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Comparison of The Number of Macrophage in Full Thickness Wound Incision between Merremia mammosa Extract Treatment and NaCl in Male Wistar Rats Hidayat, Fajar Kurniawan; Elfiah, Ulfa; Sofiana, Kristianningrum Dian
Journal of Agromedicine and Medical Sciences Vol 1, No 1 (2015)
Publisher : Medical Faculty of Jember University (Fakultas Kedokteran Universitas Jember)

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Abstract

The incidence of diabetes mellitus in Indonesia continues to increase. There are some complications in the diabetic condition, one of which is diabetic ulcer. Diabetic ulcers in diabetes patient can increase the risk of amputation and expensive treatment costs, so the alternative treatmeant such as Merremia mammosa wich has antiinflamatory and antidiabetic is needed. This study aimed to determine the comparison of the number of macrophage in the incisional wound in hyperglycemic male wistar rats between treatment with Merremia mammosa extract and NaCl. The in vivo test was done by creating wound incision on the mice backs and treated with gentamycin ointment 5% in a positive control group, NaCl in a negative control group and Merremia mammosa extract in a dose of 100m), 200mg, 400mg in treatment group. The result obtained by counting the number of macrophage in histopatholgy examination. The result showed the number of macrophage were 0,36 cells/field of view in a negative control group, 0,52 cells/field of view in a positive control group, 0,48 cells/field of view in all Merremia mammosa treatment groups. The data analysis showed no significant difference with p-value of 0,729. In conclusion, there was no significant difference between the used of Merremia mammosa extract and NaCl on full thickness incisional wounds of hyperglycemic male wistar rats. Keywords: Diabetes Mellitus, Merremia mammosa extract, incisional wound
Hubungan Paparan Pestisida dengan Kadar SGOT dan SGPT Petani di Desa Pakis Kabupaten Jember Zahrox, Iin Fatimatus; Hairrudin, Hairrudin; Sofiana, Kristianningrum Dian
Jurnal Kesehatan Lingkungan Indonesia Vol 20, No 1 (2021): April 2021
Publisher : Master Program of Environmental Health, Faculty of Public Health, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkli.20.1.47-52

Abstract

Latar belakang: Hepar merupakan salah satu organ yang rentan terhadap paparan pestisida. Akumulasi pestisida di dalam hepar tidak dapat diuraikan maupun diekskresikan. Penumpukan pestisida pada hepar memicu peningkatan jumlah radikal bebas dan menyebabkan gangguan permeabilitas membran sel hepatosit. Kondisi tersebut mengakibatkan kerusakan sel hepatosit dan meningkatkan kadar SGOT maupun SGPT. Desa Pakis merupakan salah satu desa penghasil padi terbesar di Kabupaten jember dengan mayoritas penduduk bekerja sebagai petani. Penelitian ini bertujuan untuk menganalisis hubungan paparan pestisida terhadap kadar SGOT dan SGPT pada petani di Desa Pakis Kabupaten Jember.Metode: Penelitian ini merupakan penelitian observasional analitik dengan desain penelitian cross sectional. Sampel pada penelitian ini adalah petani padi Desa Pakis yang menggunakan pestisida organofosfat atau karbamat Pengambilan sampel dilakukan menggunakan teknik non probability sampling dengan metode accidental sampling. Data diperoleh melalui wawancara dan pengukuran kadar asetilkolinesterase, SGOT dan SGPT menggunakan fotometer.Hasil: Sampel berjumlah 30 orang. Tingkat paparan pestisida yang tinggi ditunjukkan oleh kadar asetilkolinesterase <75% ditemukan pada 23,33% dari sampel. Sebagian besar berusia > 50 tahun dan telah bekerja lebih dari 10 tahun. Peningkatan kadar SGOT dan SGPT didapatkan masing-masing sebesar 33,33% dan 23,33%. Hasil penelitian menunjukkan bahwa tidak ada hubungan yang signifikan antara paparan pestisida dengan kadar SGOT (p = 0,320) dan SGPT (p = 604).Simpulan: Tidak ada hubungan antara paparan pestisida dengan kadar SGOT dan SGPT pada petani di Desa Pakis Kabupaten Jember. ABSTRACT Title: The Correlation of Pesticides Exposure with Farmer’s SGOT and SGPT level in Pakis Village, Jember RegencyBackground: The Liver is one of the organs that are vulnerable to pesticide exposure. Accumulated pesticides in the liver cannot be broken down or excreted. Pesticides that build up in the liver triggers an increase in the number of free radicals and cause an impaired hepatocyte membrane permeability. These conditions result in damage to hepatocyte cells and an increase in SGOT/SGPT levels. Pakis village is one of the biggest paddy producers in Kabupaten  Jember with the majority of its people works as farmers. This research aims to analyze the correlation of pesticide exposure and SGOT/SGPT levels among farmers in Pakis village, Jember regency. Method: This research is an analytic observational study using a cross-sectional research design. Samples were farmers who had been using organophosphate or carbamate pesticides. Sample collected using a nonprobability sampling technique with the accidental sampling method. Data obtained through interviews and measurements of acetylcholinesterase, SGOT, and SGPT levels using a photometer.Result: A number of 30 subjects participated. A high degree of pesticide exposure as indicated by acetylcholinesterase level of <75% was encountered in 23.33% of samples. Most samples were > 50 years old and had worked for more than 10 years.. Increased SGOT and SGPT levels were encountered in 33.33% and 23.33%, respectively. This research has shown that there was no significant correlation between pesticide exposure with SGOT (p=0,320) and SGPT levels (p=604).Conclusion: No significant correlations were found between pesticides exposure with SGOT and SGPT levels in farmers in Pakis village, Jember regency.
Hubungan Jumlah Rakaat Salat Dhuha terhadap Peningkatan Detak Jantung Prasetya, Moch.Arief; Hasan, Muhammad; Sofiana, Kristianningrum Dian
Journal of Islamic Medicine Vol 5, No 2 (2021): JOURNAL OF ISLAMIC MEDICINE EDISI SEPTEMBER 2021
Publisher : Faculty of Medicine and Health Science, Universitas Islam Negeri Maulana Malik Ibrahim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jim.v5i2.12326

