Ngurah Intan Wiratmini
Biology Departement, Faculty of Mathematic and Natural Science Of Udayana University

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Kadar Hormon Estrogen Dan Ketebalan Endometrium Tikus (Rattus norvegicus) Betina Ovariektomi Setelah Pemberian Ekstrak Daun Lamtoro (Leucaena leucocephala Lam. De Wit) Fernandez, Maria Antonia Margaretha; Wiratmini, Ngurah Intan; Suarni, Ni Made Rai
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p15

Abstract

Lamtoro (Leucaena leucocephala Lam. De Wit) merupakan tanaman yang digunakan sebagai obat dan pakan ternak. Lamtoro mengandung zat aktif dari golongan senyawa streroid, alkaloid, flavonoid, polifenol dan tannin. Zat aktif yang terkandung pada lamtoro bersifat fitoestrogen karena mengandung golongan senyawa steroid, alkaloid dan flavonoid. Tujuan penelitian ini untuk mengetahui pengaruh pemberian ekstrak daun lamtoro terhadap kadar hormon estrogen dan ketebalan endometrium tikus (Rattus norvegicus) betina yang telah diovariektomi. Penelitian ini menggunakan Rancangan Acak Lengkap dengan empat perlakuan yaitu K- (kontrol negatif diberi minyak jagung), K+ (kontrol positif diberi estrogen sintetik 2 mg), P1dan P2 diberi diberi ekstrak daun lamtoro sebanyak 200 mg/kg BB dan 250 mg/kg BB). Masing masing perlakuan terdiri dari 6 ulangan sehingga digunakan 24 ekor tikus betina. Ekstrak daun lamtoro diberikan secara oral sebanyak 2 ml/ekor/hari selama 15 hari. Data yang diperoleh dianalisis dengan One Way ANOVA dan dilanjutkan dengan uji Duncan Multiple Range Test (DMRT). Hasil penelitian menunjukkan pemberian ekstrak daun Lamtoro (Leucaena leucocephala Lam. De Wit) dosis 250 mg/kg BB dapat meningkatkan kadar hormon estrogen serum darah dan ketebalan endometrium tikus betina yang diovariektomi. Kata kunci: Leucaena, Ovariektomi, Estrogen, Endometrium, Tikus
GANGGUAN SPERMATOGENESIS SETELAH PEMBERIAN MONOSODIUM GLUTAMAT PADA MENCIT (MUS MUSCULUS L.) SUKMANINGSIH, A.A.SG.A; ERMAYANTI, I GUSTI AYU MANIK; WIRATMINI, NGURAH INTAN; SUDATRI , NI WAYAN
Jurnal Biologi Udayana Vol 15 No 2 (2011): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

This study was conducted to investigate the effect of monosodium glutamate (MSG) on spermatogenesis of male experimental mice.using 32 twelve-week old mice. They were divided randomly into 4 groups, each group consisted of 8 mice. One group was used as control, three groups were used as treatment, which received 1.5 mg/g body weight/day, 3 mg/g body weight/day and 4.5 mg/g body weight/day of MSG solution in destilled water for 35 days. The result showed that MSG treatment led to disturbance on spermatogenesis by reduction in some of the parameter studied. There was a significant (p < 0.05) decrease in the number of pachytene spermatocyte and spermatid 15, where as the decrease in spermatogonia was insignificant (p>0.05).
PERTUMBUHAN, HISTOPATOLOGI OVARIUM DAN FEKUNDITAS IKAN NILA MERAH (OREOCHROMIS NILOTICUS) SETELAH PAPARAN PESTISIDA ORGANOFOSFAT SETYAWATI , IRIANI; WIRATMINI, NGURAH INTAN; WIRYATNO, JOKO
Jurnal Biologi Udayana Vol 15 No 2 (2011): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Fish cultures in rice fields or areas that use water from a pesticide-polluted irrigation or river, are particularly vulnerable to the negative impact of pesticides. This study aims to assess the effect of organophosphates on growth, ovarian histopathology and fecundity of red tilapia (Oreochromis niloticus). After acclimatization, 160 fishes (fingerlink stage) were kept in four ponds sized 2x1,5x0,8 m each. This study used a complete randomized block design and organophosphate administration through the feed. The study used four groups: control, and variations in dose of 2 ml (D1), 6 ml (D2), and 10 ml (D3) of pesticide per 100 grams of pellet, which is given in the morning (treatment) and in the evening (regular feed). Data were taken at week 3, 6, and 9, then the organophosphate treatments were stopped, and the last data were taken at week-18. Statistical analysis was performed by Anova and Duncan's Multiple Range Test. The results showed that the organophosphate pesticide inhibits the growth by reducing fish length and weight, reducing ovarian weight, increasing the number of oocyte atresia, damaging structure of the ovarian histology, and decreasing fecundity by reducing the number of egg production in the ovarian of red tilapia.
THE DEVELOPMENT OF OVARIAN FOLLICLE CELLS AND CORPUS LUTEUM OF MICE (Mus musculus) SWISS WEBSTER GIVEN Leucaena leucocephala LEAF EXTRACT Wiratmini, Ngurah Intan; Sudatri, Ni Wayan; Setyawati, Iriani
International Journal of Biosciences and Biotechnology Vol 3 No 1 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

