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Additional calcium carbonate into concentrate diet for sheep fed ensiled king grass as a based-diet Mathius, I-W; Lubis, D; Wina, E; Nurhayati, D.P; Budiarsana, I.G.M
Indonesian Journal of Animal and Veterinary Sciences Vol 2, No 3 (1997)
Publisher : Indonesian Animal Sciences Society

In order to ascertain the effect of additional calcium carbonate into concentrate diet, on the performance of sheep fed ensiled king grass as a basal diet, a trial was conducted using 28 growing sheep ( average body weight 17 _+ 1 .4 kg). Based on body weight, the animals were grouped and randomized into four dietary treatments in block randomized design . Dietary treatments were (i) chopped king grass + 400 g of concentrate, (ii) ensiled king grass + 400 g concentrate + 0 % of calcium carbonate, (iii) ensiled king grass + 400 g concentrate + 5 % calcium carbonate and (iv) ensiled king grass + 400 g concentrate + 10 % calcium carbonate . Results showed that offering 5 % of calcium carbonate into concentrate diet increased (P < 0 .05) dry matter and nutrient intake of silage, but digestibility coefficients were similar (P > 0 .05) for all groups . No differences in the apparent digestibility of the nutrient components were observed, but crude protein decreased significantly (P < 0 .05) . A significant relationship ( P < 0 .01) was found between nitrogen intake (NI) and nitrogen retention (NR), and the equation was Â NR = - 0.1848 + 0.3788 NI ( r = 0.9 ) . Based on data found that feeding only ensiled king grass as a single diet could not meet the maintenance requirement of energy and protein, therefore, additional energy and crude protein sources is needed . Â Keywords : Silage, king grass, growing sheep

Improvement of feed efficiency using protected-energy in the ration of ewes Mathius, I-W; Yulistiani, Dwi; Wina, E; Haryanto, B; Wilson, A; Thalib, A
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 1 (2001)
Publisher : Indonesian Animal Sciences Society

High producing ewes required high amounts of energy, which can not be met through feeding conventional ration. Therefore, supplementing energy should be done, especially protected-energy. The purpose of this study was to determine the effects of additional of protected fat/energy on the consumption of ration and the performance of ewes and lambs. Thirty-four local ewes were randomly allotted to four levels of by-pass energy groups, in which parts of energy content in the concentrate was replaced with rumen-protected fatty acids in the proportion of 0 (R1), 5 (R2), 10 (R3), and 20% (R4). Consumption, nutrient digestibility and animal performance (ewes and lambs) were determined by standard procedures. Results showed that the daily dry matter intake during pregnancy (g/kg BW0.75) did not differ among diet (72.1 + 2.56). The crude fibre intake varied slightly between treatment (P>0.05), those being slightly lower intake on the highest replacement of energy content in concentrate diet (R4). Dry matter digestibility were 52.4 + 0.24, 52.1 + 0.30; 51.8 +0.28; and 51.3 + 0.81 in groups R1, R2, R3, and R4, respectively, which were non-significantly different from each other. These results confirmed that the by-pass energy sources replacements did not affect rumen fermentation. The same tendency was also found for crude protein digestibility (P>0.05) i.e. 60.1; 61.8; 61.2; and 60.2 for R1, R2, R3, and R4 respectively. The average daily gain of ewes during gestation period was affected (P<0.05) by by-pass energy replacement and the effect was linear with increasing by-pass energy replacement. Significant difference was not found in the total birth weight of lambs and among dietary treatments. Lambs growth rate during the first 8 week of lactation was the highest on ewes fed diet containing the highest amount of by-pass energy sources (R4). It can be concluded that by-pass fatty acids can be used in ewesâ ration without affecting rumen fermentation and gave a positive respons to animal performance. Â Key words: By-pass energy, ewes ration, gestation and lactating phase

Effectiveness of Aspergillus oryzae fermentation culture to improve digestion of fibrous feeds Lubis, Darwinsyah; Wina, E; Haryanto, B; Suhargiantatmo, T
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 2 (2002)
Publisher : Indonesian Animal Sciences Society

