Article Per Year (5 Year)
p-Index From 2019 - 2024
2.083
P-Index
This Author published in this journals
All Journal Jurnal Kedokteran Syiah Kuala Folia Medica Indonesiana The Indonesian Biomedical Journal Indian Journal of Forensic Medicine & Toxicology Ophthalmologica Indonesiana Vision Science and Eye Health Journal Ophthalmologica Indonesiana
Komaratih, Evelyn
Department Of Ophthalmology, Faculty Of Medicine, Universitas Airlangga/Dr.Soetomo General Hospital, Jl. Mayjen. Prof. Dr. Moestopo No. 47, Surabaya
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Physics Public Health Other

Published : 12 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : The Indonesian Biomedical Journal

 1 
Matrix Metalloproteinase-3 Down Regulation and Cell Migration Inhibition in Human Pterygium Fibroblasts by Mitomycin-C, Curcumin and Fibrin Glue Ferdian Ramadhan; Jamaluddin Jamaluddin; Ismi Zuhria; Luki Indriaswati; Evelyn Komaratih
The Indonesian Biomedical Journal Vol 14, No 2 (2022)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v14i2.1825

Abstract

BACKGROUND: Pterygium is an ocular surface disease that often occurs in tropical countries with a high recurrence rate. Matrix metalloproteinase-3 (MMP-3) play a key role in the inflammatory process of pterygium. This study aims to investigate the ability of curcumin and fibrin glue (FG) in suppressing the expression of MMP-3, and whether can be expected as adjuvant therapy to reduce pterygium recurrence.METHODS: Human pterygium fibroblasts (HPF) obtained from primary cultured of pterygium were treated with no treatment, curcumin, mitomycin-C (MMC), and FG. MMP-3 expression was analyzed using immunocytochemistry and the intensity measurement was done using ImageJ software. Cell migration was measured by scratching and stratification of fibroblast culture after cell confluence, and assessed for 48 hours.RESULTS: The expression of MMP-3 were lower in the HPF treated with 100 mol/mL curcumin, 200 mol/mL, and FG (2205.84±86.1 pg/mL, 1002.51±25.22 pg/mL, 1131.55±17.71 pg/mL, respectively) in comparison with untreated HPF (4703.49±108.9 pg/mL). The expression of MMP-3 were significantly different between groups (p<0.001). Cell migration of HPF after scratching with curcumin intervention at 200 mol/mL decrease from 178.67±2.85 (24 hours) to 88.83±1.48 (48 hours). Meanwhile the migration in FG group also decrease from 180.4±2.56 (24 hours) to 72.45±1.25 (48 hours).CONCLUSION: Curcumin and FG able to reduce the expression of MMP-3 and inhibit the migration of HPF cells.KEYWORDS: curcumin, mitomycin C, fibrin glue, human pterygium fibroblast, MMP-3
Co-Authors Affannul Hakim Annisa Kinanti Asti Camilla Amanda Prakoeswa Cita Rosita Sigit Prakoeswa Diana Yuliawati Ferdian Ramadhan Herdina Ramadhani Heriyawati Heriyawati Ismi Zuhria Jamaluddin Jamaluddin Listya Dyah Rihardini Luki Indriaswati Ni Made Inten Lestari Ni Putu Ayu Reza Dhiyantari Nurwasis Nurwasis Primitasari, Yulia Suparman Suparman Susy Fatmariyanti Yulia Primitasari Yuyun Rindiastuti Yuyun Rindiastuti Yuyun Rindiastuti