Abstract

Salat is a muslim worship performed with the movement and reading. Salat types of physical activity with light intensity that can affect heart rate. Heart rate increases in physical activity according to the increase in intensity. This study aims to determine correlation of the number rakaat salat dhuha for increased heart rate. This type of research is analytic observational with a quasi experimental design. The total sample of 42 is divided into 6 treatments, each treatment contains 7 respondents. The sampling technique used a simple random sampling method. The research was conducted from November 2020 to April 2021 using respondents' observations. Data analysis used One Way Anova test and Pearson test. The results of the One Way Anova test, which is a significance value of 0.001 indicating that there is a difference in the increase in heart rate prayer intensity and the results of the Pearson test analysis, namely the value of r or the correlation coefficient of 0.637 and a significant value of=0.00 shows a strong relationship between prayer intensity and an increase in heart rate. The conclusion of this study is that the addition of rakaat salat dhuha increases the heart rateKeyword: Heart Rate, Salat Dhuha
Efek Pemberian Vitamin E (d-α-tocopherol) terhadap Memori Kerja Spasial Tikus (Rattus novergicus) Remaja yang Diinduksi Etanol Shera Nadhila Setyo Bisono; M. Ihwan Narwanto; Kristianningrum Dian Sofiana
Pustaka Kesehatan Vol 2 No 2 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

