Claim Missing Document
Check
Articles

Free Radicals Scavenging Activities of Low Molecular Weight Sodium Alginate (LMWSA) from Sargassum polycystum, Produced by Thermal Treatment Yudiati, Ervia; Pringgenies, Delianis; Djunaedi, Ali; Arifin, Zaenal; Sudaryono, Agung
Aquacultura Indonesiana Vol 19, No 1 (2018)
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (620.849 KB) | DOI: 10.21534/ai.v19i1.121

Abstract

In this study, the effects of alginate from Sargassum polycystum molecular reduction by thermal heating on DPPH anti radical scavenging activity were investigated. Raw alginate as the control treatment was heated at 140oC in a laboratory oven for different time courses 1.5, 4.5, and 7.5 hours. The assessment of molecular weight, UV-visible and FT-IR spectroscopic studies were applied. By heat treatment, molecular weight of polymer was decreased in a time-dependent manner, though there is no significant difference between 4.5 h and 7.5 h samples. The UV-visible spectroscopic studies pointed that there was a new absorption band between 250 and 290 nm in alginate heated treatments. The higher antiradical scavenging activity were reached from 1.5 h and 4.5 h treatments (19.83% and 20.07%). Interestingly, the antiradical scavenging activity of the longest heating treatment (7.5 h) was reduced (16.85%), similar to the raw alginate (17.89%). Prolonged heat treatments influenced the antioxidant activity and reduced the ability of donate electrons or hydrogen atoms to inactivate this radical action.
TRANSPLANTASI LAMUN Thalassia hemprichii DENGAN METODE JANGKAR DI PERAIRAN TELUK AWUR DAN BANDENGAN, JEPARA Wulandari, Dwi; Riniatsih, Ita; Yudiati, Ervia
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.767 KB) | DOI: 10.14710/jmr.v2i2.2347

Abstract

The seagrass bed is a coastal ecosystem which have an important role for the coastal environment. The seagrass beds are vulnerable with changes of water environmental conditions. The decrease of seagrass beds area in the world are the result from environmental stresses both of natural and impact of human activities. Transplantation is one way of to rehabilitate the condition of seagrass beds were damaged. The purpose of this research was to determine the survival rate and the rate of growth of transplanted seagrass Thalassia hemprichii with anchor method at Teluk Awur and Bandengan Waters Jepara. The method used in this research was field experimental method. Determination of sites using purposive random sampling method. The research was conducted at two stations are Teluk Awur Water as Station I and Bandengan Water as Station II. Each station divided into 3 plots of observation. The environmental parameters were taken are: salinity, temperature, current velocity, depth, nitrate, phosphate, dissolved oxygen, organic matter, composition and grain size of the substrate. The results showed that the survival rate of seagrass transplants at Teluk Awur water was higher when compared to the survival rate of seagrass transplants at Bandengan water. The survival rate of seagrass transplants at Teluk Awur water have a range of of 38,89% to 41,67%, while the survival rate of seagrass transplants at Bandengan water have a range of 20,97% to 23,15%. The average range growth rate of seagrass transplants at Teluk Awur water was 0,13 cm/day to 0,16 cm/day, while the average range growth rate of seagrass transplants at Bandengan water was 0.16 cm/day up to 0.17 cm/day.
Rasio Jenis Kelamin dan Tingkat Kematangan Gonad pada Rajungan (Portunus pelagicus) Linnaeus, 1758 (Malacostraca:Portunidae) yang Didaratkan di Kelurahan Pacar, Kabupaten Rembang Simanjuntak, Sri Devi; Yudiati, Ervia; Subagiyo, Subagiyo
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1091.42 KB) | DOI: 10.14710/jmr.v9i1.25784

