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All Journal HAYATI Journal of Biosciences Jurnal Teknologi Dan Industri Pangan VALENSI Jurnal Kimia (Journal of Chemistry) Jurnal Veteriner Biotika: Jurnal Ilmiah Biologi Bionatura Indonesian Journal of Applied Sciences Jurnal Ilmu Dasar Jurnal Natur Indonesia Majalah Kedokteran Bandung Cropsaver : Journal of Plant Protection Jurnal Penelitian Hasil Hutan Indonesian Journal of Cancer Chemoprevention Journal of Mathematical and Fundamental Sciences Indonesian Journal of Pharmaceutical Science and Technology STOMATOGNATIC- Jurnal Kedokteran Gigi Indonesian Journal of Science and Technology Dentino: Jurnal Kedokteran Gigi The Indonesian Biomedical Journal Padjadjaran Journal of Dentistry Jurnal Bioteknologi & Biosains Indonesia (JBBI) JURNAL PENDIDIKAN JASMANI DAN OLAHRAGA Al-Kimia Chimica et Natura Acta ALCHEMY Jurnal Penelitian Kimia Indonesian Journal of Chemistry Akta Kimia Indonesia Jurnal Kartika Kimia B-Dent, Jurnal Kedokteran Gigi Universitas Baiturrahmah Molekul: Jurnal Ilmiah Kimia Jurnal Ilmu dan Teknologi Kayu Tropis Jurnal Permukiman Jurnal Penelitian Hasil Hutan Jurnal Ilmiah Berkala: Sains dan Terapan Kimia Denta: Jurnal Kedokteran Gigi Makara Journal of Science Jurnal Ilmiah Sains Molekul: Jurnal Ilmiah Kimia
Unang Supratman
Departemen Kimia, Fakultas MIPA, Universitas Padjadjaran, Bandung.
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Veterinary

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In Vitro Anti-Cancer Alkaloid and Flavonoid Extracted from the Erythrina variegata (Leguminoseae) Plant Herlina, Tati; Supratman, Unang; Subarnas, Anas; Sutardjo, Supriyatna; Amien, Suseno; Hayashi, Hideo
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Erythrina plants, locally known as “dadap ayam”, are higher plant species and have been used as a folk medicine for treatment of cancer. To prove the effectiveness of the leaves and stem bark of E. variegata as an anti-cancer agent, the assay in this research was focused on in vitro  test  towards  breast  cancer  cell  T47D.  In  the course  of  our  continuing  search  for novel anti-cancer agent from Erythrina plants, the methanol extract of the leaves and stem bark of  E. variegata  showed  significant  anti-cancer  activity  against  breast  cancer  cell  T47D  in  vitro  using the Sulphorhodamine B (SRB) assay. By using the anti-cancer activity to follow the separations, the methanol extract was separated by combination of column chromatography. The chemical structure  of  an  anti-cancer  compounds  were  determined  on  the  basis  of  spectroscopic evidence  and  comparison  with  the  previously  reported  and  identified  as  an  erythrina  alkaloid (1)  and  isoflavonoid  (2).  Compounds  (1-2)  showed  anti-cancer  activity  against  breast  cancer cell  T47D  used  with  IC50  of    1.0  and  3.3  µg/mL,  respectively.  This  results  strongly  suggested that E. variegata is promising sources for anti-cancer agents.Keywords: Anti-cancer, Erythrina variegata, Leguminoseae
Ethanol extract of mangosteen (Garcinia Mangostana Linn) peel effect in inhibiting the growth of human tongue cancer cells Supri’s Clone 1, invitro Suanto, Edi; Oewen, Roosje Rosita; Sasmita, Inne Suherna; Supriatno, S.; Supratman, Unang
Padjadjaran Journal of Dentistry Vol 23, No 2 (2011): July
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.616 KB) | DOI: 10.24198/pjd.vol23no2.14022

