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All Journal Vegetalika Jurnal Planta Tropika BIO-EDU: Jurnal Pendidikan Biologi Prosiding Seminar Nasional Program Pengabdian Masyarakat
Innaka Ageng Rineksane
Universitas Muhammadiyah Yogyakarta
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Education Medicine & Pharmacology Social Sciences

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Journal : Jurnal Planta Tropika

 1 
Pencapaian Fase Embriosomatik Manggis (Garcinia mangostana L.) dengan Penambahan Thidiazuron dalam Medium Setengah MS Cair Rineksane, Innaka Ageng
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 4, No 1 (2016)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2016.053.25-31

Abstract

One of the problems related to the establishment of mangosteen plantation is to obtain seedlings throughout the year, which can be solved by micropropagation.   The propagation of Mangosteen was done through somatic embryogenesis. The objective of this study was to determine the effect of Thidiazuron concentration in ½ MS medium to achieve somatic embryo stages of mangosteen seed. The study consisted of two experiments. Experiment 1: The embryogenic callus c.a.1 g derived from MS solid medium containing of Thidiazuron (0,1; 0,5; dan 1 mg/l) and 2,4-Dichlorophenoxy acetic acid (4,6,8 dan 10 mg/l) was subcultured into ½ MS0 liquid medium. 2). The embryogenic callus from the previous medium were subcultured into ½ MS liquid medium containing Thidiazuron (0, 1, 2, 4 dan 8 mg/L) and Casein hydrolysate 500 mg/L. The results showed that cell suspension were developed after mangosteen embryogenic callus subcultured into ½ MS0 liquid medium.  Somatic embryo stages such as globular, heart and torpedo were formed after the calli derived from the previous ½ MS solid medium containing 10 mg/L 2,4-D and 1 mg/L Thidiazuron were subcultured into ½ MS0 liquid medium in 8 weeks.  Cell suspension in ½ MS liquid medium with or without the addition of  Thidiazuron and Casein hydrolysate has induced the formation of embryo somatic stages such as globular, heart and torpedo after 6 months of incubation.
The Combination of Rice Water and BAP Enhances the Multiplication of Grammatophyllum speciosum Rineksane, Innaka Ageng; Nafiah, Siti Safitri; Dewi, Sukuriyati Susilo
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 6, No 2 (2018)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2018.085.92-99

Abstract

Grammatophyllum speciosum is the largest orchid species which lives epiphytically on the trees in the forest. The rate of natural propagation of Grammatophyllum speciosum is very slow. The objective of the research was to determine the effect and the best concentration of rice water and BAP for the multiplication of Grammatophyllum speciosum shoots. The research was a single factor experiment arranged in Completely Randomized Design with several types of multiplication media as treatments. The treatments were MS + 0.5 mg/l BAP, ½ MS + 25% rice water + 0.5 mg/l BAP, ½ MS + 50% rice water + 0.5 mg/l BAP, ½ MS + 75% rice water + 0.5 mg/l BAP, ½ MS + 100% rice water + 0.5 mg/l BAP, MS + 1 mg/l BAP, ½ MS + 25% rice water + 1 mg/l BAP, ½ MS + 50% rice water + 1 mg/l BAP, ½ MS + 75% rice water + 1 mg/l BAP, and ½ MS + 100% rice water + 1 mg/l BAP. Each treatment was replicated ten times. Variables observed in this study were survival rate, browning rate, contamination rate, the percentage of explants sprouting, the increase of shoot height, number of shoots and leaves, the percentage of rooted explants and the number of roots. The data were analyzed by using Analysis of Variance at α=5%. The results showed that various concentrations of rice water and BAP were able to multiply the shoots and affect the shoot growth and root length of Grammatophyllum speciosum. The combination of 75% rice water + 1 mg/l BAP on ½ MS medium was the best treatment for Grammatophyllum speciosum multiplication
Sterilisasi dan Induksi Daun Muda Durian (Durio zibethinus) Dalam Medium MS Dengan Penambahan Kinetin dan IAA Secara In Vitro Supangkat, Gatot; Rineksane, Innaka Ageng; Pamuji, Kurniawati
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 1, No 1 (2005)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v1i1.3110

