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Effect of Electron-Beam Irradiation on the Growth Profile and Fatty Acid Composition of Botryococcus sp. Halimatul, Heli Siti; Yuniarsih, Windra; Wulandari, Asri Peni
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14891

Abstract

Botryococcus sp. is an economically important microalgae as it contains carbohydrates, lipids, proteins, and a number of phytochemicals. The high concentrations of saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) in this strain also a valuable source for production of biofuel. Mutagenic engineering using irradiation has been known to increase the content of these biomass. The aim of this study is to know the effect of electron-beam irradiation to the growth profile and fatty acid composition of Botryococcus sp. In this study, Botryococcus sp. adjusted an optical density 1.0 after being exposed to different doses of electron-beam irradiation (160 kGy, 240 kGy, and 320 kGy) and induced random mutagenesis for strain improvement was observed based on high energy (1.5 MeV and 2 mA). Several mutants obtained were designated as strain B160, B240, and B320, respectively. The profile growth was significantly different between control (0 kGy) and irradiated microalgae strain. The highest growth is found in B320 mutant. Fatty acid of Botryococcus sp. control produced 7 fatty acids, B160 produced 7 fatty acids, B240 produced 6 fatty acids, whereas B320 produced 9 fatty acids. B320 produced hydrocarbon and phtalic acid as well as fatty acids. Total SFAs and MUFAs of B240 was increased 1.6 times compared to the wild type. The results would give some implications to improve the quality of biodiesel from Botryococcus sp.
Potensi Antibakteri Jamur Endofit dari Batang dan Daun Jamblang (Syzygium cumini L.) Indrawati, Ida; Ningsih, Nur Hasna; Andayaningsih, Poniah; Wulandari, Asri Peni; Rahayuningsih, Sri Redjeki
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 16, No 1 (2019): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.948 KB)

Abstract

Endophytic Fungi is one of the natural antibacterial sources that has not been studied. Jamblang (Syzygium cumini L.) is a plant of the Myrtaceae family that has many benefits for health. Endophytic shoots located on the leaves and stems of Jamblang can be one of the natural sources of antibacterial. This study was conducted to obtain endophytic fungi isolates from Jamblang stems and leaves, that have antibacterial activity against Bacillus cereus. The method used at the stage of endophytic shell isolation from Jamblang stem and leaves is Direct Dilution Plate method. At the antibacterial test stage of Bacillus cereus was used agar block diffusion method. Observations were made of the clear zone formed. Isolate Endophytic Fungi which has a clear zone that is D2 Yellow isolate, D4 White Ash, D4 gray Ash, Black B2, B2 White and B3 Yellow. The largest clear zone is shown by B2 Black isolate with a diameter of 14.75 mm.
Effect of Electron-Beam Irradiation on the Growth Profile and Fatty Acid Composition of Botryococcus sp. Yuniarsih, Windra; Halimatul, Heli Siti; Wulandari, Asri Peni
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14891

Abstract

Botryococcus sp. is an economically important microalgae as it contains carbohydrates, lipids, proteins, and a number of phytochemicals. The high concentrations of saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) in this strain also a valuable source for production of biofuel. Mutagenic engineering using irradiation has been known to increase the content of these biomass. The aim of this study is to know the effect of electron-beam irradiation to the growth profile and fatty acid composition of Botryococcus sp. In this study, Botryococcus sp. adjusted an optical density 1.0 after being exposed to different doses of electron-beam irradiation (160 kGy, 240 kGy, and 320 kGy) and induced random mutagenesis for strain improvement was observed based on high energy (1.5 MeV and 2 mA). Several mutants obtained were designated as strain B160, B240, and B320, respectively. The profile growth was significantly different between control (0 kGy) and irradiated microalgae strain. The highest growth is found in B320 mutant. Fatty acid of Botryococcus sp. control produced 7 fatty acids, B160 produced 7 fatty acids, B240 produced 6 fatty acids, whereas B320 produced 9 fatty acids. B320 produced hydrocarbon and phtalic acid as well as fatty acids. Total SFAs and MUFAs of B240 was increased 1.6 times compared to the wild type. The results would give some implications to improve the quality of biodiesel from Botryococcus sp.