This research was conducted to determine the effect of Leucaena leucocephala leaf extract on the development of ovarian follicle cells of mice (Mus musculus). Twenty four female mice, 10 weeks old, were divided into 4 groups. The control group (P0) were given 0.9% NaCl and the treatment group P1, P2 and P3 were given 0.5, 1 and 1.5 g/ kg bw of Leucaena leaf extract, respectively. The treatments were administered daily for 15 days by gavage. After the treatment ended, all mice were dissected to collect the ovaries. Histological preparation of the ovaries used paraffin method and Hematoxylin-Eosin staining. Observations were made on the number of primary, secondary, and tertiary oocytes, and also De Graaf follicles and corpus luteum. Data were statistically analyzed using One Way ANOVA method. The results showed there were no significantly differences (P>0.05) on the number of primary, secondary, and tertiary oocytes, as well as De Graaf follicles and corpus luteum among the control and treatment groups. The mimosine content of Leucaena leaf extract was 0.87% (less than 1%) so it could not inhibit the secretion of Follicle Stimulating hormone by the pituitary gland. The doses of Leucaena leaf extract used in this research did not significantly affect the follicles development of mice ovaries. In this study, the mimosine content of Leucaena leaf extract decreased 91.8%.
THE EFFECTS OF DIETARY INCLUSION OF DETOXIFIED Leucaena leucocephala LEAF MEAL ON THYROIDAL ACTIVITY OF RATS DURING GESTATION-LACTATION PERIOD Wiratmini, Ngurah Intan; Narayani, Inna; Setiasih, Ni Luh Eka
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.757 KB) | DOI: 10.24843/IJBB.2018.v05.i02.p02

Abstract

Leucaena leucocephala provides the highest amount of proteins compared to other greens. However, its use is restricted due to the content of mimosine, an antinutrition non-protein amino acid known to be toxic to animals. This study aimed to observe the effect of administration of more than 10% of processed Leucaena leucocephala leaf meal to the level of T3, T4 and the thyroid histopathology. In this study, Leucaena leucocephala leaves were soaked in distilled water for 12 hours. The processed of Leucaena leucocephala leaf meal was made into pellets containing 0%, 7.5%, 15%, and 22.5% leaf meal of total feeds respectively, and was fed to the rats during pregnancy and lactation. The level of triiodothyronine (T3) and thyroxine (T4), and the thyroid histological features were the parameters observed. The collected data were statistically analyzed by SPSS 20.0 for Windows using One-way ANOVA followed by Duncan Multiple Range Test to observe any significant difference among the 4 treatment groups (?=5%). Meanwhile, the presence of hyperplastic cells and follicle lumens filled with vesicles and colloids was descriptively analyzed by means of available literatures. The analysis of T3 and T4 level revealed that there was no any significant difference between the control and treatment groups. The epithelial cells of thyroid follicles in the treatment groups of P1, P2 and P3 showed hyperplasia and were detached from their respective basal membranes.
EFEKTIVITAS EKSTRAK DAUN SUKUN (Artocarpus communis Forst.) DALAM MENURUNKAN KADAR GLUKOSA DARAH DAN MEMPERTAHANKAN JUMLAH SPERMA PADA TIKUS (Rattus norvegicus L.) Ari Wijana Dipa, I Putu; Sudatri, Ni Wayan; Wiratmini, Ngurah Intan
SIMBIOSIS Vol 3 No 2 (2015)
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

Breadfruit (Artocarpus communis Forst.) leaves have a high antioxidant content. This research aims to know the effect of breadfruit leaf extracts on blood sugar levels and the amount of sperm in rat. This study used a Completely Randomized Design (CRD) using 30 rats aged 2-3 months and weighing 150-200 grams. Male rats were divided into six groups, i.e. negative control group (K1) without treatment, groups K2, P1, P2, P3 and P4 are given then NaCl 0.9%, breadfruit leaf extracts 50 mg/kg, 100 mg/kg, 200 mg/kg body weight, and glibenclamide 1 mg/kg body weight respectively. The treatments were given every day for 21 days. Blood sugar levels were measured before and after aloksan administration, day 7, 14, and 21 after treatment as well as counting the number of sperm after 21 days. The data obtained were analyzed with ANOVA and Duncan Multiple Range Test (DMRT), using the program of SPSS For Windows version 22. Results of the study showed that breadfruit (Artocarpus communis Forst.) leaf extracts could lowered blood glucose levels and the most effective concentration was 100 mg/kg body weight of breadfruit leaf extracts given for 21 days with the effectiveness of 66,77%. Breadfruit leaf extracts did not inhibit spermatogenesis. So the amount of rat sperm did not different significantly among treatment. Keywords: blood sugar levels, breadfruit, rat, sperm count
PENGARUH PEMBERIAN RHODAMIN B TERHADAP SIKLUS ESTRUS MENCIT (Mus musculus L.) BETINA Febrina, G.A.A Rina; Wiratmini, Ngurah Intan; Sudatri, Ni Wayan
Jurnal Biologi Udayana Vol 17 No 1 (2013): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