Abstract

The incidence of tongue cancer in Indonesia reached 1.01% of all cancers and 42% of oral cavity cancer. Tongue cancer therapies including chemotherapy, radiotherapy, surgery, and all three combined therapy. Search for anti-cancer drugs currently switched on herbal plants, one of which is the mangosteen. Has the properties of mangosteen peel extract inhibited the growth of cancer cells. The purpose of the study, obtain IC50 of ethanol extract of mangosteen peel in inhibiting the growth of human tongue cancer cells SP-C1. Research carried out on 96 preparations of human tongue cancer SP-C1 were incubated with ethanol extract of mangosteen peel, preparations were classified in two groups of incubation time (24 hours and 48 hours) and each group will be given preferential treatment over 6 randomly different concentrations: 0 (control), 62.5 μg/mL, 125 μg/mL, 250 μg/mL, 500 μg/mL and 1000 μg/mL. Model experiments were 2 x 6 factorial experiment with eight replication for each cell. Test results with ANAVA, incubation (24 and 48 hour) SP-tongue cancer cells with various concentrations of C1 ethanol extract of mangosteen peel gives a highly significant, indicating differences cancer cell growth inhibition. Incubation time factor showed the long incubation effect on cancer cell growth inhibition. Furthermore, by Newman Keuls test, showed 500μg/mL concentrations of 24-hour incubation had the best effect. Conclusion of the study of ethanol extract of mangosteen peel could achieve with IC50 values of cell growth resistance 50.3% at a concentration of 500 μg/mL and an incubation time of 24 hours.
ISOLASI DAN MOLECULAR DOCKING SENYAWA 6,7-DIHIDRO-17-HIDROKSIERISOTRIN DARI DAUN DADAP BELENDUNG (Erythrina poeppigiana) TERHADAP AKTIVITAS SITOTOKSIK ANTIKANKER PAYUDARA MCF-7 Mardianingrum, Richa; Herlina, Tati; Supratman, Unang
Chimica et Natura Acta Vol 3, No 3 (2015)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.399 KB) | DOI: 10.24198/cna.v3.n3.9213

Abstract

Erythrina (Leguminose) memiliki jumlah alkaloid paling banyak jika dibandingkan dengan senyawa metabolit sekunder lainnya, sehingga tumbuhan Erythrina dikenal dengan sebutan alkaloid eritrina. Erythrina yang paling banyak tumbuh di Indonesia adalah E. poeppigiana. Tujuan penelitian ini adalah untuk mengungkapkan senyawa aktif antikanker payudara MCF-7 secara in silico yang terdapat di dalam daun E. poeppigiana. Alkaloid dipisahkan dan dimurnikan melalui tahapan ekstraksi, fraksinasi, pemisahanan dan pemurnian yang dipandu dengan uji Dragendorff, senyawa alkaloid yang diperoleh diuji antikanker secara in silico terhadap reseptor EGFR2 yang bekerja pada sel kanker payudara MCF-7 menggunakan program ArgusLab dengan pembanding canertinib dan parameter yang diukur adalah energi bebas Gibbs ikatan (ΔG) dan konstanta inhibisi (Ki). Hasil dari penelitian ini diperoleh senyawa alkaloid golongan isokuinolin yakni 6,7-dihidro-17-hidroksierisotrin. Senyawa 6,7-dihidro-17-hidroksierisotrin diprediksi mempunyai aktivitas sitotoksik terhadap sel kanker payudara MCF-7 dengan nilai ΔG (Kcal/mol) dan Ki (nM) masing-masing sebesar -8,11457 dan 1,12.10-6. Sehingga dapat disimpulkan bahwa tumbuhan E. poeppigiana mempunyai potensi sebagai bahan dasar obat herbal antikanker payudara.
Senyawa-Senyawa Aromatik dari Ekstrak Daun dan Kulit Batang Dysoxylum parasiticum Serta Toksisitasnya Terhadap Artemia salina Mayanti, Tri; Wahyuni, Aneu; Indriyani, Indri; Darwati, Darwati; Herlina, Tati; Supratman, Unang
Chimica et Natura Acta Vol 5, No 1 (2017)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (285.604 KB) | DOI: 10.24198/cna.v5.n1.12818

