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Journal : Majalah Kedokteran Gigi Indonesia

Pengaruh Ekstrak Etanolik Kulit Batang Jambu Mete (Anacardium occidentale Linn) sebagai Bahan Kumur terhadap Daya Perlekatan C. Albicans pada Plat Resin Akrilik H. Harsini
Majalah Kedokteran Gigi Indonesia Vol 18, No 2 (2011): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4613.302 KB) | DOI: 10.22146/majkedgiind.15398

Abstract

Latar Belakang: Candia albicans adalah mikroorganisme yang berkoloni melekat pada permukaan gigi maupun gigi tiruan. Obat kumur mengandung bahan tarapeutik yang berfungsi sebagai antibakteri. Kulit batang jambu mete antara lain mengandung senyawa fenolik yang dapat berkhasiat sebagai antibakteri. Tujuan penelitian: Untuk mengetahui apakah ekstrak etanolik kulit batang jambu mete sebagai bahan kumur berpengaruh terhadap gaya perlekatan C. Albicans pada plat resin akrilik. Metode penelitian: Ekstrak kulit batang jambu mete diperoleh dengan menggunakan metode maserasi dan menggunakan etanol sebagai pelarut. Bahan kumur ekstrak kulit batang jambu mete dibuat dengan komposisi bahan kumur standar dengan menambahkan ekstrak etanolik kulit batang jambu mete dengan konsentrasi 1%, 2%, 3%, 4%, dan 5% sebagai agen antibakteri. Penelitian dilakukan dengan menggunakan plat resin akrilik yang dibuat bentuk disk dengan ukuran diameter 10 mm dan tebal 2 mm sebanyak 24 buah, yang dibagi dalam 6 kelompok yaitu 5 kelompok perlakuan dengan menggunakan bahan kumur yang mengandung ekstrak kulit batang jambu mete dan 1 kelompok kontrol menggunakan bahan kumur standar. Seluruh plat resin dimasukkan dalam tabung C. Albicans 10 CFU/ml selama 5 menit, kemudian plat diambil dan dimasukkan dalam larutan bahan kumur standar sebagai kontrol dan larutan bahan kumur yang mengandung ekstrak kulit batang jambu mete untuk kelompok perlakuan selama 3 menit dan digetarkan. Cairan kemudian diambil sebanyak 0,1 ml dan ditanam pada piring petri dengan agar saboruraud dan diinkubasi selama 48 jam. Perhitungan koloni dilakukan menggunakan counter. Hasil penelitian: Hasil daya perlekatan pada bahan kumur standar 1912,50±14,93; pada bahan kumur dengan ekstrak 1% = 1757,50±20,16; 2% = 1335±17,08; 3% = 1220; 4% = 915±22,17 dan 5% = 670,00±38,37. Analisis varian satu jalur memperlihatkan pengaruh yang bermakna ekstrak etanolik kulit batang jambu mete terhadap daya lekat C. Albicans pada plat resin akrilik (p<0,05). Hasil LSD menunjukkan bahwa terdapat perbedaan yang bermakna antar seluruh kelompok perlakuan (p<0,05). Kesimpulan: Ekstrak etanolik kulit batang jambu mete sebagai bahan kumur berpengaruh terhadap daya perlekatan C. Albicans pada plat resin aklirik. Background: C. Albicans is a microorganism which colonized on the tooth or denture prosthesis surfaces. Mouthwashes usually contain therapeutic agent as antibacterial. The bark of Annacardium occidentale contains fenolic as antibacterial activity. The aim of this research was to determine the influence of the etanolic ectract of anacardium occidentale bark as mouthwashes on C. Albicans adherence. Method: the extract of anacardium occidentale bark was conducted in maceration method and used ethanol as solvent. Mouthwashes were made in standart composition and added annacardium occidentale bark extract 1%, 2%, 3%, 4% and 5% as antibacterial agent. As a negative control was used standart mouthwashes without extract. The research used 24 resin acrylic which made in disk shape with diameter 10 mm. This acrylic were divided in 6 group, there were 5 group treated with mouthwash that contain extract anacardium occidentale bark and 1 group treated with standart mouthwashes. All of resins plate were incubated in C. Albicans solution for 5 minute. After that resin acrylic plate were immersed in standart mouthwashes as a control and mouthwashes with anaracium occidentale bark extract and vibrate for 3 minutes. The solution then taken 0,1 ml and planted in petry dish with saboruraud agar and incubated for 48 hours. Result: Attachment of candida alvicans was: 1912,50±14,93 as control and mouthwash with extract were 1% = 1757,50±20,16; 2% = 1335±17,08; 3% = 1220; 4% = 915±22,17 and 5% = 670,00±38,37. Analyzed with one way Anova showed that the extract of annaracium occidentale bark as mouthwash influenced the cancida albicans adherence on resin acrylic surface (p<0,05). LSD analyzed showed there were significant differenced between all groups (p<0,05). Conclusion: The extract of anacardium occidentale bark extract as mouthwash were influence of the C. Albicans adherence on resin acrylic surface.
Aktivitas antibakteri ekstrak etanolik kulit batang jambu mete (Anacardium occidentale Linn.) terhadap Staphylococcus aureus H. Harsini
Majalah Kedokteran Gigi Indonesia Vol 3, No 3 (2017): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (296.563 KB) | DOI: 10.22146/majkedgiind.17498