Abstract

ABSTRAK : Rajungan (Portunus pelagicus) memiliki nilai ekonomi tinggi, dimana berbanding lurus dengan penangkapan yang meningkat. Tingkat pemanfaatan yang tidak mengindahkan ukuran dan kondisi rajungan dapat mempengaruhi struktur ukuran dan stok rajungan di suatu perairan. Penelitian ini bertujuan untuk melakukan kajian morfometri, rasio jenis kelamin dan tingkat kematangan gonad rajungan yang didaratkan di Kelurahan Pacar, Kabupaten Rembang. Metode yang digunakan dalam penelitian bersifat deskriptif dan eksploratif. Pengamatan rajungan dilakukan pada salah satu pengepul di Kelurahan Pacar. Rajungan yang diteliti berdasarkan morfometri, rasio seksual dan tingkat kematangan gonad selama 30 hari. Informasi ini dapat dijadikan pedoman dalam menentukan kebijakan mengenai pengelolaan perikanan rajungan yang berkelanjutan. Hasil dari penelitian diketahui bahwa ratio kelimpahan rajungan jantan sebesar 45% (1342 ekor); rajungan betina 55% (1658 ekor) dari 3000 ekor sampel rajungan. Rasio perbandingan jantan dan betina 0,80:1 berdasarkan uji chi square. Berdasarkan distribusi ukuran lebar karapas rajungan di perairan Kelurahan Pacar adalah berkisar antara 60–179 mm dan distribusi berat sebesar 23–251 gram. Distribusi tingkat kematangan gonad pada perairan Kelurahan Pacar adalah 558 ekor pada TKG 1; 761 ekor pada TKG 2; dan 339 pada TKG 3. Data menunjukkan bahwa pola pertumbuhan rajungan yang ada di perairan Kelurahan Pacar adalah allometrik negatif yaitu pertumbuhan lebar karapas lebih cepat bertambah daripada berat tubuhnya baik rajungan jantan maupun rajungan betina.ABSTRACT : Blue swimming crab (Portunus pelagicus) has a high economic value, which is causing the crab catch to rise as well. The catch rate that does not consider the size and maturity of sea crab can affect the size structure and population stock of sea crab in waters. This research to study morphometry, sexual ratio and maturity level of the gonads landed in Pacar Village, Rembang Regency.The method used in this study descriptive and exsplorative. Observation of blue swimming crab samples was carried out in one of the collectors in the village. Blue swimming crab was observed based on morphometry and sexual ratio for 30 days. The results of the study revealed that the abundance of male blue swimming crab was 45% (1342 male blue swimming crabs) and the abundance of the female blue swimming crabs was 55%  (1658 female blue swimming crabs) of 3000 blue swimming crab samples. The ratio of male and female   0.80 ; 1 based on the chi square. Based on the size distribution of the width of the blue swimming crab carapace in the waters of Pacar Village it range from 60-179 mm and the distribution of weight 23-251 grams. The distribution of gonad maturity level in the waters Pacar Village is 558 female blue swimming crab at TKG 1; 761 female blue swimming crab at TKG 2; and 339 female blue swimming crab at TKG 3. Data is showed that the blue swimming crab growth pattern in Pacar Village waters was negative allometric both male and female blue swimming crab.
KAJIAN HUBUNGAN FOSFAT AIR DAN FOSFAT SEDIMEN TERHADAP PERTUMBUHAN LAMUN Thalassia hemprichii DI PERAIRAN TELUK AWUR DAN PULAU PANJANG JEPARA Setiawan, Dedi; Riniatsih, Ita; Yudiati, Ervia
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.386 KB) | DOI: 10.14710/jmr.v2i2.2349

Abstract

Seagrass growth is limited by the supply of nutrients such as nitrogen and phosphate particulates that serve as energy for photosynthesis. The magnitude of the role of phosphate in the metabolism and growth of seagrass Thalassia hemprichii important to serve as a study to determine the relationship of nutrients to the growing rate of seagrass in Teluk Awur and Pulau Panjang Jepara. The purpose of this study was to determine the concentration of phosphate in the water and sediment in the waters of the Teluk Awur and Pulau Panjang and its relation to seagrass leaf growth rate of T. hemprichii. The study was conducted in April - July 2012 includes literature studies, site surveys, field data collection and analysis of samples. Analysis of the relationship phosphate concentration on the rate of growth of seagrass T. hemprichii using bivariate correlation analysis. The results showed that the average growth rate - the average seagrass highest type T. hemprichii found in Teluk Awur on 8 week (0.81 cm/day). Meanwhile, the lowest growth rate found in Teluk Awur at week 2 (0.14 cm/day). The content of phosphate concentration of water in the Teluk Awur ranged from 0.009 to 0.028 mg/L and in Pulau Panjang ranged from 0.012 to 0.025 mg/L. The concentration of phosphate sediments in the Teluk Awur ranged from 20.870 to 23.250 mg/kg and Pulau Panjang 25.650 to 27.190 mg/kg. Relations water phosphate concentration and phosphate sediments to seagrass leaf growth in Teluk Awur are not considered closely related ,instead phosphate water to the growing rate of seagrass in Pulau Panjang is low and phosphate sediments to seagrass growth as very closely.
Uji Toksisitas Ekstrak Kloroform Cangkang dan Duri Landak Laut (Diadema setosum) Terhadap Mortalitas Nauplius Artemia sp Aprilia, Hilda Ayu; Pringgenies, Delianis; Yudiati, Ervia
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.711 KB) | DOI: 10.14710/jmr.v1i1.890

