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PEMANFAATAN LIMBAH CAIR SAGU UNTUK MEMPRODUKSI SELULOSA BAKTERI Ahmad, Sitti Wirdhana; Yanti, Nur Arfa; Muhiddin, Nurhayani H.
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3763

Abstract

ABSTRACTBacterial cellulose is an exopolysaccharide produced by bacteria and has a high purity level compared to plant cellulose and has unique structural and mechanical characteristics that can be utilized for various industrial purposes such as food, medical, plastic and paper. This study aims to determine the potential of sago liquid waste as a substrate for producing biocellulose and sugar concentration is required in producing bacterial cellulose from sago liquid waste. Production of bacterial cellulose from sago liquid waste was done with static condition for 14 days with treatment of sugar concentration 5,10,15 and 20% (w/v) using Acetobacter xylinum. Parameters were measured include of thickness, yield, crude fiber content and moisture content. Production of bacterial cellulose using sago liquid waste requires the addition of sugar as much as 10% with a thickness of 21.73 mm, yield of 34.97%, crude fiber of 4.5% and moisture content of 91.35%. Therefore, sago liquid waste is potentially used as a substrate for producing bacterial cellulose.  Keywords :Biocellulose, nata, Acetobacter xylinum, Production substrate  
SCREENING BAKTERI AMILOLITIK DAN SELULOLITIK DARI LIMBAH SAGU (Screening of Amylolytic and Cellulolytic Bacteria From Sago waste) Yanti, Nur Arfa; Munir, Asmawati
Jurnal BioWallacea Vol 1, No 1 (2014)
Publisher : Jurnal BioWallacea

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Abstract

Screening of indigenous bacteria from sago waste based on amylolytic and cellulolytic activity was done to obtain bacterial isolate having double activity, i.e. could to hydrolize of starch (amylolytic) and cellulose (cellulolytic). Screening amylolytic and cellulolytic bacteria was done based on amylolytic and cellulolytic activity on agar media. Determination of amylolytic activity on starch agar media was based on the presence of clear zone around the bacterial colony upon flooding with lugol’s iodine solution. Cellulolytic activity was determine based on the presence of clear zone around the bacterial colony on Carboxy methyl cellulose (CMC) agar upon flooding with congo red solution. Presence of a clear zone around the colony indicated starch and cellulose hydrolysis. The diameters of clear zone produced on CMC and starch agar were measured and used as an indication of the amylolytic and cellulolytic activities of the bacteria. The results of the screening based on amylolytic and cellulolytic activity showed that a number of 21 bacterial isolates that having both activities. LCA2 was the bacterial isolate with the highest amylolytic and cellulolytic activity as revealed by the size of clearing zone on both types of agar plates. The diameters of clear zone on starch and CMC agar were 4,98   and 3,65 cm2, respectively. Therefore, LCA2 isolate was bacterial isolate that potent for biconvertion sago hampas into value-added products. Keywords : Bacteria, Amylolytic, Cellulolytic, Sago waste.
A Study on Production of Poly-β-Hydroxybutyrate Bioplastic from Sago Starch by Indigenous Amylolytic Bacteria Yanti, Nur Arfa; Sembiring, Langkah; Margino, Sebastian; Muhiddin, Nurhayani H.
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.882 KB)

Abstract

Bacillus sp. PSA10 and Bacillus sp. PPK5 were two indigenous strain amylolytic bacteria from SoutheastSulawesi that have ability to produce bioplastic poly-β-hydroxybutyrate (PHB) from sago starch. The study wasattempted to determine the mechanism of PHB production by bacteria amylolytic was grown on sago starchcontainingmedia. Two amylolytic bacteria i.e. Bacillus sp. PSA10 and Bacillus sp. PPK5 was grown for 168 hin a mineral salts medium with sago starch as carbon source. Growth of amylolytic bacteria was monitoredby cell dry weight. Extraction of PHB was done by N-hexane acetone-diethyl ether method and PHB contentwas quantifi ed with UV spectrophotometer at 235 nm. Glucose level was determined by using kit of glucoseGOD 10” and was quantifi ed with spectrophotometer at 500 nm. Sago starch concentration was determinedby phenol method using specthrophotometer at 490 nm. The result of the study showed that Bacillus sp.PSA10 was produced PHB up to 66,81 % (g PHB/g cell dry weight) at 48 h and Bacillus sp. PPK5 up to 24,83% (g PHB/g cell dry weight) at 84 h. Bacillus sp. PSA10 has ability to converse sago starch to be PHB directlywithout glucose accumulation in the media, whereas Bacillus sp. PPK5 have to accumulate glucose as productof sago starch hydrolysis to produce of PHB. PHB synthesis by Bacillus sp. PHB production on sago starchof the Bacillus sp. PSA10 was found to be growth-associated whereas Bacillus sp. PPK5 was found to be nongrowth-associated. Therefore, two indigenous amylolytic bacteria were having of difference in biosynthesismechanism of PHB in sago starch medium and their characteristics of PHB synthesis should be consideredin developing cultivation methods for the effi cient production of PHB.Keywords : Production, PHB, Amylolytic bacteria, Sago starch.
Production of Poly--hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10 Yanti, Nur Arfa; Sembiring, Langkah; Margino, Sebastian
Indonesian Journal of Biotechnology Vol 14, No 1 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.322 KB)

