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All Journal JURNAL PHOTON Jurnal Teknik Kimia USU
Riryn Novianty
Universitas Riau
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Nursing Physics

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MEMPELAJARI DAYA INHIBISI ION CU2+ DAN CA2+ SERTA POLA INHIBISINYA TERHADAP ENZIM AKONITASE YANG DIISOLASI DARI BUAH KALIKIH ALANG (RICINUS COMMUNIS) Riryn Novianty; Zulkarnain Chaidir; Elida Mardiah
Sistem Informasi Vol 5 No 1 (2014): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v5i1.411

Abstract

Penelitian tentang inhibisi aktivitas enzim akonitase dengan menggunakan inhibitor ion Cu2+ dan Ca2+ telah dilakukan. Enzim akonitase diisolasi dari buah Ricinus communis dengan menggunakan aseton dingin. Untuk menentukan aktivitas dari akonitase digunakan metoda pengukuran substrat asam sitrat sisa berdasarkan metoda LStahre’s test. Kadar protein ditentukan dengan menggunakan metoda Lowry. Optimasi dari enzim akonitase didapatkan dari hasil pH optimum 7,3, temperatur optimum 40 oC, dan konsentrasi substrat 1 %. Dari kurva Lineaweaver Burk diperoleh harga Km = 0,104 M. Sedangkan untuk mengetahui daya pola inhibisi ion Cu2+ dan Ca2+ dilakukan dengan menvariasikan konsentrasi dari masing-masing ion tersebut. Daya inhibisi ion Cu2+ dengan konsentrasi 0,1 M adalah 93,347 % dan daya ion Ca2+ pada konsentrasi 0,1 M adalah 60,124 %. Hasil tersebut menunjukkan pola inhibisi yang sama yakni inhibisi non kompetitif dan masing-masing ion memiliki daya inhibisi yang berbeda.
Bakteri Indigen Pendegradasi Hidrokarbon Minyak Bumi di Kabupaten Siak Provinsi Riau Riryn Novianty; Saryono; Amir Awaluddin; Nova Wahyu Pratiwi
Jurnal Teknik Kimia USU Vol. 9 No. 1 (2020): Jurnal Teknik Kimia USU
Publisher : Talenta Publisher (Universitas Sumatera Utara)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (475.741 KB) | DOI: 10.32734/jtk.v9i1.3834

Abstract

The process of producing, refining and transporting petroleum can cause pollutants that are harmful to the environment and the living things that surround them. Indigenous microorganisms can be a solution to degrade hydrocarbons that are difficult to degrade. This study aims to isolate the indigenous bacteria from pollutant-contaminated soil and test its effectiveness in degrading hydrocarbons. The research began with soil sampling at the Joint Operating Agency of PT Siak Bumi Pusako-Pertamina Hulu Siak Regency, Riau Province with a purposive sampling method. The next step is the isolation of indigenous bacteria, testing the parameters of pH, Optical density (OD) and CO2 levels during the 16 incubation periods and determining the highest percentage of biodegradation. The results showed that Pseudomonas sp. with a code BTM2 (Bakteri Tanah Minyak 2) had the largest percentage (52.20%) after 16 days of incubation under optimum conditions to degrade the total hydrocarbon of petroleum. Pseudomonas sp. BTM2 is the most effective isolate to degrade hydrocarbons.
Co-Authors Amir Awaluddin Elida Mardiah Nova Wahyu Pratiwi Saryono Zulkarnain Chaidir