Abstract

Dysoxylum parasiticum (Meliaceae) merupakan salah satu spesies tumbuhan endemik Indonesia. Beberapa spesies dari genus yang sama telah diketahui kandungan senyawa dan keaktifannya sebagai antimalaria, antitumor, antimokroba, dan antiinflamasi. Tujuan dari penelitian ini adalah untuk memperoleh nilai LC50 ekstrak n-heksana, etil asetat, metanol dari daun dan kulit batang D. parasiticum terhadap larva Artemia salina, serta struktur kimia senyawa hasil isolasi. Satu senyawa turunan flavonoid, kuersetin (1), bersama-sama dengan senyawa fenolik skopoletin (2) telah diisolasi berturut-turut dari daun dan kulit batang D. parasiticum (Meliaceae). Struktur molekul senyawa-senyawa tersebut telah ditetapkan berdasarkan data spektroskopi UV, IR, MS, dan NMR serta perbandingan terhadap data yang telah dilaporkan sebelumnya. Uji toksisitas ekstrak n-heksana, etil asetat, dan metanol dari daun D. parasiticum menunjukkan nilai LC50 berturut-turut 13,3, 37,2, dan 7 ppm. Ekstrak n-heksana, etil asetat, dan metanol dari kulit batang D. parasiticum menunjukkan nilai LC50 berturut-turut 127,9, 52,3, dan 25,2 ppm. Senyawa kuersetin (1) dan skopoletin (2) menunjukkan nilai LC50 berturut-turut sebesar 7,4 dan 18,2 ppm.
Cytotoxic Assay From Stem Bark Aglaia minahassae and Aglaia simplicifolia Against HeLa Cervical Cancer Cell Lines Kurniasih, Nunung; Milawati, Hersa; Fajar, Mohamad; Abdulah, Rizky; Putri Huspa, Desi Harneti; Supratman, Unang
Indonesian Journal of Pharmaceutical Science and Technology Suppl 1, No. 1 (2018)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (829.03 KB) | DOI: 10.15416/ijpst.v1i1.16116

Abstract

Cervical cancer ranks as the 2nd leading cause of female cancer in Indonesia. One of healing methods is chemotherapy, but this method still has many side e ects and also expensive treatment. Therefore, natural products discoveries need to be developed due to its important role as an alternative for anti- cancer drug. The aim of this research was to get IC50 value from methanol, n-hexane, ethyl acetate, and n-buthanol from stem bark of A. minahassae dan A. simplicifolia. Stem bark of A. minahassae (1.6 kg) and A. simplicifolia (1.1 kg) was grounded by methanol and its extract is successively extracted by n-hexane, ethyl acetate, and n-buthanol. Their extract’s cytotoxicity was then evaluated against HeLa cell lines. This research showed that A. minahassae’s most cytotoxic extract against HeLa cell lines was n-hexane (IC50 = 27.4190 μg/mL) and n-buthanol (IC50 = 4.3924 μg/mL). Meanwhile, A. sim- plicifolia most cytotoxic extract extract against HeLa cell lines was n-hexane (IC50 = 23.3098 μg/mL). Key words: A. minahassae, A. simplicifolia, cytotoxic assay, HeLa cell lines
Bioactivity Formulation Of Leaf Extract Of Kalanchoe pinnata And Seed Of Azadirachta indica Against Spodoptera litura Paramita, Hedi; Puspasari, Lindung Tri; Maharani, Rani; Supratman, Unang; Hidayat, Yusup; Meliansyah, Rika; Dono, Danar
CROPSAVER - Journal of Plant Protection Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

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Abstract

The aim of this research is to know the most effective concentration of liquid formulation of Kalanchoe pinnata leaf extract 50 EC and Azadirachta indica seed extract 50 EC against S. litura. The experiment was conducted at the Laboratory of Pesticides and Enviromental Toxicology, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran. The concentration were tested of each formulation were 1%, 2%, 3 %, 4%,and control.  The experiment arranged in completely randomized design (CRD) and replicated three times. The results showed that the formulation of A. indica seed extract 50 EC at concentration of 2%, 3%, and 4% effective against S. Litura larvae with mortality of 76.7%, 86.7, and 93.3%  This formulation also causing a decrease in feeding activity, weight, and lenghten the development time of S.litura larvae. Unlike the case with liquid formulations of K. pinnata 50 EC extract which was less toxic and did not show significant differences in feed intake, weight of larvae, and development time of larvae compared control treatment.                Keywords: Toxicity, mortality, growth dearragement, botanical insecticide.
Kuersetin dari Daun Erythrina poeppigiana (leguminosae) Herlina, Tati; Supratman, Unang
Jurnal Natur Indonesia Vol 17, No 1 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (76.854 KB) | DOI: 10.31258/jnat.17.1.1-4

