance which in turn increase the intraocular pressure. MMP-9 as one of matrix metalloproteinase (MMP) family, has a high potency to degrade the extracellular matrix. The expression of MMP-9 can be activated by hyaluronic acid through its binding with CD44 as hyaluronic main receptor. The aim of this research is  to determine the effect of hyaluronic acid on MMP-9 expression in trabecular meshwork cell culture of patient with primary open angle glaucoma by immunocytochemical method. Trabecular meshwork cell culture were exposed to hyaluronic acid at four different concentration (0mg/ml, 1 mg/mL, 3 mg/mL, 6 mg/mL). After 24 hours incubated, we find a significant difference of MMP-9 expression between control group and group with hyaluronic acid. There was significant correlation between hyaluronic acid and MMP-9 expression. Hyaluronic acid exposure will increase MMP-9 expression in trabecular meshwork cell culture. This finding suggest that hyaluronic acid can influence the resistance of humor aqueous outflow in trabecular meshwork by up-regulating MMP-9 expression, and therefore will increase extracellular matrix degradation.Key words : hyaluronic acid, primary open angle glaucoma, MMP-9
Pengaruh Extra Virgin Olive Oil Terhadap Folikulogenesis dan Kadar Malondialdehyde Ovarium Pada Tikus yang dipapar Rhodamin B Huda Rohmawati; Hidayat Sujuti; Pande Made Dwijayasa; Hendy Setyo Yudanto
Jurnal Informasi Kesehatan Indonesia (JIKI) Vol 4 No 2 (2018): Jurnal Informasi Kesehatan Indonesia
Publisher : Politeknik Kesehatan Kemenkes Malang (State Health Polytechnic of Malang)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31290/jiki.v(4)i(2)y(2018).page:120-127

Abstract

Rhodamin B merupakan bahan pewarna tekstil disalahgunakan sebagai pewarna makanan. Rhodamin B bersifat toksik penyebab stres oksidatif, dan meningkatkan kadar MDA. Peningkatan kadar MDA menyebabkan gangguan folikulogenesis sehingga folikel tidak berkembang dan atresia. Hal tersebut berkontribusi pada kejadian infertil. Paparan rhodamin B berpengaruh pada folikulogenesis dan kadar MDA ovarium. Tujuan dari penelitian ini adalah membuktikan pengaruh EVOO pada Folikulogenesis dan kadar Malondialdehyde ovarium tikus betina galur wistar yang dipapar rhodamin B. Metode penelitian menggunakan True Experimental Post Test Only Control Group menggunakan 25 ekor tikus dibagi menjadi 2 kelompok kontrol dan 3 kelompok perlakuan dengan pemberian EVOO peroral dosis 1,5 ml/KgBB, 3 ml/KgBB, 4,5 ml/KgBB serta dipapar rhodamin B 18 mg/KgBB selama 36 hari. Kemudian dilakukan pengambilan ovarium untuk menghitung jumlah folikel dengan pewarnaan HE dan pengukuran kadar MDA dengan Spectrofotometri. Pemberian EVOO terhadap jumlah folikel primer, sekunder dan kadar MDA menunjukkan korelasi yang bermakna (p=0,000<α), dengan koefisien korelasi folikel primer 0,735, folikel sekunder 0,699, menunjukkan ada hubungan yang positif dan kuat. Pada kadar MDA didapatkan -0,808 sehingga ada hubungan yang negatif dan kuat. Kesimpulan: Pemberian EVOO dengan dosis 1,5 ml/KgBB, 3 ml/KgBB, 4,5 ml/KgBB berpengaruh terhadap folikulogenesis dan penurunan kadar MDA ovarium pada tikus betina yang dipapar rhodamin B.
HUBUNGAN LUAS INFARK MIOKARD (BERDASAR SKOR SELVESTER) DENGAN RESPON NYERI DADA PADA PASIEN SINDROM KORONER AKUT (SKA) DI RSD Dr. SOEBANDI JEMBER Cipto Susilo; Hidayat Sujuti; Titin Andri Wihastuti
Journal of Nursing Science Update (JNSU) Vol. 1 No. 2 (2013)
Publisher : Department of Nursing, Faculty of Health Sciencce, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.695 KB)

