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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Indonesian Journal of Electronics and Instrumentation Systems Jurnal Ilmu Dasar Microbiology Indonesia BERKALA SAINSTEK Biogenesis: Jurnal Ilmiah Biologi Global Medical and Health Communication UNEJ e-Proceeding Universa Medicina Jurnal Bioteknologi & Biosains Indonesia (JBBI) BIOEDUKASI Warta Pengabdian Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi
Kartika Senjarini
Biology Department, Faculty Of Mathematic And Natural Sciences, University Of Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Dentistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Nursing Physics Public Health Veterinary

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Journal : BIOEDUKASI

 1 
MORPHOLOGICAL CHARACTERISTIC DIFFERENCE BETWEEN MOSQUITOES VECTOR FOR MALARIA AND DENGUE FEVER Syubbanul - Wathon; Kartika Senjarini; Rike Oktarianti; Ratis Nour Sholichah; Ahmad Tosin
BIOEDUKASI Vol 18 No 2 (2020)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v18i2.18890

Abstract

Malaria and Dengue Haemorrhagic Fever (DHF) are two major tropical diseases in East Java. The pathogens of these diseases are transmitted to human hosts via haematophagy by vector mosquitoes. Anopheles spp. specifically transmits Plasmodium parasites which cause Malaria, while Aedes spp. transmits Dengue viruses that cause DHF. Bangsring, Banyuwangi is one of the endemic areas of Malaria, while Jember is one of endemic areas of DHF. Two species of Malaria vectors i.e Anopheles vagus and Anopheles sundaicus have been found from several samplings which were conducted in Bangsring region. This mosquitoes have been previously identified as a major vector for malaria. Aedes aegypti and Aedes albopictus, which were major vectors for DHF, have been easily found in Jember during our sampling for any season of the year. This research wanted to differentiate the morphological characteristic of those vectors for malaria and Dengue. Adult mosquitoes can be distinguished from one another by characterizing their morphological features. Palpi, wings, and legs characteristics are commonly used as determination keys in Anopheles sp., while thoraxes and legs characteristics are used as identification keys in Aedes sp. Morphological identification is considered as the basic necessity in understanding and determining bionomic of mosquito vector. This is very important in developing effective and efficient mosquito vector control strategies, which is also an important step to prevent the death risks associated with Malaria and DHF cases.
THE IMMUNOGLOBULIN (IgG) ANALYSIS OF IMPLANTATION MICE (Mus musculus L.) POST OVARIECTOMY AFTER TREATMENT OF BLACK SOYBEAN FLOUR EXTRACT (Glycine soja) Yenny Febriana Ramadhan Abdi; Mahriani Mahriani; Kartika Senjarini; Rike Oktarianti
BIOEDUKASI Vol 19 No 1 (2021)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v19i1.21099

Abstract

Black soybean is one type of plant that has estrogenic activity, it can act as a source of natural exogenous estrogen. Decreased estrogen hormone in the body due to ovariectomy and menopause, it affect on immune response (IgG). The purpose of this study was to determine the effect of black soybean extract on immune response (IgG) of implantation mice post ovariectomy. Twenty eight mice were grouped into 4 groups, group 1 (negative control), group 2 (positive control with the administration estradiol concentration 50 ppm), group 3 and 4 were treatment group with the administration of black soybean flour extract doses of 0.31 g/ml and 0.63 g/ml. The data were analyzed using One Way Anova and Duncan Multiple Range Test (DMRT) (p<0,05). The treatment with administration of black soybean flour extract for 10 days in implantation mice post ovariectomy showed that a dose of 0.31 g/ml and dose of 0.63 g/ml had an effect on increasing significantly the immune response (IgG), i.e 0,12987and 0,12996 respectively.
PURIFICATION OF 31 AND 67 kDa PROTEIN FRACTION FROM SALIVARY GLAND OF Aedes Albopictus (SKUSE) (DIPTERA: CULLICIDAE) Syubbanul Wathon; Rike Oktarianti; Nuril Azizah; Yasir Mubarok; Riana Agatha Listiani; Kartika Senjarini
BIOEDUKASI Vol 19 No 1 (2021)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v19i1.18892