Abstract

A research  to study the sterilization   method  and application   of Kinetin  and IAA to induce the Durian  young  leaf (Durio zibethinus) in MS  medium   was conducted in Balai Benih Induk Hortikultura in Salaman  Magelang  district  of Central  Java  started  on September  until December 2003. The Laboratory experiment   was arranged  in two phases,  which were  the optimation  phase of sterilization   and  induction   phase.  At  the  first  phase,  the  sterilization method  used  was  the modification   of Mulya  (2001) method.  The modification   use of sterilant,  vitamin  C antioxidant, Alcohol  70 %, Benlate, Agrept,  Tween-20  and Betadine  were done to obtain  effectiveness   of the sterilization.  Explants  planted  then in MS medium  for two weeks. Contamination   time, percentage of contamination   and viabilitas  (percentage of living explants)  were observed  then.  At the second phase,  the treatments were arranged  in a 3 x 3 factorial  completely   randomized   design  (CRD)  to observed  the influence  of Kinetin  and IAA combination.   The concentration   of Kinetin  observed were 2, 4, and 6 mg/I, where  as the IAA concentration   were 0.5,  1.0, and  1.5 mg/I. All treatments were  repeated  three  times,  with three samples  on each  replication.   The percentage   of browning explants, percentage  of contaminated   explants,  site of  contamination   and percentage of explants live were observed  at the end of incubation. The results  showed that sterilization  of Durian young leaves explants  with 1  g/l deterjent  for 15 minutes  then by 2 g/l Benlate  and Agrept  for 10 minutes,  then by 1  g/200 mg Vitamin C, then by Alcohol  70 % for 1  minute, then by 20% Clorox,  then by 2 drip of Tween-20  for 10 minute and then by Betadine  decreased  the contamination down to 50 %, and this kind of sterilization  was relatively better than  the other  kinds.  Application   of growth  regulators   were  not  able  to induce  explants growth,  but stimulated  callus formation  at the cutting surface though,  in the application  of Kinetin 4 mg/1 + IAA 0,5 mg/I, Kinetin 4 mg/1 + IAA  1,5 mg/1, Kinetin  6 mg/I+  IAA 0,5  mg/1 and Kinetin 6 mg/l+IAA   1,0 mg/I.
Pencapaian Fase Embriosomatik Manggis (Garcinia mangostana L.) dengan Penambahan Thidiazuron dalam Medium Setengah MS Cair Rineksane, Innaka Ageng
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 4, No 1 (2016)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2016.053.25-31

Abstract

One of the problems related to the establishment of mangosteen plantation is to obtain seedlings throughout the year, which can be solved by micropropagation.   The propagation of Mangosteen was done through somatic embryogenesis. The objective of this study was to determine the effect of Thidiazuron concentration in ½ MS medium to achieve somatic embryo stages of mangosteen seed. The study consisted of two experiments. Experiment 1: The embryogenic callus c.a.1 g derived from MS solid medium containing of Thidiazuron (0,1; 0,5; dan 1 mg/l) and 2,4-Dichlorophenoxy acetic acid (4,6,8 dan 10 mg/l) was subcultured into ½ MS0 liquid medium. 2). The embryogenic callus from the previous medium were subcultured into ½ MS liquid medium containing Thidiazuron (0, 1, 2, 4 dan 8 mg/L) and Casein hydrolysate 500 mg/L. The results showed that cell suspension were developed after mangosteen embryogenic callus subcultured into ½ MS0 liquid medium.  Somatic embryo stages such as globular, heart and torpedo were formed after the calli derived from the previous ½ MS solid medium containing 10 mg/L 2,4-D and 1 mg/L Thidiazuron were subcultured into ½ MS0 liquid medium in 8 weeks.  Cell suspension in ½ MS liquid medium with or without the addition of  Thidiazuron and Casein hydrolysate has induced the formation of embryo somatic stages such as globular, heart and torpedo after 6 months of incubation.
Pengaruh Berbagai Macam Pendinginan dan Pengemasan Terhadap Umur Simpan Jagung Manis (Zea mays saccharata) Rustiningsih Rustiningsih; Nafi Ananda Utama; Innaka Ageng Rineksane
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 1, No 2 (2005)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v1i2.3118