Abstract

Kegawatan penyakit jantung pada Sindrom Koroner Akut (SKA) bersifat progresif yang mencakup angina tidak stabil, ST-elevation myocardial infarction (STEMI) dan non-ST elevation myocardial infarction (NSTEMI). Diagnosis SKA berdasar 3 komponen yang harus ditemukan yakni: gejala iskemia sakit dada, perubahan elektokardiografi (EKG) dan peningkatan enzim jantung. Penggunaan Selvester skore dapat menentukan luas dan lokasi infark miokard karena dapat menilai perubahan depolarisasi ventrikel. Penelitian bertujuan mengetahui adanya hubungan antara  luas infark miokard (berdasar skor Selvester) dengan respon nyeri dada pada pasien SKA. Penelitian ini termasuk analitik observasional dengan desain Cross Sectional dan teknik pengambilan sampel consecutive sampling dengan sampel 20 responden. Hasil analisa menggunakan uji statistik Regresi Logistik menunjukkan luas infark miokard memiliki pengaruh signifikan pada model jenis respon nyeri sedang dan berat dengan nilai p: 0.045 untuk nyeri sedang dan nilai p: 0.019 untuk nyeri berat. Hasil penelitian tersebut dapat disimpulkan bahwa semakin luas infark miokard akan semakin berat nyeri dada yang dirasakan pasien dengan SKA.   Kata Kunci: Luas infark, skor selvester, nyeri dada, SKA
The effect of Nigella Sativa extract on Alpha-ketoglutarate activity and histopathologic changes on Rat liver induced by Monosodium glutamate Ala Shukri Eshami; Karyono mitaroem; Hidayat Sujuti; Abdussalam Ashour
Journal of Tropical Life Science Vol. 5 No. 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.03

Abstract

         Monosodium glutamate (MSG) is a commonly used food additive and found in most soups, fish and processed meat. The use of MSG in food is growing. Irrational fear had increased in the last few years due to the adverse reactions and toxicity of MSG, which effect on the liver.  Nigella sativa is used as traditional medicine for the treatment of many diseases, it has been extensively investigated in recent years, traditional medicine for the treatment of many disease due to its notable pharmacological properties. NS can inhibit oxidative stress. The present study was undertaken to investigate different Doses of Nigella sativa on alpha KGDH activity and liver histology of MSG induced rat. The animals (n=30) Were grouped  A as (control), B treated with MSG 1g/kg, C MSG treated with NS 0.01g/kg, D MSG treated with NS 0.02g/kg, E MSG treated with NS 0.04g/kg and F treated with NS 0.02g/kg then we investigated the effect alpha KGDH activity by ELISA method and liver histopathology by light microscope.  alpha KGDH activity were significantly increased in treatment MSG compared with treatment negative control, MSG + NS 0.1g/kg, MSG + NS 0.02g/kg, MSG + NS 0.04g/kg, and NS 0.02g/kg. The histological changes in group B and C showed disturbed liver architecture, hemorrhage in the central veins, areas of necrosis, vacuolation and increased inflammatory cells infiltration while group D showed central vein is normal and vacuolation of cytoplasm, group E showed more normal liver architecture as shown less vacuolation in cytoplasm, central vein is normal, and group F showed no pathologic changes.These finding showed that administration of MSG increase alpha KGDH and induced damage in liver tissue. Nigella sativa extract can reduce alpha KGDH and prevent liver damage induced by MSG.Keywords :  Monosodium glutamate, Alpha-ketoglutarate Dehydrogenases, Nigella sativa and liver damage.
Rho- Kinase Inhibitor Y-27632 Decreases the Thickness of Trabecular Meshwork in Juvenile Rats Mod-el Injected with Sodium Hyaluronate Wino Vrieda Vierlia; Lely Retno Wulandari; Hidayat Sujuti; Ma'sum Effendi
Journal of Tropical Life Science Vol. 8 No. 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.13

Abstract

Most glaucoma drugs lower the intraocular pressure (IOP) by decreasing the aqueous humor production and increasing the outflow through uveoscleral pathway. None of these drugs work mainly on increasing outflow through the trabecular pathway. Consequently, the experiment to develop glaucoma drugs directly target at the trabecular outflow pathway is highly required. The purpose of this study is to reveal the effect of rho-kinase inhibitor Y-27632 on the thickness of trabecular meshwork in juvenile rats model injected with sodium hyalu-ronate.  This study was an experimental study with posttest only control group design. Twenty-four rats were included in this study. Each eye of the rat would be considered as one sample. Samples were divided into 6 groups, negative control group, positive control I group with intracameral sodium hyaluronate injection, posi-tive control II group with topical Y-27632 10 mM, and three experimental groups with intracameral injection of sodium hyaluronate and Y-27632 10-1 mM, 1 mM, and 10 mM respectively. After the procedures all rats were sacrificed and enucleated. Trabecular meshwork tissue was stained with Hematoxilene-Eosin and evalu-ated under 400× microscopic magnification. Quantitative measurements were taken using computerized image analysis with dot slide program.  There were significant statistic differences among the positive control I group and the experimental groups (p-value < 0.05) as well as the positive control II group and the experi-mental groups (p-value < 0.05). The highest mean of decreasing trabecular meshwork thickness was noted in the group given by sodium hyaluronate and Y-27632 10 mM with value of 118.42 µm. There was decreasing thickness of trabecular meshwork due to the effect of rho- kinase inhibitor Y-27632 in juvenile rats injected with sodium hyaluronate.
α-SMA Expression Increased Over Cell Passages and Decreased by Exogenous TGF-β1, In Vitro Studies on Myofibroblast Derived from Orbital Socket Contracture Debby Shintiya Dewi; Elsa Safira Chairinnisa; Hidayat Sujuti; Diana Lirawati; Tinny Endang Hernowati
Journal of Tropical Life Science Vol. 8 No. 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.15