During neurogenesis process, nerve cells especially hippocampus susceptible to damage when exposed to ethanol. Vitamin E is an antioxidant and capable to protect nerve cells damage. This study aimed to determine the effect of vitamin E (d-α-tocopherol) on spatial working memory of adolescent rats induced with ethanol. The study used 30 days-old rats (Rattus novergicus), weight between 50-100 grams. Total of 25 rats were divided into 5 groups: 2 control groups and 3 treatment groups. K1/K(-) was normal group without ethanol and vitamin E, and K2/K(+) were induced with ethanol. The treatment groups were given ethanol and vitamin E at a dose of 100mg/kgBW; 200 mg/kgBW; 400 mg/kgBBW respectively for P1, P2 and P3. After treatment for 14 days, the rats were tested for spatial working memory using the Radial Arm Maze (RAM) for 10 consecutive days. Spatial working memory was observed by the total number of right arm option which were entered by rats. The average number of right arm maze option for 10 days, were; K (-): (7 ± 0.52), K (+): (3.98 ± 0.66), P1: (4 , 56 ± 0.41), P2: (6.76 ± 1.01) and P3 (6.38 ± 0.63). In conclusion, there was an effect of vitamin E at a dose of 200 mg/kgBW and 400 mg KgBW on spatial working memory of ethanol-induced adolescent rats.   Keywords: ethanol, vitamin E, spatial memory, Radial Arm Maze
Efek Preventif Ekstrak Etanol Kopi Robusta (Coffea canephora) terhadap Peningkatan Kadar Kolesterol Total Tikus Jantan Galur Wistar yang Diinduksi Kuning Telur Yan Agus Achtiar; Hairrudin Hairrudin; Kristianningrum Dian Sofiana
Pustaka Kesehatan Vol 2 No 3 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Kopi robusta mengandung asam klorogenat (CGA) sekitar 7-14%. Asam klorogenat diduga dapat mencegah penyerapan kolesterol diusus. Penelitian ini bertujuan untuk membuktikan efek preventif ekstrak etanol kopi robusta (Coffea canephora) terhadap peningkatan kadar kolesterol total tikus. Penelitian ini adalah penelitian eksperimental laboratorium pre-post-test dengan kelompok kontrol. Dua puluh emapt ekor tikus wistar jantan usia 3-4 bulan, dibagi menjadi kelompok perlakuan K1 diinduksi kuning telur, kelompok K2 diinduksi kuning telur dan ekstrak kopi dosis terapi (0,5 mL / ekor), kelompok K3 diinduksi kuning telur dan simvastatin, kelompok K4 diinduksi kuning telur dan ekstrak kopi dosis tinggi (1 mL / ekor) selama 35 hari. Pada awal dan akhir perlakuan diambil serum darah untuk mengetahui kadar kolesterol total darah menggunakan menggunakan metode CHOD-PAP. Dalam waktu 35 hari, pemberian ekstrak kopi robusta tidak mencegah kenaikan kadar kolesterol total. Sehingga dapat disimpulkan bahwa tidak ada efek preventif ekstrak etanol kopi robusta (Coffea canephora) terhadap peningkatan kadar kolesterol total tikus wistar jantan yang diinduksi kuning telur.   Kata kunci: Hiperkolesterolemia, asam klorogenat, kolesterol total serum
Jumlah Makrofag pada Luka Insisi Full Thickness yang diberi Ekstrak Umbi Bidara Upas (Merremia mammosa (Lour)) pada Tikus Wistar Jantan (The Number of Macrophage in Full Thickness Wound Incision after Treatment using Merremia mammosa Extract in Male Wist Fajar Kurniawan Hidayat; Ulfa Elfiah; Kristianningrum Dian Sofiana
Pustaka Kesehatan Vol 3 No 3 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