Abstract

Erythrina plants known plants “dadap” is a higher plant that grows in tropical and subtropical regions. E. poeppigiana plants was a source of secondary metabolites, which contain flavonoids. This study aims to isolate the flavonoid compounds from the leaves of  E. poeppigiana through the stages of extraction, fraction, separation and purification. E. poeppigiana leaves powder (2.5 kg) was extracted with methanol and partitioned with n-hexane and ethyl acetate. Furthermore, the separation of ethyl acetate of E. poeppigiana leaves fraction using a combination of column chromatographic was obtained pure compound (5 mg) in the form of a yellow amorphous  solid. The chemical structure of pure compound was based on the data spectroscopy (MS, UV, IR, 1H-NMR and 13C-NMR) and identified as the compound 3,3 ‘, 4’, 5,7-pentahidroksiflavon or known as quercetin.
Aktivitas Antimalaria Daun Erythrina variegata Herlina, Tati; Supratman, Unang; Subarnas, Anas; Sutardjo, Supriyatna; Abdullah, Noor Rain
Jurnal Natur Indonesia Vol 10, No 1 (2007)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.10.1.36-41

Abstract

The leaves of Erythrina variegata (Leguminosae) used tradisional plant of an antimalarial. In the course of our continuing search for novel an antimalarial compound from Erythrina plants, the methanol extract of the leaves ofE. variegata showed significant antimalarial activity in vitro toward Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) method. The methanol extract of the leaves of E. variegata showed against bothstrains of parasite with IC50of 6.8 ?g/ml against K1 and > 60 ?g/ml against 3D7, respectively. The methanol extract of the leaves of E. variegata was separated by using bioassay-guide fractionation. The n-buthanol fraction yieldedthe most activity, exhibiting equipotency against both strains of parasite with IC50of 5.1 ?g/ml against K1 and 13.5 ?g/ml against 3D7, respectively. Furthermore, by using the antimalarial activity to follow separation, the n-buthanol fraction was separated by combination of column chromatography to yield an active compound. The active compound showed antimalarial activity against both strains of parasite used with IC50 of 4.3 ?g/ml against K1 and 23.5 ?g/ml against 3D7, respectively. Its inhibition of the resistant strain (K1) was also much better compared to its inhibition of the sensitive strain (3D7), indicated that the leaves of E. variegata to be potential as antimalarial agents, but its lower potency compared to artemisinin and chloroquin.
OPTIMASI TEKNIK WESTERN BLOT UNTUK DETEKSI EKSPRESI PROTEIN TANAMAN PADI (Oryza sativa L.) Susianti, .; Sukmana, Edi; Lesmana, Ronny; Supratman, Unang
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.722 KB) | DOI: 10.29122/jbbi.v6i2.3249