Studies regarding the use of living microorganisms as supplement have been done in the last two decades to improve digestion process in the rumen. Many factors affect the use of the supplement, such as ration composition and physiological status of the animals. Materials used in this experiment are Aspergillus oryzae (AO) derived from âtaucoâ (salty fermented soybean) and soybean sauce processing factories located in Bogor, Cianjur, and Sukabumi districts (15 sources), and also AO culture collections of the Indonesian Res. Inst. for Anim. Prod. (2 strains) and one strain from Indonesian Res. Inst. for Vet. Sci. in Bogor. The fungus was first isolated in potato dextrose agar (PDA), incubated at room temp. (26-300C) for 5 days for purification. The AO from PDA then inoculated into cooked rice enriched with mineral mix, oven-dried (40-450C), then ground and kept in refrigerator as a stock culture for further use. To produce more AO (scaling-up), the culture is inoculated in two basal media, i.e. soybean meal and âonggokâ (tapioca processing waste) enriched with mineral mix, dried and ground, then kept in refrigerator as A. oryzae fermentation culture (AOFC). Ground-dried King grass was used for in vitro digestion trials using sheep rumen fluid, which was not supplemented (control) or supplemented with the AOFC (10% w/w) from various sources. Three best AOFC (by origin) were chosen and used for further digestion study. The study was run using 2 (media) x 3 (AOFC origin) factorial experiment based on a completely randomized design and Duncanâs MRT was applied to test differences among treatment means. Preliminary results indicated of the AOFC-SP66, -F172, and -CT4 used, the best AOFC was SP66, as it increased (P<0.05) fiber (NDF) digestion (10.5% better than the control). Total VFA productions were similar, but acetate content in the rumen fluid was lowered (P<0.05), while propionate and butyrate levels were alleviated (P<0.05) by the AOFC-SP66. Ammonia content was not affected by addition of AOFC. No differences were detected on digestion parameters between the two media used for AO cultivation, therefore, it is suggested to use âonggokâ for producing AOFC in large scale. VFAs composition pattern suggests that AOFC was more suitably used for meat rather than for milk production. Key words: Fungus, King grass, digestibility, rumen fluid

Effect of tempe waste on excreation of purine derivatives and microbialâN supply in lactating Etawah crossbred goats Astuti, D.A; Wina, E
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 3 (2002)
Publisher : Indonesian Animal Sciences Society

The aim of this study was to evaluate excretion of purine derivatives and microbialâN supply in lactating Etawah crossbred goats fed with fermented soybean waste. Sixteen first lactating goats were randomly allotted into four dietary treatment groups that received 50% king grass plus R1: 50% concentrate, R2: 25% concentrate and 25% fresh tempe waste, R3: 25% concentrate and 25% fermented tempe waste, and R4: 25% concentrate and 25% gelatinizing of liquid tempe waste. Fermented tempe waste was made by fermentation of tempe waste (seed content of soybean) using Aspergillus niger, while for the gellatinizing of liquid tempe waste was made by gelatinized with maize flour. Protein balance studies were conducted during two week trial and at the end of the research. Urinary protein and purine derivatives were collected for analysis. MicrobialâN supply was calculated from purine derivatives excretion. Results showed that nitrogen consumptions were significantly different between R4 and three other treatments and apparent digestible nitrogen in R3 were higher than that of R4 (P<0.05). The nitrogen retention in R1 and R3 were higher than that of R2 and R4. Urinary purine derivatives in this study showed that allantoin, xanthine and hypoxanthine in R3 were higher than that of R4, while R1 and R2 were the same and the highest uric acid excretion and total purine derivatives were observed in R3. MicrobialâN supply were significantly different between all treatments where R3 was the highest. This research concluded that fermented soybean waste had the highest total purine derivatives excretion and microbialâN supply to the lactating Etawah crossbred goats. Â Key words: Etawah crossbred goats, Aspergillus niger, allantoin, xanthin and hypoxanthin

Feeding of Aspergillus oryzae fermentation culture (AOFC) to growing sheep: 2. Growth rate and feed efficiency Lubis, D; Haryanto, B; Wina, E; Sugiharyantatmo, T
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 4 (2002)
Publisher : Indonesian Animal Sciences Society

Utilization of yeast and or filamentous fungi as feed additive to ruminants has been of interest since the late 1980âs. Two fungi species have been commercially produced in the United States, (1) Yea-Saccï£¨ containing living cells of Saccharomyces cerevisiae and (2) Amafermï£¨ bearing Aspergillus oryzae fermentation extract. It has been demonstrated and proven that the cultures can enhance rumen development and function in young ruminants. This paper concerns the use of Aspergillus oryzae fermentation culture (AOFC) as feed additive for young-growing male âGarutâ sheep. The A. oryzae was cultured in a media made of mineral-enriched âonggokâ flour, a material of tapioca processing waste. The AOFC was prepared gradually by incubating the fungus at room temperature (26 â 300C) for 5 days, dried at 400C and ground. The AOFC was added to a commercial concentrate (GT-03) at 0, 5 and 10% (w/w) levels, as treatment C0 (control), C1, and C2, respectively. Fifteen growing âGarutâ sheep were used and the concentrate feed treatments were randomly allotted based on a randomized block design. Drinking water was available at all time. The amount of feed offered (chopped King grass and concentrates) and their refusals were weighed daily and live-weight of sheep was measured once a week in the morning. Daily feces was collected and weighed in the last 10 days of the 14-week experimental period. All feed and fecal samples were analyzed for dry matter, crude protein, total fiber (NDF), and ash. AOFC supplementation resulted in higher weight gains (P<0.05), which were 94.81; 122.08; and 140.52 g/d for C0, C1, and C2 treatments, respectively. Dry and organic matter, as well as protein intake was also significantly increased by inclusion of AOFC into concentrate diet (P<0.05). The increment in nutrient intake was from increased consumption of concentrates, and not from King grass, however, there was no effect of AOFC supplementation on feed efficiency. Â Key words: A. oryzae, sheep, gain, efficiency

