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All Journal Biosaintifika: Journal of Biology & Biology Education
Kusbiyanto Kusbiyanto
Faculty of Biology, Universitas Jenderal Soedirman, Indonesia
Agriculture, Biological Sciences & Forestry

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Journal : Biosaintifika: Journal of Biology

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Barcoding of Fin Clip Samples Revealed High Exploitation of Plectropomus leopardus in Spermonde Archipelago Agus Nuryanto; Hendro Pramono; Kusbiyanto Kusbiyanto; Muhammad Iqbal Ghifari; Novi Andareswari
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.16142

Abstract

Spermonde Archipelago in South Sulawesi is among groupers sources in Indonesia for International Marine Live Trade. It was not much known which species are mostly exploited in those areas during the study because only fin clip samples could be obtained. Here we performed DNA forensic analysis to groupers fin clips collected from Spermonde Archipelago using barcode marker to know what species were exploited and which ones was the most exploited species. A total of 110 fin clip samples were obtained during the field trips. Sequencing of the cytochrome c oxidase 1 gene was successful for only 36 samples. Species determination was based on sequences similarity to conspecific sequence available in barcoding of life database (BOLD). BOLD similarity test placed the 36 fin clip samples into four different species, namely Cromileptes altivelis, Epinephelus ongus, Plectropomus leopardus, and Plectropomus maculatus. The K-2P taxonomic tree showed clear separation among species which was supported by high K2P genetic distances among species. Most of fin clips were identified as Plectropomus leopardus indicates that this species was the most exploited in Spermonde Archipelago. This proved that molecular identification not only can be used to biodiversity study in certain area but also in forensic analysis of a threatened wildlife. This study has important contribution for conservation effort of those groupers species, especially P. leopradus and provides new approach for taxonomist and conservation managers to identify samples, especially when only incomplete specimens are available.
Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy) from Single Spawning Agus Nuryanto; Nael Huda Qonita; Hendro Pramono; Kusbiyanto Kusbiyanto; Petrus Hary Tjahja Soedibja
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9301

Abstract

Soang gourami fingerling shows variable body sizes eventhough resulted from single spawning. Differences in body sizes among individuals is assumed to be correlated to their genetic component which can be studied using cytochrome b gene PCR-RFLP marker. This study aimed to determine specific PCR-RFLP marker among different sizes of soang gourami collected from single spawning. Genomic DNA was isolated using Chelex method. Cytochrome b gene were amplified and digested using four restriction enzymes. Specific markers were analyzed descriptivelly based on DNA band pattern appear in agarose gel. The result showed that PCR-RFLP markers of Cytochrome b-HinfI of 315 bp, and 210 bp, and also Cytochrome b-AluI of 334 bp and 189 bp are specific markers for large individuals, whereas small individuals are characterized by having Cytochrome b- HinfI 366 bp, and 159 bp and Cytochrome b-AluI 525 bp fragments. It is observed that genetic variation of Cytochrome b-HinfI and -AluI markers are possitively correlated to body size in soang gourami fingerling. Therefore, both cytochrome b-HinfI and -AluI gene can be reffered as specific markers to differentiate among different sizes of soang gourami strain fingerling from single spawning. This result proved that genetic divergences among individuals can be related with certain quantitative characters, such size related. Therefore our study can contribute on fisheries development, especially by providing new technique for fingerling selection to obtain high quality fingerling and also provide new insight the application of molecular technique in fisheries. 
Co-Authors Agus Nuryanto Hendro Pramono Muhammad Iqbal Ghifari Nael Huda Qonita Novi Andareswari Petrus Hary Tjahja Soedibja