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All Journal Jurnal Natur Indonesia Jurnal Akademika Biologi Medula International Journal of Science, Engineering, and Sustainable Technology (IJSESTech) JOURNAL ONLINE OF PHYSICS ANNALES BOGORIENSES Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017
Sapto Raharjo
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Immunology & microbiology Medicine & Pharmacology Nursing Physics Public Health

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Upgrading Traditional Techniques of Food Fermentation: Kabuto-Making Susilowati, Prima Endang; ., Sanatang; Safitri, Meilani; Raharjo, Sapto
International Journal of Science, Engineering, and Sustainable Technology (IJSESTech) Vol 1, No 1 (2014)
Publisher : International Journal of Science, Engineering, and Sustainable Technology (IJSESTech)

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Abstract

The capacity to preserve food is directly related to the level of technological development. The slow progress in upgrading traditional food processing and preservation techniques in Sulawesi Tenggara contributes to food and nutrition insecurity in the sub-region. Simple, low-cost, traditional food processing techniques are the bedrock of small-scale food processing enterprises that are crucial to rural development in Sulawesi Tenggara. While a lot still needs to be done, some successes have been achieved in upgrading traditional sulawesi Tenggara food processing technologies including the mechanization of kabuto (fermented cassava meal) processing.
HUBUNGAN POLIMORFISME GEN ANGIOTENSINOGEN M235T DENGAN HIPERTENSI ESENSIAL PADA ETNIS SULAWESI TENGGARA Raharjo, Sapto; Chahyadi, Agus; Fadhilah, Aulia; Tien, Tien; Kholidha, Andi Noor; Subijakto, Sjarif
Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017 Vol 1, No 1 (2017): Seminar Nasional Riset Kuantitatif Terapan 2017
Publisher : Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017

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Abstract

Patogenesis penyakit hipertensi esensial dipengaruhi oleh faktor genetik dan lingkungan. Gen renin-angiotensin-aldosterone system (RAAS) telah dipelajari secara ekstensif untuk perannya dalam mengontrol tekanan darah. Ada berbagai gen yang mengkode komponen di RAAS yang terlibat dalam pengembangan hipertensi. Polimorfisme gen ini sangat diyakini terkait dengan prevalensi hipertensi. Studi Polimorfisme gen angiotensinogen 235M/T menunjukkan bahwa alel 235T pada orang Asia meningkatkan risiko hipertensi dibandingkan pada orang kulit putih. Penelitian ini  mengevaluasi frekuensi polimorfisme AGT (M235T) dalam kaitannya dengan hipertensi esensial pada etnis di Sulawesi Tenggara. Desain penelitian ini adalah desain cross sectional dengan varian angiotensinogen M235T terhadap Hipertensi Esensial sebagai variabel independen. Penelitian ini menggunakan pendekatan biologi molekuler untuk menginterpretasikan hasil elektroforesis DNA dari sampel yang sebelumnya telah diperiksa menggunakan teknik PCR dan RFLP. Hasil penelitian ini menunjukkan bahwa polimorfisme gen angiotensinogen M235T tidak memiliki hubungan dengan kejadian hipertensi esensial di Etnis Sulawesi Tenggara. Uji chi-square menunjukkan bahwa nilai p lebih besar dari nilai alpha (α) (nilai p 0,275> α (0,005)). Hal ini menunjukkan bahwa polimorfisme gen M235T bukan faktor risiko pada pengembangan penyakit jantung hipertensi. Kesimpulan dari penelitian ini adalah polimorfisme gen angiotensinogen M235T tidak memiliki hubungan dengan kejadian hipertensi esensial di etnis Sulawesi Tenggara.Kata kunci— Hipertensi Esensial, Gen Angiotensinogen, Polimorfisme Gen.
PEMANFAATAN ENZIM PAPAIN DALAM PEMBUATAN PEPTIDA A. sativum SEBAGAI INHIBITOR ANGIOTENSIN I-CONVERTING ENZYME Raharjo, Sapto; Sulviyana, Nur; Hamra, Yusuf; Cahyadi, Agus
Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017 Vol 1, No 1 (2017): Seminar Nasional Riset Kuantitatif Terapan 2017
Publisher : Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017