Abstract

A new bacterial strain that produces amylase and poly-a-hidroxybutyrate (PHB) using sago starch as carbon source was characterized and identified to be member of the Bacillus megaterium group based on phenotypic characteristics  and 16S rDNA gene sequences. Amylase activity was determined spectrophotometrically on the basis of substrate concentration reduction. PHB production was quantified with UV spectrophotometer. The polymer produced by B. megaterium PSA10 was identified by  Fourier Transform Infrared spectroscopy (FTIR). The result of the study showed that the amylase specific activity B. megaterium PSA10 was 593,61 DUN/mg protein and PHB production from sago starch was 52,28 % of cell dry weight (CDW). FTIR analysis of the polymer indicated that the strain B.megaterium PSA10 was a potent PHB producer.
Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10 Yanti, Nur Arfa; Sembiring, Langkah; Margino, Sebastian
Indonesian Journal of Biotechnology Vol 11, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.322 KB)

Abstract

A new bacterial strain that produces amylase and poly-α-hidroxybutyrate (PHB) using sago starch as carbon source was characterized and identified to be member of the Bacillus megaterium group based on phenotypic characteristics and 16S rDNA gene sequences. Amylase activity was determined spectrophotometrically on the basis of substrate concentration reduction. PHB production was quantified with UV spectrophotometer. The polymer produced by B. megaterium PSA10 was identified by Fourier Transform Infrared spectroscopy (FTIR). The result of the study showed that the amylase specific activity B. megaterium PSA10 was 593,61 DUN/mg protein and PHB production from sago starch was 52,28 % of cell dry weight (CDW). FTIR analysis of the polymer indicated that the strain B.megaterium PSA10 was a potent PHB producer.
Optimasi Produksi Poli-β-Hidroksibutirat (PHB) oleh Bacillus sp. PSA10 Yanti, Nur Arfa; Margino, Sebastian; Sembiring, Langkah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (469.574 KB) | DOI: 10.24002/biota.v15i3.2587

Abstract

A new strain characterized as Bacillus sp. PSA10 was found to produce poly-β-hydroxybutyrate (PHB) at concentration of 52.28% (g PHB/g dry cell weight) in shaken flask culture, using sago starch as a carbon source. This research is aimed to determine the optimum culture condition of PHB production Bacillus sp. PSA10 at laboratory scale. Optimization of PHB production was conducted in this research, in terms of inoculum concentration, concentration of the major components in minimal medium, environmental condition and incubation time. The result showed that optimum conditions for the production of PHB by Bacillus sp. PSA10 were achieved at minimal medium (Ramsay medium) with 5% (v/v) inoculum concentration, 2% (w/v) sago starch, 1.0 g/l (NH4)2SO4, 6.7 g/l Na2HPO4.7H2O, and 0 g/l KCl. The optimum environmental conditions were achieved with initial pH 7, temperature 37oC, agitation speed at 150 rotary per minute (rpm) and the best of incubation time was 48 hour. Under this optimum condition, the maximum PHB production by Bacillus sp. PSA10 increased from 52.28% to 71.35% (g PHB/g dry cell weight) at 48 hour cultivation. Therefore, Bacillus sp. PSA10 is potential to apply for PHB production from sago starch at industrial scale.
SCREENING BAKTERI AMILOLITIK DAN SELULOLITIK DARI LIMBAH SAGU (Screening of Amylolytic and Cellulolytic Bacteria From Sago waste) Yanti, Nur Arfa
REZ PUBLICA Vol 1, No 1 (2015): March - May
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/rzp.v1i1.627

Abstract

ABSTRACTScreening of indigenous bacteria from sago waste based on amylolytic and cellulolytic activitywas done to obtain bacterial isolate having double activity, i.e. could to hydrolize of starch(amylolytic) and cellulose (cellulolytic). Screening amylolytic and cellulolytic bacteria wasdone based on amylolytic and cellulolytic activity on agar media. Determination of amylolyticactivity on starch agar media was based on the presence of clear zone around the bacterialcolony upon flooding with lugol’s iodine solution. Cellulolytic activity was determine based onthe presence of clear zone around the bacterial colony on Carboxy methyl cellulose (CMC)agar upon flooding with congo red solution. Presence of a clear zone around the colonyindicated starch and cellulose hydrolysis. The diameters of clear zone produced on CMC andstarch agar were measured and used as an indication of the amylolytic and cellulolyticactivities of the bacteria. The results of the screening based on amylolytic and cellulolyticactivity showed that a number of 21 bacterial isolates that having both activities. LCA2 was thebacterial isolate with the highest amylolytic and cellulolytic activity as revealed by the size ofclearing zone on both types of agar plates. The diameters of clear zone on starch and CMCagar were 4,98 and 3,65 cm2, respectively. Therefore, LCA2 isolate was bacterial isolate thatpotent for biconvertion sago hampas into value-added products
ANALISIS BAKTERI KOLIFORM DAN PATOGEN DEPOT AIR MINUM KECAMATAN MANDONGA KOTA KENDARI Aswan, Muhammad Aswan; Darlian, Lili; Yanti, Nur Arfa
Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017 Vol 1, No 1 (2017): Seminar Nasional Riset Kuantitatif Terapan 2017
Publisher : Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (907.434 KB)