Abstract

Aedes albopictus mosquito is a potential vector for Dengue Haemorrhagic Fever (DHF) which transmits Dengue virus during blood feeding. The success of the blood feeding process is aided by the biological activity of proteins in the salivary glands of Aedes albopictus. There are 30 types of proteins from the salivary glands of Aedes albopictus which are carried along blood feeding process. Proteins in the salivary glands act as vasodilator and immunomodulator. Previous studies have identified two immunogenic proteins from the salivary glands of Aedes albopictus with molecular weight of 31 and 67 kDa. Further research on the biological function of these proteins requires its purified protein to better specify the target to developing a dengue vaccine. The objective of this study was to obtain 31 and 67 kDa purified proteins by implementation of electroelution and dialysis purification. The 31 and 67 kDa protein was successfully purified by this method. This has been confirmed by a single band visualization after SDS-PAGE analysis
PLATELET AGGREGATION IN VITRO ANALYSIS OF 67 kDa IMMUNOGENIC PROTEIN FRACTION FROM Aedes albopictus SALIVARY GLAND (SKUSE) (DIPTERA: CULICIDAE) Rike Oktarianti; Syubbanul - Wathon; Intan Fitri Indrasari; Nadya Rismana Fitriani; Kartika Senjarini
BIOEDUKASI Vol 18 No 2 (2020)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v18i2.18891

Abstract

Cardiovascular disease is caused by impaired heart and blood vessel function. Coronary heart disease includes acute coronary syndrome due to narrowing of the coronary arteries. Aspirin is an anti-platelet drug which is commonly used for primary and secondary prevention of coronary heart disease. The effectiveness of aspirin has limitations because 10-20% of patients who use aspirin continue to experience vascular blockage. The haematophagus arthropods salivary glands contain apyrase which can inhibit platelet aggregation and thus a potential candidate for anti-platelet drug discovery. Our studies from salivary gland protein extract of Aedes albopictus found a 67 kDa protein fraction which has a similar molecular weight range from the previously identified apyrase of other mosquitoes vectors. The purpose of this study was to determine the apyrase potential capacity of 67 kDa protein fraction from salivary gland of Ae. albopictus. The present study employed inhibition percentage of platelet aggregation method in determining its apyrase activity. Aspirin was used as a positive control with 2 different concentrations of 0.1 mg/mL and 2 mg/mL. PBS 1mM pH 7.4 was used as a negative control treatment, while negative control without treatment only involved the addition of PRP and ADP. The inhibition percentage activity from the 67 kDa sample ranged from 3-37% whereas the total protein extract comprised of only 1%. The positive control of aspirin was 0.1 mg/mL and 2 mg/mL, resulting in inhibition percentage of 5% and 17%, respectively. The data showed that the inhibition percentage of platelet aggregation from protein 67 kDa is generally higher than those of total salivary gland protein extract as well as positive control. This indicated that the 67 kDa protein has a potential apyrase activity.
USING sma-its2 PRIMER FOR ITS2 (Internal Transcribed Spacer-2)-BASED MOLECULAR CHARACTERIZATION OF Anopheles minimus FROM KULONPROGO, YOGYAKARTA - INDONESIA Syubbanul Wathon; Berlian Permata Dewi Erlambang; Naura Paramitha Cindy Ardyah; Devi Astikaningrum; Rike Oktarianti; Kartika Senjarini
BIOEDUKASI Vol 20 No 1 (2022)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v20i1.31911

Abstract

Malaria is a public health threat caused by the Plasmodium infection transmitted by the Anopheles mosquito. Malaria vector control is highly dependent on the ability to determine mosquito species' vectorial and bionomic capacity. Species identification based on morphological characteristics as well as DNA-barcoding approaches is a very important step in determining vectorial capacity. Our research group has redesigned a new ITS2 primer, namely, sma-its2, which is specifically able to identify the Anopheles (An.) mosquito vector but cannot identify other mosquito vectors. This study wanted to test this primer's specificity further for identification of other Anopheles mosquitoes. We used An. minimus collected from Kulonprogo, Yogyakarta – Indonesia. The methods used in this research are as follows: landing collection, morphological identification, isolation of genomic DNA, PCR (Polymerase Chain Reaction), PCR product purification, sequencing, and data analysis. An. vagus from Bangsring, Banyuwangi – Indonesia, which had previously been identified using the same primary, was used as a positive control. The results of the morphological analysis showed that both species were in accordance with the vector identification key used in this study. The molecular analysis showed that the sma-its2 primer could amplify the ITS2 sequence of An. vagus and An. minimus, producing 650 – 700 bp. However, further analysis of the ITS2 sequences of both species, resulted in the same species, namely An. vagus, with a different accession number in GenBank. This showed that the sma-its2 primar can be used to identify An. vagus but cannot be used to identify An. minimus. Analysis of the primer position in the ITS2 sequences showed the presence of 3 nucleotides in the forward sma-its2 primer that was not recognized by the An. minimus sequences and thus, hinder the successful identification of these species.
ANOPHELES MICROBIOTA IN MALARIA VECTOR AND THE EFFECT ON PARASITE TRANSMISSION Dita Paramytha Agustin; Berlian Permata Dewi Erlambang; Cizein Tri Cahyanti; Syubbanul Waton; Rike Oktarianti; Kartika Senjarini
BIOEDUKASI Vol 19 No 2 (2021)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v19i2.25165