Abstract

Eggshells and spines of sea urchins (Diadema setosum) have toxins that can lead to certain biota surrounding death. Presumably the sea urchin has toxic compounds that can affect the surrounding environment. Because it needed a way to determine the toxic properties of sea urchin. Bioactive compounds contained in a previous study showing shells and spines of certain species of sea urchin pigment is coated by a black liquid that is stable. This fluid can be used as a mesh and skin coloring. It can also be used as fertilizer. This study aims to determine the lethal toxicity (LC50-24 h) of the chloroform extract of sea urchin of mortality Nauplius Artemia sp. The method used in this study is the experimental laboratory. Chloroform extract of the eggshells and sea urchin spines were tested using animal toxicity tests Nauplius Artemia sp.The results showed that the lethal toxicity (LC50-24 h) of the Nauplius Artemia sp in the chloroform extract of sea urchin eggshells at 133.58 ppm. As for the chloroform extract of sea urchin spines obtained results for 168.178 ppm. Chloroform extracts of both eggshells and sea urchin spines have potential activity as anti-microbial bio
PENENTUAN KANDUNGAN PIGMEN FIKOBILIPROTEIN EKSTRAK Spirulina platensis DENGAN TEKNIK EKSTRAKSI BERBEDA DAN UJI TOKSISITAS METODE BSLT Farihah, Shofa; Yulianto, Bambang; Yudiati, Ervia
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.52 KB) | DOI: 10.14710/jmr.v3i2.5411

Abstract

Spirulina platensis merupakan mikroalga dengan kandungan pigmen tertinggi yaitu fikosianin. Pemanfaaatan fikosianin sebagai bahan baku industri sudah banyak digunakan. Lebih jauh lagi, keberhasilan ekstraksi pigmen fikosianin mengarah kepada potensinya sebagai antitumor, antiinflamasi, antimikroba, dan antioksidan. Tujuan dari penelitian ini adalah untuk menentukan kandungan pigmen fikobiliprotein mikroalga Spirulina platensis strain BBPBAP dan menentukan konsentrasi dan nilai LC50 ekstrak mikroalga Spirulina platensis strain BBPBAP dengan metode uji BSLT dengan teknik ekstraksi waktu freezing  yang berbeda. Metode penelitian yang digunakan adalah eksperimental laboratorium dengan analisis data secara deskriptif. Uji kandungan pigmen fikobiliprotein dilakukan dengan uji spektrofotometri dan uji toksisitas ekstrak Spirulina platensis dilakukan dengan menghitung nilai LC50-24 jam dengan metode uji BSLT (Brine Shrimp Lethality Test). Hasil uji kandungan pigmen fikobiliprotein Spirulina platensis strain BBPBAP Jepara didapatkan pigmen fikobiliprotein tertinggi yaitu pigmen allo-fikosianin serta perlakuan tanpa freezing (K) memiliki kandungan tertinggi di antara dua perlakuan lain, yaitu sebesar fikoeritrin = 28 mg/ml, fikosianin = 81 mg/ml, dan allo-fikosianin = 97 mg/ml. Hasil uji toksisitas BSLT (Brine Shrimp Lethality Test) ekstrak Spirulina platensis dengan pelarut aquades memperlihatkan bahwa nilai LC50-24 jam sebesar 0,176 ppm.
Distribusi Ukuran dan Tingkat Kematangan Gonad Portunus pelagicus, Linnaeus, 1758 (Malacostraca : Portunidae) di Perairan Rembang, Jawa Tengah Magfirani, Dina Ayu; Yudiati, Ervia; Hartati, Retno
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1086.593 KB) | DOI: 10.14710/jmr.v8i4.24853

