Article Per Year (5 Year)
p-Index From 2019 - 2024
0.23
P-Index
This Author published in this journals
All Journal The Journal of Experimental Life Sciences (JELS)
Sapti Puspitarini
Study Program of Sains Education, Faculty of Mathematics and Natural Sciences, State University of Surabaya, Surabaya, Indonesia
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : The Journal of Experimental Life Sciences (JELS)

 1 
Piper betle L. Leaves Extract Potentially Reduce the Nitric Oxide Production on LPS-Induced RAW 264.7 Cell Lines Dinia Rizqi Dwijayanti; Sapti Puspitarini; Nashi Widodo
The Journal of Experimental Life Science Vol. 13 No. 2 (2023)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.02.02

Abstract

Chronic inflammation can lead to several diseases that represent the leading causes of mortality worldwide. Conventional treatment of inflammation can carry some risks. Therefore, research on herbal medicine that are suspected of having anti-inflammatory effects, such as Piper betle L., is important. This study aims to investigate the effect of P. betle L. extract on nitric oxide as a pro-inflammatory mediator. The dried leaves of P. betle L. were extracted by ethanol. RAW 264.7 cells were treated with LPS and P. betle L. extract (PBE). Nitric oxide was measured by the Griess method. Antioxidant activity was determined using 2,2–Diphenyl-1-picrylhydrazyl (DPPH) method. Total flavonoids and phenolic content were also identified by aluminium chloride and Folin–Ciocalteu colorimetric assay, respectively. This study demonstrated that the PBE has excellent NO suppression activity with the IC50 56.22±16.41 μg.mL-1, without cytotoxicity. PBE also has DPPH inhibitory concentration IC50 values of 279.67±11.36 ppm. Interestingly, PBE has a flavonoid content of 50.17±3.14 mg QE.g-1 and phenolic content of 128.92±1.2 mg GAE.g-1. These compounds are thought to be responsible for its anti-inflammatory as well as antioxidant. This study proved that P. betle L. leaves extract could be used as a candidate for anti-inflammatory drugs. Nevertheless, further research about the biological activity mechanism and their bioactive compounds' purification is still required. Keywords: Inflammation, lipopolysaccharide, Macrophage, Nitric Oxide, Piper betle. 
Co-Authors Dinia Rizqi Dwijayanti Nashi Widodo