Abstract

This  research was aimed to study  the influence  of   kinds of cooling and packing  on the storage life of sweetcorn in order to obtain the simple postharvest  technology and easy to practice.  The research was done during the February - March of 2005 in the Research Laboratory of Agriculture Faculty, Muhammadiyah  University of Yogyakarta.  The reducing sugar analysis was done in the  Agriculture Technology Faculty of Gadjah Mada University. The laboratory  experiment  was arranged  ini 2 x 3 factorial  completely  randomized design with three replications.  The cooling first factor  was refrigerated in refrigerator to be compared  to the hydro-cooling.  Meanwhile,  the polypropilene and polyethylene  packaging were tested and compared to the unpackaged one. The flavor; reducing sugar, water content, percentage  of nonconsumable  part of sweetcorn,   fresh  weight  of ears,  and organoleptic properties  were observed. The result showed  that there was no significantly   interaction between cooling and packaging on  the  storage life  and  quality  of  sweet corn. The  cooling treatment was significantly influenced the starch and reducing sugar level  as well as the packaging treatment. Refrigeration   storage could significantly keep   the  level   of reducing  sugar  to remain higher than the hydro-cooling, this treatment  was also   decreased the starch  content, decreased the proportion  of unconsumable  part,  and maintained   the flavor. The  polyethylene    packaging significantly keep the level  of reducing  sugar to remain higher and relatively maintained the flavor   than  the polypropilene packaging or  unpackaged, but  decreased  the portion  of unconsumable  part. 
The Combination of Rice Water and BAP Enhances the Multiplication of Grammatophyllum speciosum Innaka Ageng Rineksane; Siti Safitri Nafiah; Sukuriyati Susilo Dewi
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 6, No 2 (2018)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2018.085.92-99

Abstract

Grammatophyllum speciosum is the largest orchid species which lives epiphytically on the trees in the forest. The rate of natural propagation of Grammatophyllum speciosum is very slow. The objective of the research was to determine the effect and the best concentration of rice water and BAP for the multiplication of Grammatophyllum speciosum shoots. The research was a single factor experiment arranged in Completely Randomized Design with several types of multiplication media as treatments. The treatments were MS + 0.5 mg/l BAP, ½ MS + 25% rice water + 0.5 mg/l BAP, ½ MS + 50% rice water + 0.5 mg/l BAP, ½ MS + 75% rice water + 0.5 mg/l BAP, ½ MS + 100% rice water + 0.5 mg/l BAP, MS + 1 mg/l BAP, ½ MS + 25% rice water + 1 mg/l BAP, ½ MS + 50% rice water + 1 mg/l BAP, ½ MS + 75% rice water + 1 mg/l BAP, and ½ MS + 100% rice water + 1 mg/l BAP. Each treatment was replicated ten times. Variables observed in this study were survival rate, browning rate, contamination rate, the percentage of explants sprouting, the increase of shoot height, number of shoots and leaves, the percentage of rooted explants and the number of roots. The data were analyzed by using Analysis of Variance at α=5%. The results showed that various concentrations of rice water and BAP were able to multiply the shoots and affect the shoot growth and root length of Grammatophyllum speciosum. The combination of 75% rice water + 1 mg/l BAP on ½ MS medium was the best treatment for Grammatophyllum speciosum multiplication
Co-Authors Alawiyah, Riffa Leshia Muhvi Nur Aris, Irfan Arya Eka Pranata Ayuningtias, Nandini Dewi, Sukuriyati Susilo Etty Handayani Faqikh Gesang Pramula Genesiska Genesiska Handayani, Etty Irsyadi, Muhammad Burhanuddin Jamhari Jamhari Kristianto Nugroho Muhammad Rizal Alfaris Nafiah, Siti Safitri Pamuji, Kurniawati Permatasari, Fany Puji Lestari Rani Agustina Wulandari Rerenstradika Tizar Terryana Rizki Eka Putri Rustiningsih Rustiningsih Shanti Wardaningsih Siti Nur Aisyah Siti Safitri Nafiah Supangkat, Gatot Utama, Nafi Ananda