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Abstract

Bawang putih (Allium sativum) merupakan salah satu herbal yang digunakan sebagai antihipertensi. Penelitian ini bertujuan meningkatkan kadar peptida dari ekstrak A. sativum menggunakan enzim papain, sehingga mampu meningkatkan penghambatan/inhibisi terhadap aktivitas Angiotensin-I Converting Enzyme (ACE). Hasil isolasi protein A. sativum memperlihatkan kadar asam amino dan total protein  masing-masing 217,57±6,67 µg L-Tyr/g A. sativum dan 686,05±4,29 µg protein/g A. sativum. Hidrolisis dengan papain mampu menurunkan total protein A. sativum dari 333,12±15,46 µg/mL menjadi 80,09±0,90 µg/mL, atau turun sekitar 4 kali. Hasil tersebut berbanding lurus dengan kadar asam amino yang meningkat sekitar 16 kali, dari 18,26±2,23 µg/mL menjadi 291,60±8,16 µg/mL. Uji penghambatan aktivitas ACE menunjukkan ekstrak peptida A. sativum memiliki daya hambat yang lebih tinggi dibandingkan dengan ekstrak protein A. sativum yang tidak dihidrolisis. Nilai IC50 diperoleh pada konsentrasi 457,087 ppm.Kata kunci— Allium sativum, Antihipertensi, Angiotensin –I converting enzyme.
Morphology and molecular characterization of newly isolated microalgae strain Chlorella volutis LIPI13-WKT066 from Wakatobi Islands and its potential use Rahman, Delicia Yunita; Praharyawan, Swastika; Raharjo, Sapto; Fadiyah, Farizul; Susilaningsih, Dwi
ANNALES BOGORIENSES Vol 23, No 1 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2019.v23.n1.13-19

Abstract

Morphology and molecular characterization of microalgae isolated from Wakatobi Marine National Park was conducted. An understanding of the characteristics of morphology, molecular, as well as metabolites profile of the microalgae species is potentially useful for its future application. The primary aim of this study was to isolate, identify and characterize the microalgae strain isolated from Wakatobi Marine National Park labeled as LIPI13-WKT066 with the emphasis on the evaluation of amino acid content as a basis for its health application. Morphological observation under the microscope and molecular identification suggested that the microalgae strain of LIPI13-WKT066 belong to the strain under species of Chlorella volutis. Metabolite characterization of the microalgae strain showed that the content of protein (11.9%), lipid (12.4%) and carbohydrate (4.7%) was in the regular range. Further analysis of its amino acid content revealed the potency of the microalgae strain to be used as antihypertensive agent.
Produksi Xilanase dari Isolat Sumber Air Panas Sonai, Sulawesi Tenggara, menggunakan Limbah Pertanian Susilowati, Prima Endang; Raharjo, Sapto; Kurniawati, Desi; Rahim, Rahmawati; Sumarlin, Sumarlin; Ardiansyah, Ardiansyah
Jurnal Natur Indonesia Vol 14, No 3 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.469 KB) | DOI: 10.31258/jnat.14.3.199-204

Abstract

Xylanase is the enzyme with prospec for hydrolysis hemicellulases. Many industry use xylanase thermostable. This processes require enzymes which are operationally stable at high temperature thus allowing e.g. easy mixing, better substrate solubility, high mass transfer rate, and lower risk of contamination. Thermophiles have often been proposed as sources of industrially relevant thermostable enzymes. Thermophilic bacteria, live at hot-springs, are can produced thermostable enzymes. In this work, we studied the bacteria strains isolated from water collected in the Sonai hot-springs of Southeast Sulawesi region and condition production enzyme. Screaning bacteria xylanolitic use semi-quantitative detection at agar plate with xilan, and quantitative detection activity enzyme. Result experinces in the 28 isolates screened, isolat IIA-3 (Pseudomonas sp.) showed the highest xylanase production. Organism efficiently used 2% rice straw as substrates. Pseudomonas sp. was used to hydrolyses rice straw at 50oC, agitation 150 rpm and pH 9.
Uji Aktivitas Antibakteri Ekstrak Metanol Rumput Laut (Kappaphycus alvarezii) pada Berbagai Tingkat Konsentrasi terhadap Pertumbuhan Bakteri Streptococcus mutans Agus Saifuddin; Sapto Raharjo; Amiruddin Eso
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 3, No 1 (2015)
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (177.821 KB) | DOI: 10.46496/medula.v3i1.2541