One important complication in the diabetic patient is diabetic ulcer. Diabetic ulcers in diabetes patient can increase the risk of amputation and expensive treatment costs, so the alternative treatmeant such as Merremia mammosa which has antiinflamatory and antidiabetic is needed. This study aimed to determine the comparison of the number of macrophage in the incisional wound in hyperglycemic male wistar rats between treatment with Merremia mammosa extract and NaCl. The in vivo test was done by creating wound incision on the mice backs and treated with gentamycin ointment 5% in a positive control group, NaCl in a negative control group and Merremia mammosa extract in a dose of 100m), 200mg, 400mg in treatment group. The result obtained by counting the number of macrophage in histopatholgy examination. The result showed the number of macrophage were 0,36 cells/field of view in a negative control group, 0,52 cells/field of view in a positive control group, 0,48 cells/field of view in all Merremia mammosa treatment groups. The data analysis showed no significant difference with p-value of 0,729. In conclusion, there was no significant difference between the used of Merremia mammosa extract and NaCl on full thickness incisional wounds of hyperglycemic male wistar rats.   Keywords: Diabetes Mellitus, Merremia mammosa extract, incisional wound
Respon Imunogenitas Antibodi Poliklonal IgY terhadap Protein Adhesi Pili 95 kDa Shigella dysenteriae asihanti rosita ferdiana; Enny Suswati; Kristianningrum Dian Sofiana
Pustaka Kesehatan Vol 2 No 2 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Shigella dysenteriae is the most frequently cause of deaths of dysentery cases in infants and toddlers. Shigella dysenteriae has a pili that act as an adhesin molecule and will detect in human body as antigens which is involved in producing antibodies. This study was conducted to prove that Shigella dysenteriae pili adhesion protein 95 kDa is immunogenic. This was an experimental laboratory study with quasy experimental design in vitro. The study began with mice erythrocyte cell isolation, IgY polyclonal antibodies isolation, mice intestinal enterocytes cell isolation, hemagglutination inhibition test, and adhesion inhibition test. The haemagglutination inhibition test showed that the pili adhesion protein 95 kDa of S. dysenteriae able to inhibit the haemagglutination of erythrocytes mice Balb/C up to ½ dilution. While, the adhesion inhibition test revealed that the fewer of antibody concentration on enterocytes, the greater bacteria attached. According to the linear regression test results, the index adhesion value increased along with the decreased of antibody concentration. In conclusion, pili adhesion protein 95 kDa from S. dysenteriae was immunogenic and could inhibit the hemagglutination and adhesion between S. dysenteriae and mice enterocytes cells.   Keywords: immunogenicity, IgY polyclonal antibody, Shigella dysenteriae, pili adhesion protein.
Pengaruh Terapi Kombinasi Ekstrak Etanol Mentimun (Cucumis sativus) dan Vildagliptin terhadap Penurunan Kadar Glukosa Darah Tikus Wistar yang Diinduksi Aloksan (The Effect of Combination Treatment of Cucumber (Cucumis sativus) Ethanolic Extract and Vilda Chita Setya Widyani; Sugiyanta Sugiyanta; Kristianningrum Dian Sofiana
Pustaka Kesehatan Vol 3 No 1 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Diabetes mellitus is a metabolic disease characterized by hyperglycemia. Effective management is needed. Cucumber is one of traditional medicine contains saponins with antihyperglycemic effects. This study aimed to determine the differences between combination therapy of cucumber ethanolic extract and vildagliptin and monotherapy of cucumber ethanolic extract or vildagliptin to decrease the blood glucose. The rats were injected with alloxan 125 mg/kgBW. There were 5 groups: K1 was normal group, K2 was a group of diabetic rats without treatment, K3 was a group of diabetic rats with cucumber ethanolic extract (200 mg/kgBW), K4 was a group of diabetic rats with vildagliptin treatment (1,8 mg/200 gBW), K5 was a group of diabetic rats with combination treatment of cucumber ethanolic extract (200 mg/kgBW) and vildagliptin (1,8mg/200 gBW). After 14 days, fasting blood glucose (FBG) was measured. The result showed that the mean FBG after treatment (FBG 2) of K1, K2, K3, K4, and K5 were (82,4; 277,2; 188,2; 199,2; 180,6) mg/dL, respectively. In conclusion, the potential of cucumber ethanolic extract and vildagliptin in combination treatment was more effective to decrease the blood glucose levels than both monotherapy of cucumber ethanolic extract or vildagliptin in Wistar rats.   Keywords: Diabetes Mellitus, Cucumber, Vildagliptin, Blood Glucose Levels, Alloxan
Correlation between Cholinesterase Levels and Blood Glucose Levels in Farmers Exposed to Organophosphate Pesticide in Desa Sukorambi Kabupaten Jember Sofiannisa Achmadila; Kristianningrum Dian Sofiana; Yudha Nurdian
Journal of Agromedicine and Medical Sciences Vol 5 No 3 (2019)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v5i3.9652

Abstract

The use of organophosphate pesticides by farmers can cause long-term health risks, one of which is an increase in blood glucose levels. This study aims to determine whether there is a relationship between the levels of cholinesterase as an indicator of exposure to organophosphate pesticides and blood glucose of farmers in Sukorambi Village, Jember Regency. Secondary data in the form of general characteristics of the sample were obtained through interviews, while the primary data was obtained from examining the levels of cholinesterase and blood glucose levels of farmers at the Biochemical Laboratory of the Faculty of Medicine, Jember University. The research method is observational-analytic with cross-sectional study design. Based on the results of statistical analysis using the Spearman correlation test (p = 0.05), it was concluded that there was no significant relationship between the two variables (p = 0.0802).
Relationship of Cholinesterase Activity with Blood Glucose Levels Due to Pesticide Exposure to Farmers in Mlokorejo Village Muhammad Ryznar Faisal Nur Luqmani; Kristianningrum Dian Sofiana; Bagus Hermansyah
Journal of Agromedicine and Medical Sciences Vol 7 No 1 (2021)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v7i1.20576

Abstract

Increase in blood glucose levels can be caused by the long-term use of pesticides by farmers. This study aims to determine whether there is a correlation between the activity of cholinesterase as an indicator of exposure to pesticides and blood glucose of farmers in Mlokorejo Village, Jember Regency. The research method is observational-analytic with a cross-sectional study design. Data in the form of general characteristics of the sample were obtained through interviews, while the data of the activity of cholinesterase and blood glucose levels of farmers were obtained at the Biochemical Laboratory of the Faculty of Medicine, Jember University. The sample size of this study is 30 samples. All of the samples are having an increased activity of cholinesterase. Most of the samples are having normal blood glucose levels. Based on the results of statistical analysis using the Spearman correlation test (a= 0.05), it was concluded that there was no significant correlation between the two variables (p = 0,191). Keywords: blood glucose, cholinesterase, pesticides