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Journal : Journal of the Indonesian Tropical Animal Agriculture

The effect of L-carnitine in Tris egg yolk-based diluent on the quality of Pasundan bull semen preserved in chilled condition Darussalam, I.; Arifiantini, R. I.; Supriatna, I.; Rasad, R. S. D.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 3 (2020): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.3.197-205

Abstract

The study was conducted to investigatethe effect of L-Carnitine supplementation in Tris-eggyolk (TEY) diluents to optimize the quality of Pasundan bull liquid semen. Semen samples were collected from three Pasundan bulls (6 – 7 years old)usinganartificial vagina. Semen samples were evaluated macroscopically and microscopically, semen having ≥70% progressive motility, ≥500×106ml-1 sperm concentration and ≤ 20% sperm abnormalities were divided into 5 equal part. Each part  wasdiluted with TEYand supplemented with0 mM(control), 1 mM, 2 mM, 3 mM and 4 mM L-Carnitine. Liquidsemen was storedat5°C.Sperm motility was evaluated every 12 h  objectively using Computer Asissted Sperm Analysis (CASA) until progressive motilityreached 40%. The best diluent was demonstratedby progressive motility (PM)by TEY supplemented with 1 mM L-Carnitine(43.08±0.49%) that remained up to 108 h compared tothecontrol group (43.63±0.70%) that remained up to 72 h (P<0.05). In conclusion, 1 mM L-Carnitine in TEY was the best concentration for preservation of Pasundan bull semen stored in the liquid form. 
Histomorphological changes, sperm quality and testosterone level after administration and cessation of Pegagan (Centella asiatica) extract in rats Solihati, N.; Purwantara, B.; Supriatna, I.; Winarto, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 3 (2018): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.3.218-229

Abstract

The objective of this study was to determine histomorphological change of seminiferous tubules, sperm quality and testosterone level after administration and cessation of pegagan extract. The research used 4x2 factorial complete randomized design, the first factor was dose-administration duration and the second factor was cessation length of pegagan extract. Parameters consisted of degree of spermatogenesis, sperm quality (motility, concentration and abnormality), and testosterone level. The data were processed using analysis of variance and Duncan’s test. Results showed that there was no interaction between dose-administration duration and cessation length. There was no significant difference of dose-administration duration of pegagan on degrees of spermatogenesis, sperm motility and testosterone level. Cessation length did not significantly affect degrees of spermatogenesis, sperm motility, and testosterone level although these results indicated that cessation of pegagan extract for 12 days increased degrees of spermatogenesis and sperm motility, indicated the antifertility potency of pegagan extract. In conclusion, Pegagan extract has potency as anti-fertility agent although the degree of spermatogenesis, sperm quality and testosterone levels were still in the normal range for the reproductive process and not irreversible, and pegagan could be explored as an reversible anti-fertility agent.