Effect of processing on nutritive value of corn cobs: Chemical composition and in vitro digestibility Yulistiani, Dwi; Puastuti, W; Wina, E; ., Supriati
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 1 (2012)
Publisher : Indonesian Animal Sciences Society

One of the causes of in low productivity of ruminants in Indonesia is the lack of continual availability of high quality feed. Agriculture by-product such as corn cob is potential to be used as roughage for ruminants. However its nutrient quality is low; therefore, processing needs to be done to increase its nutrient quality.Â The objective of the study was to obtain technology which able to increase the nutritive value of corn corb. Corn cobs were ground to obtain corn grain size and then treated with one of six treatments. These treatments were (1). Urea treatment at the level 3% of DM corn cob, (2). Fermentation using Trichoderma viridae; (3). Combination of fermentation using T. viridae and urea at the level 0.5% DM, (4). Fermentation using Aspergilus niger, (5). Fermentation using A. niger and urea at the level of 0.5% DM and (6). Ensiling of corn cob. All treated samples were dried in an oven then were ground for chemical analyses and in vitro digestibility. The study was done using randomised completely design and the data was analyzed using SAS programe v6.1. Result shows that untreated corn cob had low quality nutritive value which was indicated by its low protein content (2.9%) and low in vitro digestibility (42.5%). All treatments could increase corn cobâs protein contents; the highest increase was on urea treatment and fermentation of corn cob using A. niger supplemented with urea (210 and 172% respectively). The in vitro digestibility was only significantly increased by urea treatment where its digestibility was increased by 43%. On the other hand, fermentation using A. niger either with or without urea supplementation increased neutral detergent fibre digestibility by 300% and 200%. In conclusion, nutritive value of a corn cob can be improved by urea treatment at the level of 3% or by fermentation using A. niger. Key Words: Corn Cob, Silage, Ammoniation, Fermentation

The effects of inorganic and organic Zn, Cu and Mo supplementation to the in vitro digestibility of grass ., Supriyati; Yulistiani, D; Wina, E; Hamid, H; Haryanto, B
Indonesian Journal of Animal and Veterinary Sciences Vol 5, No 1 (2000)
Publisher : Indonesian Animal Sciences Society

The effects of inorganic and organic Zn, Cu and Mo supplementation to the in vitro digestibility of Elephant grass were studied in this trial. The in vitro trial used the TILLEY dan TERRY method modified by VAN SOEST with the incubation of the grass for 48 hours in sheep rumen liquor medium. Mineral added as fonn of organic and inorganic. The treatment of mineral inorganic supplementations were by adding single e1emen Cu, Zn and Mo and its combination. Mineral added was Zn (as chloride and sulphate salts) 5 ppm, Cu (sulphate salt) 0,1 ppm dan Mo (molybdate salt) 5 ppm, with 4 replicates. Meanwhile the organic minerals added were in the proteinate fonns. Parameter measured were in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD), pH, VF A total and NH3. Statistical analysis was done by using Complete Split splitsplot design. The supplementation increased pH value, VFA total, IVDMD and IVOMD value (P<0,05) and decreased NH3 value. The highest IVDMD and IVOMD values obtained at the mixture supplementation of mineral Zn (as Zn S04), Cu and Mo, from 58,31 became 69.73% and 52.22 became 62.55% respectively for IVDMD and NOMD. pH value increased from 6.48 to 7.05 and ammonia content decreased from 1,17 to 0,14%. The organic mineral supplementation, en-proteinate dan Zn-proteinate showed that the adding of Zn, Zn and Mo, Zn and Cu also the combination of Zn, Cu and Mo, resulted in IVDMD values as 70.29; 69.97, 64.12 and 63.93%. Further more IVDMD value at the supplementation of Cu and the combination of Cu and Mo were 65.08 and 60,49%. It can be concluded that the supplementation of minerals in the form of inorganic or organic could improve the IVDMD values Â Key words: Three element, in vitro digestibility, sheep

