The purpose of this study was to determine the activity of spray preparations of marigold flower essential oil (Tagetes erecta L.) on the Aedes aegypti mosquito. Marigold flower essential oil using water distillation method using aquadest and rabbits as test animals. The study was conducted by taking five treatments, namely one negative control group K(-), three treatment groups with variations in concentration (P1) 2.5%, (P2) 5%, P(3) 10% and one positive control group K( +). The results showed that repellent spray had repulsion, control (-) was 50.67%, 2.5% concentration was 68.33%, 5% concentration was 78.67%, 10% concentration was 96% and control (+ ) by 100%. The results of the protection test showed that the repellent spray containing marigold flower essential oil had the highest activity at a concentration of 10%. Based on the results of statistical analysis showed that the calculated f is greater than f table 5% and 1% and the results of the follow-up test of SNK (Newman Keuls) showed that the concentration of 10% was not significantly different from the control (+).