Abstract

Malaria is a vector-transmitted disease with a high number of cases. Plasmodium parasites are transmitted from the body of the Anopheles mosquito to the host through several mosquito organs, including the salivary glands and the midgut. The salivary glands and the midgut, apart from being a breeding ground for parasites, are known from several studies that various types of microbiota inhabit these two organs. The group of bacteria is the most widely known to be associated with Anopheles mosquitoes. The bacteria found were dominated by Gram-negative bacteria, with Pseudomonas being the most common, followed by Aeromonas, Asaia, Comamonas, Elizabethkingia, Enterobacter, Klebsiella, Pantoea, and Serratia. The group of Gram-positive bacteria was represented by the genera Bacillus, Streptococcus, Lactobacillus, and Staphylococcus. Each species of Anopheles has a different composition of associated bacteria. Anopheles-associated bacteria currently receive much attention because of their role in fighting Plasmodium infection. The ability of malaria vector-associated bacteria to inhibit and fight Plasmodium infection is divided into three mechanisms. Bacteria can triger an immune response through the Immune-deficiency (IMD) pathway, which causes active anti-Plasmodium and the production of antimicrobial peptides, increasing ROS synthesis and microbiota, which trigger hemocyte differentiation to protect against Plasmodium. This function related to inhibiting Plasmodium development needs further research so that later it can become an option for vector-based malaria control without damaging the sustainability of the environment.
Co-Authors Adrial Adrial Ahmad Tosin Ajeng Maharani Sri Pananjung Ajeng Maharani Sri Pananjung, Ajeng Maharani Ali Machrus Anak Agung Gede Sugianthara Anam, Khairul Ari Satia Nugraha Arif Setiawan Bagus Setiawan Berlian Permata Dewi Erlambang Budayatin Budayatin Cizein Tri Cahyanti Devi Astikaningrum Devi Astikaningrum Dewi Masruroh Didik Sulistyanto DINA FITRIYAH Dita Paramytha Agustin Dwi Esti Febriyantiningsih Esti Utarti Evi Umayah Ulfa Fitria Mutiah Hidayat Teguh Wiyono Husnul Hotimah Ihsanul Azmi Intan Fitri Indrasari Izzay Afkarina Kahar Muzakhar Khasanah, Rochmatul Nuryu Lailly Nur Uswatul Hasanah Loeki Enggar Fitri Mahful Septiawan Mahriani Mahriani Maulana Jauharil Habib Miatin Alvin Septianasari Mochtar Gunawan Wibisono Moh Mirza Nuryady Moh Mirza Nuryady, Moh Mirza Muhammad Khalid Abdullah Mutiah, Fitria Nadya Rismana Fitriani Naura Paramitha Cindy Ardyah Naura Paramitha Cindy Ardyah Nuril Azizah Nuryatmaja Gora Pawana Purwatiningsih Purwatiningsih Ratis Nour Sholichah Renam Putra Arifianto Rhena Schumann Riana Agatha Listiani Rike Oktarianti Sattya Arimurti Silvya Fitri Nur Azizah Siti Fat'hiyatul Azkiyah Sri Mumpuni Wahyu Widajati Sri Mumpuni Wahyu Widajati, Sri Mumpuni Wahyu Sugeng Setyo Utomo Sugeng Setyo Utomo, Sugeng Setyo Sutoyo Sutoyo Syubanul Wathon Syubbanul Wathon, Syubbanul Syubbanul Waton Syubhanul Wathon Teguh Wahju Sardjono Teguh Wahju Sardjono Ulf Karsten Widya Cahyadi Yasir Mubarok Yenny Febriana Ramadhan Abdi Yunita Armiyanti