Abstract

Red algae Kappaphycus alvarezii contain flavonoids compound which has activity as an anti bacterial. This study aims to determine the difference of inhibition zone of the growth of Streptococcus mutans bacteria by seaweed methanol extract (K. alvarezii) at each concentrations. This research was conducted by post–test only design (one-shot case study) with a variable treatment of the seaweed methanol extract (K. alvarezii) against S. mutans. Extracts were then divided into 7 concentration of 5%, 25%, 50%, 65%, 75%, 85%, and 95%. Data were analyzed to determine the difference of inhibition zone seen from the ANOVA test (analysis of varience) followed by a post hoc test. The results of the bivariate analysis showed there were no difference of inhibition zone of the growth of S. mutans bacteria by seaweed methanol extract of K. alvarezii at the concentration of 65%, 75%, 85%, and 95% (p = 0,143). This research concluded that there were no defference of inhibition zone of the growth of S. mutans bacteria by seaweed methanol extract of K. alvarezii.Keywords : Kappaphycus alvarezii, Streptococcus mutans
Uji Aktivitas Antioksidan Mikroalga Air Tawar Rio Kristian; Sapto Raharjo; Sulastrianah Sulastrianah
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 3, No 1 (2015)
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (378.1 KB) | DOI: 10.46496/medula.v3i1.2537

Abstract

Cell or tissue damage due to oxidative stress can be slowed by giving antioxidants. Antioxidants are vital substances that may help protect the body against free radicals by neutralizing or mitigate negative impacts. The human body is naturally equipped antioxidant defenses. but these antioxidants can not completely prevent cell damage. Body still requires antioxidants from the outside. The purpose of this study was to determine the antioxidant potential of microalgae species in freshwater.This research is a descriptive study of the identification of species of freshwater microalgae samples taken with a microscope and testing of extracts from freshwater microalgae species of antioxidant activity using DPPH method. Microalgae extract as much 2,25ml then homogenized with DPPH as 0,75ml in containers with a volume of 3ml. The containers are then incubated for 30 minutes in a dark room. Furthermore, the container is inserted into the spectrophotometer to read absorbance at 519nm wavelength. Results absorbance at the input curves to obtain IC50 values.The results that have identified three species of freshwater microalgae namely Navicula sp., Oscillatoria sp., And Carteria sp. From all three species showed strong inhibition against DPPH with IC50 values sequentially 41.304 ppm, 23.401 ppm and 51.433 ppm. The conclusions of this study are species of freshwater microalgae are obtained, namely Navicula sp., Oscilatoria sp., And Carteria sp. has potential antioxidant activityKeywords: Microalgae, antioxidant, DPPH
PENGARUH BEBERAPA KONSENTRASI MOLASE TERHADAP KUALITAS AIR PADA AKUARIUM IKAN BANDENG Meiza Putri; Fuad Muhammad; Jafron Wasiq Hidayat; Sapto Raharjo
Jurnal Akademika Biologi Vol. 5 No. 2 April 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