Abstract

α-smooth muscle actin (α-SMA), a marker of myofibroblast, induces cytoskeleton reorganization, increases contractility and stimulates cell migration in TGF-β1 induced stress fibers. The aims of the present study were to determine the level of α-SMA expression and morphological cell changes in different passages of myofi-broblasts with varied TGF-β1 concentrations. Myofibroblast cell cultures were derived from fibrotic tissues of fourth degree socket contracture. The α-SMA expression level was measured in myofibroblast cultures pas-sage I, II, and III with and without 10 ng/mL TGF-β1, and in passage III with 2.5; 5; 10; and 20 ng/mL TGF-β1. Results: The levels of α-SMA expression level in passage I to III were I 31.42 ± 3.4; 40.34 ± 8.14 and 56.37 ± 7.57, respectively. Addition of 10 ng/mL TGF-β1 into passage I-III myofibroblast cultures resulted in α-SMA expression level of 31.24 ± 2.93; 36.81 ± 6.09; and 14.29 ± 2.72, respectively. Myoblasts passage III showed the lowest α-SMA expression level following exposure to TGF-β1 10 ng/mL (22.37 ± 12.86) and highest without TGF-β1 (48.34 ± 13.36), however no morphological changes detected. α-SMA expression level increased with cell passages, decreases with addition of TGF-β1 while not affecting morphology of myofibroblast derived from the orbital socket contracture.
PENGARUH EXTRA VIRGIN OLIVE OIL TERHADAP FOLIKULOGENESIS DAN KADAR MALONDIALDEHYDE OVARIUM PADA TIKUS YANG DIPAPAR RHODAMIN B Huda Rohmawati; Hidayat Sujuti; Pande Made Dwijayasa; Hendy Setyo Yudanto
Jurnal Informasi Kesehatan Indonesia (JIKI) Vol 4 No 2 (2018): Jurnal Informasi Kesehatan Indonesia
Publisher : Politeknik Kesehatan Kemenkes Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31290/jiki.v4i2.356

Abstract

Rhodamin B merupakan bahan pewarna tekstil disalahgunakan sebagai pewarna makanan. Rhodamin B bersifat toksik penyebab stres oksidatif, dan meningkatkan kadar MDA. Peningkatan kadar MDA menyebabkan gangguan folikulogenesis sehingga folikel tidak berkembang dan atresia. Hal tersebut berkontribusi pada kejadian infertil. Paparan rhodamin B berpengaruh pada folikulogenesis dan kadar MDA ovarium. Tujuan dari penelitian ini adalah membuktikan pengaruh EVOO pada Folikulogenesis dan kadar Malondialdehyde ovarium tikus betina galur wistar yang dipapar rhodamin B. Metode penelitian menggunakan True Experimental Post Test Only Control Group menggunakan 25 ekor tikus dibagi menjadi 2 kelompok kontrol dan 3 kelompok perlakuan dengan pemberian EVOO peroral dosis 1,5 ml/KgBB, 3 ml/KgBB, 4,5 ml/KgBB serta dipapar rhodamin B 18 mg/KgBB selama 36 hari. Kemudian dilakukan pengambilan ovarium untuk menghitung jumlah folikel dengan pewarnaan HE dan pengukuran kadar MDA dengan Spectrofotometri. Pemberian EVOO terhadap jumlah folikel primer, sekunder dan kadar MDA menunjukkan korelasi yang bermakna (p=0,000<α), dengan koefisien korelasi folikel primer 0,735, folikel sekunder 0,699, menunjukkan ada hubungan yang positif dan kuat. Pada kadar MDA didapatkan -0,808 sehingga ada hubungan yang negatif dan kuat. Kesimpulan: Pemberian EVOO dengan dosis 1,5 ml/KgBB, 3 ml/KgBB, 4,5 ml/KgBB berpengaruh terhadap folikulogenesis dan penurunan kadar MDA ovarium pada tikus betina yang dipapar rhodamin B.
Co-Authors Abdussalam Ashour Ala Shukri Eshami Cicih Komariah Cipto Susilo Debby Shintiya Dewi Diana Lirawati Elsa Safira Chairinnisa Hendy Setyo Yudanto Huda Rohmawati Karyono mitaroem Lely Retno W Ma'sum Effendi Ma’sum Effendi Pande Made Dwijayasa Tinny Endang Hernowati Titin Andri Wihastuti Wino Vrieda Vierlia