Abstract

ABSTRAK : Rajungan merupakan komoditas ekspor bernilai ekonomis penting dan memiliki nilai komersial yang tinggi. Tingginya permintaan pasar terhadap komoditas perikanan rajungan memicu eksploitasi yang berlebihan sehingga dapat berdampak terhadap kelestarian sumber daya rajungan. Penelitian ini bertujuan untuk mengetahui distribusi ukuran, hubungan lebar dan berat, distribusi TKG, serta nisbah kelamin rajungan (Portunus pelagicus) di Perairan Gegunung Wetan, Rembang. Penelitian ini terdiri dari pengukuran panjang dan lebar karapas, pengamatan tingkat kematangan gonad rajungan betina, serta pengamatan kualitas air (salinitas, suhu, pH, kedalaman dan kecerahan). Hasil dari penelitian ini menujukkan dari 3000 ekor rajungan yang diamati diketahui distribusi lebar karapas rajungan berkisar antara 7,3 – 16  cm dengan kisaran berat sebesar 47–262 gram. Rajungan yang tertangkap memiliki pertumbuhan yang bersifat allometrik negatif, dengan nilai b sebesar 2,12 pada rajungan jantan, 1,65 pada rajungan betina. Faktor kondisinya adalah 1,1 pada jantan dan 1,02 pada betina. Sedangkan distribusi tingkat kematangan gonad rajungan betina adalah 762 ekor pada TKG 1; 700 ekor pada TKG 2; serta 388 ekor pada TKG 3.ABSTRACT : Blue swimming crab has a high economical value. The production of blue swimming crab is exported overseas, so, therefore, triggers the excessive exploitation. This will lead to have an impact related to the sustainability of natural resource. This research  aim to find out the size distribution, the relationship of carapace widht and body weight, gonad maturity stages distribution and sex ratio of blue swimming crab (P. pelagicus) at Gegunung Wetan Waters, Rembang. This consider to have a role as a tool on planning the farming and managing the blue swimming crab conservation. This research cover the length and width measurement of blue swimming crab carapace, gonad maturity stages observation of female blue swimming crab and water quality measurments (salinity, temperature, pH, water depth and water brightness). The result showed that 3000 blue swimming crabs have 7.3-16 cm of widht carapace distribution and 47-262 gram of average weight. It can be concluded that growth are negatively allometric with b value (2,12) for the male crabs and b value (1.65) for female crabs.. The condition factor of male crab  is 1,1 and female crabs is 1,02. Based on the research, the result also shows that gonad maturition stages of female crabs were 762 crabs for stage 1, 700 crabs for stage 2 and 388 crabs for stage 3.
Aktivitas Antioksidan dan Kandungan Total Fenolik Pada Teripang di Perairan Karimunjawa, Jepara Avigail, Yolanda; Yudiati, Ervia; Pringgenies, Delianis
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.442 KB) | DOI: 10.14710/jmr.v8i4.24600