In 2013, the national aquaculture production is targeted to support the 14.8 million tons. One kultivan cultivated namely milkfish (Chanos chanos). Artificial feeding may lead to excessive tercernanya can not feed the fish, so that the necessary alternative fermented feed for fish growth. This study aims to assess the effect of several concentrations of molasses to the growth of fish. Research conducted in last August until October 2015. The method used is feeding pellets as much as 1 gram. Giving fermented feed ( molasses ) of 0 % , 2 % , 5 % and 7 % with three replications at 08.00 am today and measurement media quality water on the logs do every single week. Measurements were made using a DO meter, pH meter, thermometer and measuring Ammonia No3, No2, Fosfart, alkalinity and Tom were analyzed at the Laboratory of Physics - Chemistry BBPBAP. Analysis of the data used is using ANOVA test and Duncan Multile Range Test (DMRT). Results showed the effect of several concentrations Award (molasses) containing the bacteria Bacillus sp. and the most optimal pellet feed to the media quality of water present in a concentration of 2%. This is due to the lower molasses, can be absorbed by the fish so it is not the piling at the base of the aquarium. While the concentration of 5% and 7% are less than optimal. It thus allegedly because the feed is too much carbohydrates, can increase the potential for growth of pathogenic bacteria in the bottom of the aquarium
PENGARUH TEMPERATUR AKTIVASI TERHADAP KUALITAS BRIKET ARANG AKTIF SEKAM PADI La Ode Rusman; Lina Lestari; Sapto Raharjo; Ida Usman; Desi Chrismiwahdani
JOURNAL ONLINE OF PHYSICS Vol. 8 No. 3 (2023): JOP (Journal Online of Physics) Vol 8 No 3
Publisher : Prodi Fisika FST UNJA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jop.v8i3.23846

Abstract

Kajian tentang analisis kualitas briket arang sekam padi yang diaktivasi dengan tanur telah berhasil dilakukan. Penelitian ini merupakan penelitian eksperimen yang bertujuan untuk menentukan pengaruh variasi suhu aktivasi terhadap kualitas briket arang aktif sekam padi. Kualitas briket arang dapat ditentukan dari kerapatan, kadar air, kadar abu, volatile matter, fixed carbon, nilai kalor dan uji pembakaran untuk mengamati waktu sulut dan laju nyala. Penelitian ini dilakukan melalui beberapa tahap yaitu penyiapan bahan baku, karbonisasi, penggerusan dan pengayakan, pencampuran bahan perekat, pencetakan, pengeringan dan penentuan mutu briket. Briket arang aktif sekam padi dibuat dengan menggunakan sagu sebagai perekat dangan perbandingan massa 9:1. Serbuk arang sekam padi diayak dengan ayakan berukuran 70 - 80 mesh. Sampel dicetak menggunakantekanan 9,5x106 N/m2 dan diaktivasi dengan tanur pada suhu 450°C, 475°C, dan 500°C selama 4 menit. Hasil penelitian menunjukkan bahwa kerapatan yang diperoleh berkisar 0,606 - 0,640 g/cm3, kadar air berkisar 4,57-5,29%, kadar abu berkisar antara 4,89 - 6,57%, volatile matter berkisar antara 70,15 - 75,35%, nilai kalor berkisar 4923,58 - 5131,84 kal/g. Arang yang diaktivasi pada suhu 500°C menunjukkan kualitas baik dengan waktu sulut 5,5 menit, laju pembakaran 0,08 g/menit, temperatur maksimum 482,5°C dan lama pembakaran selama 35 menit.
Co-Authors Agus Saifuddin Cahyadi, Agus Chahyadi, Agus Desi Chrismiwahdani Desi Kurniawati DWI SUSILANINGSIH Eso, Amiruddin Fadhilah, Aulia Fadiyah, Farizul Fuad Muhammad Holyness Nurdin Singadimedja Ida Usman Jafron Wasiq Hidayat Kholidha, Andi Noor La Ode Rusman Lina Lestari Meilani Safitri Meiza Putri Prima Endang Susilowati Rahman, Delicia Yunita Rahmawati Rahim Rio Kristian Sanatang ., Sanatang Sjarif Subijakto, Sjarif Sulastrianah Sulastrianah, Sulastrianah Sulviyana, Nur Sumarlin Sumarlin Swastika Praharyawan, Swastika Tien Tien Yusuf Hamra, Yusuf