Abstract

Optimization of Western Blot Technique for Protein Expression of Rice Plant (Oryza sativa L.) Western blot (WB) technique has been widely used for analyzing protein expression and for identifying specific proteins derived from animals, plants, and microorganisms. During the use of WB, especially in agricultural studies, some difficulties are encountered such as unclear or unspecific protein bands, the presence of bubbles, and the absence of protein bands on membrane. This study aims to determine the WB conditions appropriate for the protein expression of rice plants (Oryza sativa L.). Protein from rice plant was extracted and the obtained protein lysate was then used for proteomic analysis using western blot with β-actin antibody. Our experiment showed that some optimized parameters like blocking buffers, the concentration of primary antibody and the ratio of secondary antibody determined the clarity of the results. β-actin was used as internal control that measured the success of the WB technique. Results showed that lysis process was important in determining good WB results in addition to the optimal blocking solution using a BSA of 0.2%, a primary antibody concentration of 1 μg mL–1, and a secondary antibody of 1:10,000. Optimizing techniques during extraction, incubation, and documentation facilitated good WB results.Keywords: β-actin; optimization; protein; rice plant; western blotABSTRAKTeknik western blot (WB) telah banyak digunakan untuk analisis ekspresi protein dan mengidentifikasi protein spesifik dari hewan, tumbuhan dan mikroorganisme. Dalam implementasi teknik WB, khususnya studi dalam bidang pertanian, beberapa kesulitan ditemui seperti pita protein tidak jelas, tidak spesifik, adanya gelembung, hingga tidak munculnya pita protein pada membran. Penelitian ini bertujuan untuk mengetahui kondisi WB yang tepat untuk deteksi protein tanaman padi (Oryza sativa L.). Protein tanaman padi diekstraksi, kemudian lysate protein yang didapat dianalisis dengan metode westernblot menggunakan antibody β-actin. Penelitian kami menunjukkan bahwa beberapa parameter yang dioptimasi seperti larutan blocking, konsentrasi antibodi primer dan rasio antibodi sekunder akan menentukan hasil yang jelas. β-actin digunakan sebagai kontrol internal yang menjadi tolok ukur keberhasilan teknik WB. Hasil menunjukkan bahwa proses lisis menjadi hal penting dalam menentukan hasil WB yang baik disamping larutan blocking yang optimal menggunakan BSA 0,2%, konsentrasi antibodi primer 1 µg mL–1 dan antibodi sekunder 1:10.000. Mengoptimalkan teknik selama ekstraksi, inkubasi dan dokumentasi membantu mendapatkan hasil WB yang baik.
Exercise Serum Alters Genes Related Mitochondria in Cardiomyocyte Culture Cell Lesmana, Ronny; Prasetyo, Wibowo Budi; Ray, Hamidie Ronald Daniel; Tarawan, Vita Murniati; Goenawan, Hanna; Setiawan, Iwan; Pratiwi, Yuni Susanti; Juliati, Nova Sylviana; Supratman, Unang
JURNAL PENDIDIKAN JASMANI DAN OLAHRAGA Vol 5, No 2 (2020): Improving Physical Education to Promote Healthy Growth
Publisher : Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1091.318 KB) | DOI: 10.17509/jpjo.v5i2.25547

