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All Journal journal of Basic Medical Veterinary Berkala Penelitian Hayati
Erma Safitri
Fakultas Kedokteran Hewan Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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ANTI-PROLAKTIN SEBAGAI PENGHAMBAT PROSES MOULTING Husni Anwar; Erma Safitri
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/455

Abstract

A experimental study had been done using anti-prolactin as moulting process inhibitor and toknow the affect of anti-prolactin on the time of egg laying recovery of laying bird. The objective of this study was to prove that, moulting phase can be stopped with pasif imunisation antiprolactin intra muscular. The stages of this study as follow: used anti-prolactin from the third stage of study with the dose of 50 mg/ml (P1), 100 mg/ml (P2), and 200 mg/ml (P3) and 0.5 ml PBS (as control). In this stage, moulting was ceased respectively in day 4.8 ± 1.033 (P1); 4.6 ± 0.843 (P2); 4.68 ± 0.516 (P3) and 61.9 ± 2.079 (control). Statistical test using one way Anova revealed significant difference (p < 0.01) between control and treatment groups, and BNT 5% test showed that the fastest moulting inhibition was found in P3 group (200 mg/ml), which was not significantly different (p < 0.05) with P1 (50 mg/ml) and P2 (100 mg/ml). The time of egg laying recovery was respectively at 9.3 ± 0.675 (PI); 7.4 ± 0.843 (P2); 3.3 ± 0.823 (P3) and 18.4 ± 1.174 (control). Statistical test using one way Anova test showed significant difference (p < 0.01) between control and treatment groups, and BNT 5% test indicated that the shortest time of egg laying recovery was at group P3 (200 mg/ml), which was significantly different (p < 0.05) from Pl (50 mg/ml); P2 (100 mg/ml); and control (PBS 0.5 ml). Conclusively, anti prolactin (50 mg/ml, 100 mg/ml and 200 mg/ml) to inhibit moulting process and to affect the time of egg laying recovery.
METODE PEMBUATAN ANTIPROLAKTIN PADA HEWAN COBA KAMBING LOKAL SEBAGAI PENGHAMBAT PROSES RONTOK BULU PADA AYAM ARAB PETELUR Erma Safitri
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/458

Abstract

Anti-prolactin has a specific activity against prolactin. It neutralizes prolactin action in circulation. The effect of such neutralization is the inhibition of feather fall off process, so that hens may be able to produce eggs again. Anti-prolactin can be produced by injecting prolactin isolate from blood serum of arabic hens in feather fall off-phase into local goat. Prolactin isolate was immunized to local goat to produce anti-prolactin. Six local goats were divided into 2 groups. The first group comprised 1 goat immunized with PBS, and the second one was immunized with prolactin isolate in CFA and subjected to booster with prolactin isolate in IFA twice. The formation of anti-prolactin and the highest titer was detected using indirect ELISA. The results of this study showed that (1) Anti-prolactin could be produced in goat from the prolactin isolate of feather fall off-phase arabic hens blood serum; (2). The first emergence of Antiprolactin was at the first bleeding after immunization of prolactin isolate in CFA and first booster in IFA. The highest titer was found at eleventh bleeding after the third booster with prolactin isolate in IFA.
PENGARUH PEMBERIAN PROPOLIS LEBAH TERHADAP GAMBARAN HISTOPATOLOGI HEPAR MENCIT (Mus musculus) BETINA YANG DIPAPAR LOGAM BERAT PB ASETAT [Pb(C2H3O2)2] Sartika Dewi Rachmani; Erma Safitri; Roesno Darsono; Eka Pramyrtha Hestianah; Hani Plumeriastuti; Kuncoro Puguh Santoso
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19988

Abstract

Lead acetate in the hepatobiliary system may cause peroxidation catalysis of unsaturated fatty acids, reduce nitrogenoxide and increase hydroxyl radical. Lead acetate produces oxidative stress characterized by free radical formation and inhibits lipid peroxidation. Giving antioxidants can neutralize free radicals from the detrimental effects that arise on the process or excess oxidation reactions. The purpose of this research was to find out how the effect of bee propolis on histopathologic images of hepatic mice (Mus musculus) of females exposed to lead acetate [Pb(C2H3O2)2]. The subjects were 25 mice (Mus musculus) mushulus of BALB/C strain with average weight 25-30 gram and 8 weeks old, divided into 5 treatment groups, each consisting of 5 heads per group. The K- group was given a Tween 80 solution at a doses of 0.5 mg/kgBW for 20 consecutive days. The K+ group, which was given only lead acetate at a doses of 10 mg/kgBW orally for 10 days. P1, P2, and P3 were given 10 mg/kgBW lead acetate solution orally for 10 days. The following 10 days were given bee propolis with doses of P1 200 mg/kgBW, P2 400 mg/kgBW, and P3 800 mg/kgBW. On the 21th days the mice were dissected, to observe the extent of the damage. All data were performed using a statistical test with Kruskal Wallis test and if there was a marked difference between treatment groups (p<0.05), then the Mann-Whitney test was followed. The results obtained that bee propolis can repair hepatic cell damage in mice (Mus musculus) of females exposed to lead acetate. Increased dose of bee propolis is ineffective in repairing hepatic cell damage in mice (Mus musculus) of females exposed to lead acetate.
Co-Authors Eka Pramyrtha Hestianah Hani Plumeriastuti Husni Anwar Kuncoro Puguh Santoso Roesno Darsono Sartika Dewi Rachmani