Performa dan Profil Beberapa Komponen Darah Sapi Peranakan Ongole yang Diberi Pakan Mengandung Lerak (Sapindus rarak De Candole) D A Astuti; E Wina; B Haryanto; S Suharti
Media Peternakan Vol. 32 No. 1 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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The problem with low meat production is caused by several factors, ie: management, feed and animal health. The development of integration system such as cattle-rice, cattle-corn and cattle-palm oil aims to utilize feed from local resources. However, sometimes local feedstuff causes a problem of imbalance nutrient. There are several strategies to overcome this problem and one of them is the use of feed additives from natural substance or plant resources such as lerak (Sapindus rarak De Candole). This research was aimed to utilize lerak fruit to improve performance and evaluate some blood profiles of Ongole crossbred cattle. The in vivo work was done using 12 Ongole crossbred cattle receiving 3 different feeds as treatment, i.e. 1) feed without lerak (high roughage diet) as control, 2) feed contained 2,5% lerak, and 3) feed contained 5% lerak. Growth, daily gain, blood profiles (leucocytes, cholesterol, trigliceride and globulins) were observed. The results showed no significant differences for nutrient intake, meaning that the ration has good palatability. Average daily gain of cattle fed with 2,5% lerak was 20% higher than those with control diet. White blood cells tended to decrease caused by lerak treatments, but the globulin profiles (alpha, beta and gamma) were not significantly affected in all treatments. Blood cholesterol concentration decreased following the increase of lerak percentage. It is not recommended to use lerak in the cattle ration for more than two months because of the decreasing of leucocytes. Key words: Sapindus rarak, in vivo, leucocytes, globulins, palatability

Biological Activity of Tannins from Acacia mangium Bark Extracted by Different Solvents E Wina; Susana I W.R; B Tangendjaja
Media Peternakan Vol. 33 No. 2 (2010): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Acacia mangium bark is abundant byproduct of wood industry in Indonesia. It is underutilized and mainly used as fire wood for the wood industry. The bark contains high level of tannin but the tannin has not been extracted or produced commercially. Tannin isolate can be used for several purposes such as tanning agent for leather, adhesive for plywood or particle board, etc. In ruminant, tannin can be detrimental but can also be beneficial. This experiment was aimed of getting the highest yield of tannin extract with the highest biological activity in rumen fermentation. Nine different solvents at different temperatures were used to extract tannin from A. mangium bark. The extracts were analyzed for their tannin contents and biological activities. Tannin content was analyzed using folin ciocalteau and butanol-HCl methods. Biological activity was described as a percentage of an increase in gas production in the in vitro rumen-buffer fermentation, with and without addition of PEG. The results show that Na2SO3 solution extracted more tannin than other solutions and the higher the concentration of Na2SO3 solution, the higher the yield of tannin extract. The solution of 6% sodium sulphite gave the highest yield of tannin extract (31.2% of original bark sample) and the highest concentration of tannin (18.26%) but produced a negative effect on in vitro fermentation (% increase of gas production = 2.70%). Extraction with 50% acetone gave a high yield of extract (22.28% of original bark) which contained 12.98% of tannin and showed the highest biological response (% increase of gas production = 216%). In conclusion, sodium sulphite solution is not recommended for tannin extraction if the tannin will be used as feed additive in ruminant feed; on the other hand, the aqueous acetone (50% acetone) solution is a better choice to be used.

Microbial Population and Fermentation Characteristic in Response to Sapindus rarak Mineral Block Supplementation S Suharti; A Kurniawati; D A Astuti; E Wina
Media Peternakan Vol. 33 No. 3 (2010): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This experiment was conducted to evaluate the effect of supplementation with lerak extract combined with mineral block on protozoal and bacterial population, and fermentation characteristic in vitro. The experimental design was completely randomized block design with 3 treatments and 4 replications. Control diet was a substrate that consisted of concentrate, forage and feed block with ratio 50 : 48 : 2, respectively. The treatments as a substrate were: control diet (C), C + 0.09% lerak extract, and C + 0.18% lerak extract from the total ration. Variables observed were protozoal and bacterial population, dry matter and organic matter degradability, N-NH3 and total volatile fatty acid (VFA) concentration. Data were analyzed using analysis of variance (ANOVA). The result showed that there were no significant effect (P>0.05) for all parameter measured with lerak extract supplementation up to 0.18% in the presence of mineral block. However, supplementation of lerak extract 0.18% only slightly reduced protozoal numbers but tended to increase bacterial numbers. Dry matter and organic matter degradability and concentration of N-NH3 were similar among treatments. Volatile fatty acids profile changed which propionate tended to increase and acetate tended to decrease and ratio of acetate to propionate tended to decrease. In conclusion, addition of lerak extract up to 0.18% from total ration in the presence of mineral block was not yet effective to depress protozoal population, but could modify fermentation characteristic in vitro.

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