Abstract

Exercise-induced hearth muscle adaptation is important for physiological process after exercise. This adaptation will ensure basal mitochondrial homeostasis and as a part of the mitochondria quality control. This process is reflected by equal level of biogenesis stimulation and as well as the selective degradation of old and undesirable mitochondria through fusion or fission cycle and Mitophagy. There is limited information about genetic regulation stimulated by training in cardiomyocytes. We believe there is a specific myokines or protein release in the serum and initiate cardiac muscle adaptation process. In the present study, twelve male wistar rats were appointed to two group: sedentary control and aerobic-intensity (AE, 15m/minute). Rats were trained for running with specific protocol as follows: 30 minutes/day with a 5 times/week interval for 8 weeks. On the last day, serum form control and exercise groups were taken via retro-orbital sinus. Then, 3.105  H9C2 cells (Rat cardiomyocytes cell line)  were cultured and incubated by this serum for 24 hours. After treatment, cell were extracted using trisure for RNA purification and continue with reverse transcriptase PCR. Our data showed that expression of the Pgc-1α, Mfn1, Mfn2, Opa1, Drp1, Pink, and Parkin genes were altered and modulated. Specifically, Mfn1, Mfn2, and Opa1 gene expression levels significantly increased. Interestingly, we did not find significant modulation for  Pgc-1α, Drp1, Pink, and Parkin. Taken together, serum of exercise rats might be contained with myokines or specific protein which was released during training and it altered mitochondrial genes expression in cardiomyocytes culture cell. We believe that myokines release in the serum had a contribution in cardiacmyocyte adaptation.
Co-Authors , ,. Melanie - Ruchiyat . Horizon . Susianti Ace Tatang Hidayat Ace Tatang Hidayat Achmad Zainuddin Achmad Zainuddin Ade Akbar Abdilla Ade Kania Ningsih Ade Kania Ningsih, Ade Kania Adel Zamri Agus Safari Agus Safari Agus Safari Agustini, Dewi Meliati Ahmad Darmawan Ahmad Kurniawan Ahmad Kurniawan Ahmad Ramdan Al Arofatus Naini Al Arofatus Naini Aldo Hartono Almas Widyana Alya Tsamrotul Amir M. Suruwaky Anas Subarnas Anas Urbanas Anastasya Firdausi Andi Rahim Andre A. Sonda Aneu Wahyuni Anjari, Intan Hawina Aprilia Permata Sari Aprina, Lutfia Silva Ari Hardianto Ari Hardianto Ari Widiyantoro Arif Rahman Hakim Arif Rahman Hakim Arif Rahman Hakim Arif Rahman Hakim Arlette Setiawan Arlette Setiawan Arlette Setiawan Arlette Setiawan Arlette Setiawan Arlette Suzy Puspa Pertiwi Arto Yuwono Soeroto Asep Supriadin Asri Peni Wulandari, Asri Peni Aziiz Mardanarian Rosdianto Azmi Azhari, Azmi Azmi, Mohamad Nurul Benny Joy Betry Pujiastuti C Hanny Wijaya Christina Marpaung Dadan Sumiarsa Danar Dono Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati, Darwati, Darwati Deden Indra Dinata Desi Harnet Desi Harneti Putri Huspa Desi Harneti Putri Huspa Dewa G Katja Dewa Gede Katja Dewa Gede Katja Dikdik Kurnia Dimpuulina Erna Mariati Dondin Sajuthi DUDI RUNADI Dudi Runadi Edi Suanto Edi Suanto, Edi Edi Sukmana Edi Sukmana Elis Hastuti Elisabeth Krismayanti Elvi Rusmiyanto Pancaning Wardoyo, Lisa Triyuni Arti, Mukarlina, Erina Hilmayanti Euis Julaeha Euis Julaeha Euis Julaeha Euis Julaeha Euis Julaeha Euis Julaeha Euis Julaeha Euis Julaeha Ezatul Ezleen Kamarulzaman Fadlilah, Gina Faizal Hermanto Fajar Fauzi Abdullah Fajar Fauzi Abdullah Fajar Fauzi Abdullah Fajar Fauzi Abdullah Fajar Fauzi Abdullah Fajar, Mohamad Fani Rahma Yenita Farabi, Kindi Fathurachman Fathurachman Fauzan Zein Muttaqien Ferdyan Efza Filza Yulina Ade Fizrul Indra Lubis Galuga Sinalusur Sari Ghina Izdihar Gilang Muhamad Nur Iqbal Hadi Kuncoro Hana Goenawan Hanna Goeanawan Hanna Goenawan Harizon Harizon Harlia Harlia Hasnah Osman Hasnah Osman Hedi Paramita Herdanu Rizqullah Hersa Milawati Hersa Milawati Hersa Milawati Hideo Hayashi Hideo Hayashi Hideo