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All Journal Journal of Indonesian Dental Association Jurnal Kedokteran Gigi Terpadu

Putriany Agustin, Tri

Departemen Ilmu Kedokteran Gigi Anak Fakultas Kedokteran Gigi Universitas Trisakti

Author-ID : 499351

Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology

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The Impact of Academic Potential Test (APT) on GPA Score in Bachelor Dental Program in Trisakti University Rahmi Amtha; Tri Erri Astoeti; Tri Putriany Agustin; Yohana Yusra; Wiwiek Poedjiastoeti; Indrayadi Gunardi
Journal of Indonesian Dental Association Vol 2 No 2 (2019): October
Publisher : Indonesian Dental Association

Introduction: Academic potential test (APT) is the terminology used for requirement in student recruitment process at Faculty of Dentistry, Trisakti University. According to literature, APT score is believed to predict the academic achievement in graduate school. Objectives: This study assessed the impact of APT score on GPA score at Bachelor Dental Program year 2015-2018. Methods: Cross sectional descriptive analysis were done on 486 students, divided into 4 groups based on credit semester (CS); group A 144 CS, B 100 CS, C 60 CS, and D 20 CS. Result: mean APT score were 598 + 95.46 and GPA score were 2.63 + 0.72. Linear regression test showed significant correlation between APT and GPA scores (p=0.007; r2=1.5%). It shows that APT contributed as only a little aspect for the success of students in their study. In dentistry, there are other factors that may influence GPA score beside APT such as intellectual quality, talent, behavior, psychomotor and capability in social, science and communication. Conclusion: Academic potential test could be used to predict the GPA score in Bachelor Dental Program.

Overview of the Total Bacteria and Number of Streptococcus mutans in the Saliva of Children with High Caries Activity Natasya Putri Hardini Yanis; Tri Putriany Agustin
Journal of Indonesian Dental Association Vol 3 No 1 (2020): April
Publisher : Indonesian Dental Association

Introduction: Microorganisms play a crucial role in the early stage of caries. Streptococcus mutans produces lactic acid, which enhances the risk of caries. Polymerase chain reaction (PCR) is a molecular biology technique used in the amplification and replication of DNA. PCR exhibits high specificity and a rapid detection time, as well as differentiation between microorganisms. Based on a previous study, there is a significant correlation between S. mutans in saliva and the prevalence of caries. Objectives: To provide an overview of the total bacteria and number of S. mutans in the saliva of children aged between 5 and 10 years at the Department of Pediatric, Faculty of Dentistry, Trisakti University, Jakarta, Indonesia. Methods: Saliva sample from children (n = 28, aged 5-10 years old) was collected from healthy children. 2-3 mL of a saliva samples were collected and cultured by using TYS media for total bacterial count. The number of S. mutans in the saliva of these children was counted by real-time PCR methods by using a fluorescence chemical compound green SYBR using 16S rRNA gene specific primers for S. mutans. Results: The average number of total bacterial colony count in saliva was 9.965 ± 2.078 log10 CFU/mL, and the average number of S. mutans in the saliva of children was 8.303 ± 2.351 log10 CFU/mL. Conclusion: The number of S. mutans in the saliva of children in Department of Pediatric, Faculty of Dentistry, Trisakti University was categorized is in the high-risk.

Antibiofilm Effect of Clitoria ternatea Flower Juice on Porphyromonas gingivalis in vitro Armelia Sari Widyarman; Stephani Sumadi; Tri Putriany Agustin
Journal of Indonesian Dental Association Vol 1 No 1 (2018): October
Publisher : Indonesian Dental Association

Introduction: Clitoria ternatea flower contains flavonoid such as anthocyanin that gives the blue color to its flower and has antimicrobial activity. Objectives: The aim of this study was to examine the effect of flower juice of Clitoria ternatea against Porphyromonas gingivalis biofilm viability in vitro. Methods: This study was experimental laboratory research using biofilm assay method. P. gingivalis was cultured in BHI broth in 37°C for 24h under anaerobic condition. Fresh flowers of Clitoria ternatea were extracted using mortar and pestle and diluted into 6 different concentrations: 100%, 50%, 25%, 12.5%, 6.25%, and 3.125% with phosphate buffer saline (PBS). Chlorhexidine (0.2%) was used as positive control and biofilm without treatment as negative control. The flower juice was distributed into 96 well-plates that contained biofilm of P. gingivalis and incubated for 1h, 3h, 6h, and 24h in 37°C, anaerobic atmosphere. Biofilm was measured using crystal violet dye with microplate reader (490 nm). Data were statistically analysed using one-way ANOVA test and Post Hoc test with p<0.05 was set as significant different. Result: Result showed that Clitoria ternatea flower juice significantly reduced the Porphyromonas gingivalis biofilm viability in all concentration and all incubation time. The most effective concentration to inhibit Porphyromonas gingivalis biofilm was 100% in 1h incubation time which biofilm was diminished (Optical Density=0.00). One way ANOVA test and Post Hoc test showed a significant biofilm reduction in all concentration and all incubation time after treatment with the flower juice compared to control (p<0.05). Conclusion: Clitoria ternatea flower juice has antibiofilm effect against Porphyromonas gingivalis. This result showed this flower juice may be useful for combating periodontal pathogens. However, further studies using other bacteria are still needed to confirm this result.

Co-Authors Anneke Ramadhanty Putri Wardani Armelia Sari Widyarman Hani Yulia Ningsih Indrayadi Gunardi Jeddy Jeddy Natasya Putri Hardini Yanis Rahmi Amtha Stephani Sumadi Tri Erri Astoeti Wiwiek Poedjiastoeti, Wiwiek Yohana Yusra

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