Abstract

Teripang merupakan hewan jenis Echinodermata. Beberapa species teripang, misalnya H. scabra, H. fuscogilva dan T. ananas memiliki nilai jual yang tinggi. Meskipun tidak semua spesies memiliki nilai jual yang tinggi, teripang berpotensi untuk dikembangkan dalam bidang kesehatan, terutama dalam kemampuannya untuk meredam radikal bebas dan mencegah berbagai penyakit degeneratif. Tujuan dari penelitian ini yaitu untuk mengidentifikasi, menentukan aktivitas antioksidan, kandungan total fenol, serta kadar karotenoid pada ekstrak beberapa teripang yang berasal dari Perairan Karimunjawa Jepara. Hasil identifikasi dari ke empat spesies adalah Stichopus cf. quadrifasciatus, Pearsonothuria graeffei, Bohadschia vitiensis, dan Holothuria atra. Ekstraksi dilakukan dengan teknik maserasi dinding tubuh teripang menggunakan pelarut metanol. Uji aktivitas antioksidan dilakukan dengan menggunakan metode DPPH (1,1-difenil-pikrilhidrazil) sebagai agen radikal bebas. Penentuan total fenolik dilakukan menggunakan metode folin-ciocalteu, dan penentuan kadar karotenoid dilakukan dengan menggunakan spektrofotometer. Hasil menunjukkan bahwa aktivitas antioksidan Bohadschia vitiensis, Stichopus cf. quadrifasciatus, Pearsonothuria graeffei, dan Holothuria atra tergolong sangat lemah dengan nilai IC50 secara berturut-turut adalah sebesar 454,28 ± 14,4; 713,51 ± 9,1; 801,57 ± 8,4 dan 1535,47 ppm. Kandungan total fenol sebesar 21,08 ± 0,49; 14,325 ± 0,21; 14,033 ± 0,33 dan 10,67 ± 0,12 mg GAE/g sampel, dan kadar karotenoidnya sebesar 23,28 ± 0,07; 25,78 ± 0,56; 11,85 ± 0,04; dan 41,44 ± 0,008 µmol/g sampel.Kata kunci: Teripang, Antioksidan, DPPH, Fenolik, Karotenoid Sea cucumbers are sea benthos from phylum Echinoderms phylum. Some sea cucumbers such as H. scabra, H. fuscogilva, T. Ananas have a high economical price. Eventhough, some sea cucumbers have the potency of being a healthy food. It it scientifically proven that some sea cucumbers have the ability to reduce free radicals and prevent various degenerative diseases caused by the excessive free radicals. The aims of this study were to identified, determine antioxidant activity, total phenol content, and carotenoid levels in sea cucumber extracts originally from Karimunjawa waters. Some of the sea cucumbers obtained are Stichopus cf. quadrifasciatus, Pearsonothuria graeffei, Bohadschia vitiensis, and Holothuria atra. The extraction was done by macerating the body walls with methanol. The antioxidant activity test were tested using the DPPH method as free radicals, the total phenolic content tested using the folin-ciocalteu method, and determined the carotenoid levels spectrophotometrically. Based on the results, antioxidant activity of  B. vitiensis, S. cf. quadrifasciatus, P. graeffei, and H. atra were  classified as very weak with IC50 454.28 ± 14.4; 713.51 ± 9.1; 801.57 ± 8.4 dan 1535.47 ppm. Total phenolic content were 21.08 ± 0.49; 14.325 ± 0.21; 14.033 ± 0.33 dan 10.67 ± 0.12 mg GAE / g samples, while carotenoid levels were 23.28 ± 0.07; 25.78 ± 0.56; 11.85 ± 0.04; dan 41.44 ± 0.008 µmol / g sample.Keyword : Sea cucumber, Antioxidant, DPPH, Phenolic, Carotenoid  
Pengaruh Perbedaan Periode Aerasi Karbondioksida terhadap Laju Pertumbuhan dan Kadar Total Lipid pada Kultur Nannochloropsis oculata Norbawa, Puji; Yudiati, Ervia; Widianingsih, Widianingsih
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.699 KB) | DOI: 10.14710/jmr.v2i3.3126

Abstract

N. oculata is commonly used as a natural food in larviculture. Due to the fact on its highly lipid content, N. oculata is recently becoming one of a good candidate for a source of alternative energy. Microalgae utilizes CO2 during photosynthesis. This fact will lead and used this microalgaeas a carbondioxide degradator. Providing of carbon dioxide aeration is expected to increase the rate of growth of N. oculata as well as total lipid production.This research aimed to determine the effect of different periods on carbondioxide aerationon the growth rate and total lipid production in N. oculata culture.The results showed that different time on carbondioxide aeration was significantly different (P < 0.05) on the average growth rate as well as total lipid production. Carbondioxide aeration treatment for 3 minutes have the highest average growth rate which is0,574 doubling/ day. While the average growth rate at treatment aeration for 4 minutes almost equal to the control i.e. 0,484 doubling/ day and 0,462 doubling/ day. The highest percentage of total lipid production has reached in 4 minutes carbondioxide aeration treatment (80.58%-dw). Furthermore, the lipid production on 1 minute, 2 minutes aeration time and control were 65.98%-dw, 65.77%-dw and 64.98%-dw, respectively. The treatment with carbondioxide aeration for 3 min was the lowest (39.72%-dw). Based on these results it can be concluded that carbondioxide aeration for 3 minutes increased the growth rate of N. oculata. However, the incrementon growth ratewere not accompanied withthe incrementon total lipid production.
Uji Toksisitas Ekstrak Pigmen Kasar Mikroalga Spirulina platensis Dengan Metode Uji Bslt (Brine Shrimp Lethality Test) Rizkina, Rani Agustian; Yudiati, Ervia; Sedjati, Sri
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.676 KB) | DOI: 10.14710/jmr.v2i1.2051