Abstract

Refill drinking water depot used primarily in sub-district community Mandonga. Which is indicative of contamination of drinking water refill that contamination of coliform bacterial and pathogenic bacteial. The aim of  the study was to determine the microbiological quality of drinking water refill in District Mandonga. In this work, carry out were analyzed technique quantitatively and qualitatively by testing the Presumtive test, Confirmed test and Complied test as well as using specific media. The result suggest that, there is a negative one, namely coliform bacterial from the village and there are four depots Anggilowu positive coliform bacterial that is depot in the village of Alolama, Wawombalata, Labibia and Korumba, with the highest MPN value found in the depot Korumba and Wawombalata is > 1100 MPN/100 mL, whereas in the Alolama village is 240 MPN / 100 mL and in the Wawombalata village  is 460 MPN/ 100 mL to MPN a value. The result of indicate that bacterial amplifier contamintaion of the depot is a non-faecal colifom bacteria (Enterobacter). Based on the test result found pathogenic bacteria Vibrio sp. at the depot in the village Alolama, Wawombalata and Labibia.Keywords­— Coliform Bacterial, Pathogen, The Quality of Drinking Water Depots
Screening of Acetic Acid Bacteria from Pineapple Waste for Bacterial Cellulose Production using Sago Liquid Waste Yanti, Nur Arfa; Ahmad, Sitti Wirdhana; Ambardini, Sri; Muhiddin, Nurhayani Haji; Sulaiman, La Ode Iman
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10241

Abstract

Bacterial cellulose is a biopolymer produced by fermentation process with the help of bacteria. It has numerous applications in industrial sector with its characteristic as a biodegradable and nontoxic compound in nature. The potential application of BC is limited by its production costs, because BC is produced from expensive culture media. The use of cheap carbon and nutrient sources such as sago liquid waste is an interesting strategy to overcome this limitation. The objective of this study was to obtain the AAB strain that capable to produce bacterial cellulose from sago liquid waste. Isolation of AAB strains was conducted using CARR media and the screening of BC production was performed on Hestrin-Schramm (HS) media with glucose as a carbon source. The strains of AAB then were evaluated for their cellulose-producing capability using sago liquid waste as a substrate. Thirteen strains of AAB producing BC were isolated from pineapple waste (pineapple core and peel) and seven of them were capable to produce BC using sago liquid waste substrate. One of the AAB strains produced a relatively high BC, i.e. isolate LKN6. The result of morphological and biochemical test was proven that the bacteria was Acetobacter xylinum. The result of this study showed that A. xylinum LKN6 can produce a high yield of BC, therefore this strain is potentially useful for its utilization as a starter in bacterial cellulose production.
EKSPLORASI BAKTERI TERMOHALOFILIK POTENSIAL PENGHASIL L-ASPARAGINASE SEBAGAI ANTIKANKER DI SUMBER AIR PANAS WAWOLESEA Jamaluddin, Jamaluddin; Alfin, Alfin; Muzuni, Muzuni; Yanti, Nur Arfa
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 5, No 1 (2018): Sains & Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (806.59 KB) | DOI: 10.33772/biowallacea.v5i1.4591

Abstract

ABSTRAK Tujuan penelitian ini adalah untuk memperoleh isolat bakteri termohalofilik penghasil enzim L-aparaginase dari sumber air panas Wawolesea. Bakteri termohalofilik penghasil L-asparaginase asal sumber air panas Wawolesea diperoleh dengan tahapan: survey dan pengukuran parameter lingkungan sumber air panas Wawolesea; isolasi bakteri pada media NA (Nutrient Agar) dan seleksi bakteri penghasil enzim L-asparaginase pada media M-9. Hasil isolasi menunjukkan adanya 52 isolat bakteri termohalofilik dan 14 isolat diantaranya mampu menghasilkan L-asparaginase. Kata Kunci: Bakteri Termohalofilik, L-Asparaginase, Sumber Air Panas Wawolesea ABSTRACT  The objective of this study was to obtain isolates of thermohalophilic bacteria producing L-asparaginase enzyme from Wawolesea hot spring. L-asparaginase producing thermohalophilic bacteria from the Wawolesea hot spring are obtained by steps; survey and environmental parameters measurement of Wawolesea hot spring, isolation of bacteria on NA (Nutrient agar) medium and selection of L-asparaginase producing bacteria on M-9 medium. The isolation results showed 52 isolates of thermohalophilic bacteria and 14 isolates of them capable of producing L-asparaginase. Keywords: Thermohalophilic bacteria, L-asparaginase, Wawolesea hot spring