Hayashi Hideo Hayashi Hideo Hayashi Hideo Hayashi Hilmayanti, Erina Husein Hernadi Bahti Hutagaol, Ricson Pemimpin Ichsan Nurul Bari Ida Nur Farida Ida Nur Farida Ida Nurfarida Ihsan Rahadian Indri Indriyani Indriyani, Indri Inne Suherna Sasmita Intan Rahmayanti Iqbal Musthapa Isa Mahendra Iwan Setiawan Iwan Setiawan Iwan Setiawan Jihan Mudrika Rahmi Julia Windi Gunadi Julia Windi Gunadi Julinton Sianturi Julinton Sianturi Kadarusman Kadarusman Kansy Haikal Kansy Haikal Kautsari, Arsi Khadijah Awang Khairani, Astrid Feinisa Khalijah Awang Khalijah Awang Khalijah Awang Khalijah Awang Khalijah Awang Khalijah Awang Khlaijah Awang Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kok Tong Wong Laode Rijai Lia Destiarti Lilis Siti Aisyah Lilis Siti Aisyah Lilis Siti Aisyah Lilis Siti Aisyah, Lilis Siti Lindung Tri Puspasari Lindung Tri Puspasari M. S. Soedjanaatmadja Mantiri, Sisilia A Maryati Maryati Mas Rizky A.A. Syamsunarno Max R.J Runtuwene Max R.J Runtuwene Mayshah Purnamasari MEGANTARA, SANDRA Milawati, Hersa Moelyono Moektiwardoyo Mohamad Fajar Mohamad Fajar Mohamad Nurul Azmi Mohamad Nurul Azmi Mohamad Nurul Azmi Mohamad Nurul Azmi Mohamad Nurul Azmi bin Mohamad Taib Mohamad Nurul Azmi Mohamad Taib Mohamad Nurul Azmi Mohamad Taib Mohamed Ashraf Ali Mohd. Zaheen Hassan Mohd. Zaheen Hassan Muhammad Hanafi Muhammad Hanafi Muhammad Hanafi Muhammad Solehin Abd Ghani Muhammad Solehin Abd Ghani Murtihapsari . Mustaqim, Iqbal Wahyu Nadia Mohamed Yusoff Nadya Thufaila Nafiah, Mohamad Azlan Naini, Al Arofatus Nasrudin Nasrudin Nayla Haraswati Nayla Haraswati Noor Rain Abdullah Noor Rain Abdullah Nova Sylviana Nova Sylviana Nulelasari Nurlelasari Nunung Kurniasih Nunung Kurniasih Nunung Kurniasih, Nunung Nur Insani Amir Nur Muhammad Miftah Nurabi Ferdiana Nurlelasari Nurlelasari Nurlelasari Nurlelasari Okta Wismandanu Paramita, Hedi Ponis Tarigan Prasetyo, Wibowo Budi Pratama, Galih Bayu Prayudi Santoso Primahana, Gian Purbaya, Sari Purnama Purnama Purnama Purnama Purnamasari, Mayshah Purwo Sri Rejeki Purwoko, Agus Puspita Sari Rahmawati Rahmawati Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Ph.D Ratu Safitri Ray, Hamidie Ronald Daniel Revan Hardiawan Richa Mardianingrum Ricson Pemimpin Hutagaol Rika Meliansyah RIKA MELIANSYAH Risyandi Anwar Riyadi, Sandra Amalia Riza Apriani Rizky Abdulah Rizky Abdullah Ronauli Fitriana Ronny Lesmana Roosje Rosita Oewen Roosje Rosita Oewen, Roosje Rosita Roro Wahyudianingsih Rudi Hendra Rymond Jusuf Rumampuk S. Supriatno S. Supriatno, S. Safri Ishmayana Salam, Supriatno Sari Purbaya Sari, Aprilia Permata Satwika Nandiwardhana Setiawan Setiawan Setiawan Setiawan Setiawan Setiawan Shabarni Gaffar Shiono, Yoshihito Sianturi, Julinton Sisilia A Mantiri Siska Elisahbet Sinaga Siska Mulya Octavia Siska Mulya Octavia Siti Hani Pratiwi Sofa Fajriah Sofa Fajriah Sofa Fajriah Srikandi, Srikandi Subekti Mauluddin Sudarjat Sudarjat Sunarjati Sudigdoadi Supriatno Supriatno Salam Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriyatna Supriyatna Supriyatna Sutardjo Supriyatna Sutardjo Supriyatna Sutardjo Supriyatna Sutardjo Supriyatna Sutardjo Supriyatna, - Suseno Amien Susianti Susianti Susianti Susianti Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Teddy Budiyansyah Thaigarajan Parumasivam Tiara Prima Amalya Tjandrawati Mozef Toto Subroto Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Reksa Saputra Tri Reksa Saputra Tri Reksa Saputra, Tri Reksa Vicki Nishinarizki Vita Murniati Tarawan Vita Murniati Tarawan W.C. Taylor Wahyuni, Aneu Wawan Hermawan Wawan Hermawan Winda Sukmawati Witriany Rayapratiwi Yeni Mulyani Yenny Febriani Yun Yenny Febriani Yun Yenny Febriani Yun Yenny Febriani Yun, Yenny Febriani Yeong Keng Yoon Yofita Sandra, S.Pd., M.Pd., Zico Farlin, Dr. Budiwirman, M.Pd., Yoshihito shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yum Eryanti Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi, Yuni Susanti Yusup Hidayat