Abstract

Spirulina platensis is a microalgae which rich of nutrients and has a potential extracts as an antioxidant, antimicrobial, antiviral, anti-inflamantory and antitumor. The aims of this research were to determine toxicity of methanol extract and crude pigment (methanol-acetone and diethyl ether fractions) of S. platensis against nauplii Artemia sp. aged 24 hour at 3rd instar as an antitumor. The aims of this research were to determine toxicity of methanol extract and crude pigment (methanol-acetone and diethyl ether fractions) of S. platensis against nauplii Artemia sp. The research was conducted in August-October 2011 at Microalgae Laboratory, Marine Station, Diponegoro University Teluk Awur Jepara. Toxicity test methanol and crude pigment extract calculated by LC50-24 hour with BSLT (Brine Shrimp Lethality Test) methodes and pigment analysis was carried out by TLC (Thin Layer Chromatography). The results of BSLT (Brine Shrimp Lethality Test) methanol extracts and crude pigment (methanol-acetone and diethyl ether fractions) showed that LC50-24 hour values of each extract methanol (446,68 ppm), crude pigment extract ethanol-acetone (134.9 ppm) and a crude fraction pigment extract diethyl ether (91.2 ppm). The crude pigment has a cytotoxic effect LC50-24 hours <1000 ppm showed that the extracts are toxic to nauplii Artemia sp. 24 hours, 3rd instar and has a potential antitumor compounds. The results of pigments identification showed contains carotenoids and chlorophylla.
Co-Authors Abidin Nur II Adha Susanto Agung Sudaryono Agus Setyawan Akbar Harahap Alfi Satriadi Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Ridlo Alifia Nabilla Putri Alim Isnansetyo Alisa Sukma Adella Amalia Rachma Fatchiyyah Ambariyanto Ambariyanto Ambariyanto Ambariyanto Amelia Cahya Angelina Angelina, Amelia Cahya Annisa Afifah Nugroho Arief Taslihan Arumning T. Fauziah Arumning Tias Fauziah Avigail, Yolanda Ayunda Ainun Nisa Bambang Yulianto Bambang Yulianto Chika Velita Anindya Yulian Chrisna A Suryono Chrisna Adhi Suryono Chrisna Adi Suryono Danang Ambar Prabowo Dea Shinta Kharisma Adziana DEDI SETIAWAN Dedi Setiawan Delianis Pringgenies Delianis Pringgenies Delianis Pringgenies Desrina Desrina Diah Permata Wijayanti Diah Tri OktaviyantI Dieng Widawati Dina Ayu Magfirani Dinny Anjang Sari Diyah Putri Ambarwati Djoko Suprapto Dwi Wulandari Dwi Wulandari Edi Wibowo Endang Sri Susilo Endang Sri Susilo Endang Supriyantini Eny Heriyati Fadhliyah Idris Fanny Iriany Ginzel Gunawan Widi Santosa Gunawan Widi Santosa H. Endrawati Haeruddin Haeruddin Hidayati, Jelita Rahma Hilda Ayu Aprilia Hilda Ayu Aprilia Hotnida Junita Situmeang Insafitri, I Ipanna Enggar Irpan Hasibuan Irwani Irwani Ita Karlina Ita Riniatsih Ita Widowati Johannes Hutabarat Langkah Sembiring Lilik Maslukah Linggar Dirgantara Prasetyo Magfirani, Dina Ayu Mila Safitri Rizfa Mila Safitri Rizfa Mostafa Imhmed Ighwerb Muhammad Salauddin Ramadhan Djarod Muhammad Salauddin Ramadhan Djarod Muhammad Syaifudien Bahry Muhammad Syaifudien Bahry Natasya Erdza Aulia Nuril Azhar Nuril Azhar Nuril Azhar Patria Ami Wijaya Puji Norbawa Putri Hutari Girsang Rabia Alghazeer Rabia Alghazeer Rabia Alghazeer Raden Bagus Sugio Sumanta Rani Agustian Rani Agustian Rizkina Rani Agustian Rizkina Retno Hartati Ria Azizah T.N. Ria Azizah Tri Nuraeni Rini Widiyadmi Rudhi Pribadi Rustadi Rustadi Saniyatul Ulya Sari, Alfianisa Permata Sarjito - Sarjito . Shofa Farihah Simanjuntak, Sri Devi Sri Devi Simanjuntak Sri Sedjati Sri Sedjati Sri Sedjati Sri Sejati Subagiyo Subagiyo Subagiyo Subagiyo Sunarsih Sunarsih Sunaryo Sunaryo Suryono Suryono Suryono Suryono Suryono Suryono The Michael Febrian Wijaya Tika Retnowati Triyanto Triyanto Valentina R. Iriani Widianingsih Widianingsih Widianingsih Widianingsih Yolanda Avigail Yulius Docang Casessar Zaenal Arifin Zaenal Arifin Zaenal Arifin Zaneb M. Ben Mansur