The research was conducted to identify the effect of rhodamine B on estrous cycle on female mice (Mus musculus L.). Twenty-four female mice were used, and randomly divided into four groups.  Rhodamine B was given orally (gavage) with a dose of  0 ppm (control), 150 ppm, 300 ppm, and 600 ppm in two estrous cycles.  Treatments were started when mice in estrus phases. The time between first and the next estrus phase were observed from vaginal smear preparation. Data obtained were analysed with One Way Anova.  If the differences were appeared between treatments, then the LSD test was performed. The results showed that rhodamine B was significantly increased the time of estrous cycle (P<0.05).
MOTILITAS DAN VIABILITAS SPERMATOZOA MENCIT (MUS MUSCULUS L.) SETELAH PEMBERIAN EKSTRAK TEMU PUTIH (CURCUMA ZEDOARIA (BERG.) ROSCOE.) Ashfahani, Elfira Dzikri; Wiratmini, Ngurah Intan; Sukm aningsih, A.A.S.A
Jurnal Biologi Udayana Vol 14 No 1 (2010): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

This research was aimed to study the effect of temu putih (Curcuma zedoaria (Berg.) Roscoe.) extract treatment on viability and motility of mice (Mus musculus L.) spermatozoa. The extract was given orally once a day in 35 days. The animals were divided into four groups; one control group and three treatment groups with six replicates. (M0 = control group; M1 = group was given 100 mg/Kg body weight/day; M2 = group was given 200 mg/Kg body weight/day; M3 = group was given 300 mg/Kg body weight/day). The result of the study showed that motility and viability of spermatozoa were decreased significantly (p< 0.05) after receiving temu putih (C. zedoaria (Berg.) Roscoe.) extract for 35 days.
PENGARUH PEMBERIAN EKSTRAK DAUN EKOR NAGA (Rhaphidophora pinnata, Schott) TERHADAP PERKEMBANGAN UTERUS MENCIT (Mus musculus) BETINA YANG TELAH DIOVARIEKTOMI Margaretha Ferandez, Maria Antonia; Wiratmini, Ngurah Intan; Manik Ermayanti, Ni Gusti Ayu
Jurnal Biologi Udayana Vol 19 No 2 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Tanaman ekor naga (Rhaphidophora pinnata, Schott.) mengandung zat aktif dari golongan senyawa alkaloid, flavonoid, saponin, tanin dan triterpenoid/steroid. Zat aktif yang terkandung dalam tanaman ekor naga bersifat fitoestrogen. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian ekstrak daun ekor naga terhadap perkembangan uterus mencit (Mus musculus) betina yang telah diovariektomi. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari empat kelompok perlakuan dengan masing-masing enam ulangan. PerlakuanP0 sebagai kontrol diberi NaCl 0,9% dan perlakuan P1, P2 dan P3 diberi ekstrak daun ekor naga dengan dosis 50, 100  dan 150 mg/KgBB. Ekstrak daun ekor naga diberikan setiap hari secara oral selama 14 hari sebanyak 0,2 ml dengan metode gavage. Variabel yang diamati adalah bobot uterus, diameter uterus dan ketebalan endometrium. Data yang diperoleh dianalisis dengan One Way Anova dilanjutkan dengan uji Duncan Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa pemberian ekstrak daun ekor naga (R. pinnata, Schott) menyebabkan peningkatan ketebalan endometrium sebesar 38,7% dan peningkatan diameter uterus sebesar 30,3% dibanding dengan kelompok kontrol. Dosis optimal ekstrak daun ekor naga (R. pinnata, Schott) yang berpengaruh terhadap perkembangan uterus mencit adalah 100 mg/KgBB.
PANJANG SIKLUS ESTRUS MENCIT (Mus musculus L.) YANG DIBERI PEMANIS BUATAN ASPARTAM SECARA ORAL Sulastri, Sri; Wiratmini, Ngurah Intan; Suriani, Ni Luh
Jurnal Biologi Udayana Vol 18 No 2 (2014): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian aspartam terhadap panjang siklus estrus mencit betina dewasa. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 6 ulangan. Perlakuan P0 sebagai kontrol diberi aquades dan perlakuan P1, P2 dan P3 diberi aspartam dosis10 mg/kg bb; 15 mg/kg bb dan20 mg/kg bb. Aspartam diberikan setiap hari secara oral (gavage) selama 14 hari sebanyak 0,3 ml. Setelah 14 hari, apusan vagina dibuat setiap 8 jam dalam sehari selama dua kali siklus estrus. Variabel yang diamati adalah panjang waktu tiap fase dalam siklus estrus. Hasil analisis menggunakan Uji One Way Anova dan Uji Kruskal Wallis menunjukkan bahwa aspartam secara nyata (P<0,05) memperpanjang siklus estrus dengan peningkatan dosis yang diberikan.