Abstract

Antibacterial activity of cashew stembark (Anacardium occidentale Linn) on Staphylococcus aureus. Microbial activity acts as a sign of disruption of bacterial growth. The bark of cashew (Anacardium occidentale Linn.) contains phenolic compounds such as flavonoids, tannins and anacardic acid which have an activity as antimicrobial. One of the Gram positivebacteria in the oral cavity was Staphylococcus aureus (S. aureus). The aim of the study was to observe the bacterial activity of ethanolic extract of the cashew to a metal ion, i.e. Ca2+ and K+ leakage from S aureus. This research used one ose bacteria S. aureus at a density of 106 standard Brown as much as 10 mL and centrifuged at a speed of 3500 rpm for 20 minutes. The filtrate discarded, pellets in the tube was washed using phosphate buffer pH 7.0. Furthermore the ethanolic extract of the bark of the cashew stembark was added in the concentrations of 3.5% and 7% without any extract as a control, each of which was in 5 tubes, incubated in an incubator for 24 hours. The suspension was centrifused with a speed of 3500 rpm for 20 minutes prior to be filtered. Supernatant liquid was taken and measured absorbance using AAS. Data were analyzed using one way Anova p = 0.05. The results showed that leakage of Ca2+ was  at concentrations of 0%, 3%, 5% and 7% were 2.42 ± 0.82; 32.87 ± 1.97; 49.10 ± 3.33; 66.73 ± 3.29, respectively while for the K+ metal was 15.28 ± 0.46; 606.36 ± 14.14; 895 ± 9.5; 1251 ± 11.54. Anova one way showed a significant effect (p <0.050) ethanolic extract of the bark of cashew against leakage of metal ions Ca2+ and K+ at S aureus bacteria. LSD test showed a significant difference among all treatment groups. It was concluded that there was antibacterial activity of ethanolic extract of the cashew stembark on bacteria S. aureus based on leakage of metal ions Ca2+ and K+ The highest leakage of metal ions was at the concentrations of 7%. ABSTRAKAktivitas antibakteri merupakan tanda terganggunya pertumbuhan bakteri. Kulit batang tanaman jambu mete (Anacardium occidentale Linn.) mengandung senyawa fenolik seperti flavonoid dan tanin serta asam anakardat yang memiliki aktivitas sebagai antibakteri. Salah satu bakteri gram positif dalam mulut yang patogen adalah Staphylococcus aureus (S.aureus). Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri kulit batang jambu mete (anacardium occidentale Linn.) terhadap S.aureus yang ditandai dengan kebocoran ion logam. Penelitian menggunakan sebanyak 1 ose dengan kepadatan 106  CFL/mL disentrifuse dengan kecepatan 3500 rpm selama 20 menit. Filtrat dibuang, pellet dalam tabung dicuci menggunakan buffer fosfat pH 7,0. Ekstrak etanolik kulit batang jambu mete konsentrasi 3; 5 dan 7% serta tanpa ekstrak sebagai kontrol, masing-masing dalam 5 tabung, diinkubasi dalam inkubator goyang selama 24 jam. Suspensi kemudian disentrifuse dengan kecepatan 3500 rpm selama 20 menit lalu disaring. Cairan supernatan diambil diukur absorbansinya menggunakan AAS (Atomic absorption Spectroscopy). Data dianalisis menggunakan Anava satu jalur. Hasil menunjukkan kebocoran Ca2+ pada konsentrasi 0, 3, 5 dan 7% berturut-turut adalah 2,42 ± 0,82; 32,87 ± 1,97; 49,10 ± 3,3; 66,73 ± 3,29, sedangkan logam K+ adalah 15,28 ± 0,46; 606,36 ± 14,14; 895 ± 9,5; 1251 ± 11,54. Hasil analisis statistik Anava menunjukkan terdapat aktivitas antibakteri ekstrak etanolik kulit batang jambu mete. Hasil LSD menunjukkan terdapat perbedaan yang bermakna antar seluruh kelompok perlakuan. Kesimpulan terdapat aktivitas antibakteri ekstrak etanolik kulit batang jambu mete terhadap S.aureus dilihat dari kebocoran ion logam Ca2+ and K+. Kebocoran tertinggi pada konsentrasi ekstrak 7%. 
Surface roughness and colour changes of nanofilled composite resin after immersion in yogurt drink Sindy Widyaningrum; Purwanto Agustiono; Harsini Harsini
Majalah Kedokteran Gigi Indonesia Vol 6, No 3 (2020): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.41479

Abstract

Nanofilled composite resin is one of restorative materials with some weaknesses, such as changes of colour and surface roughness. These weaknesses are attributed to some factors, including frequent consumption some beverages, including yoghurt drinks. This study aims to determine the effect of long immersion of nanofilled composite resin in guava yogurt drink on discoloration and changes in surface roughness. Subjects of the study were nanofilled composite resin materials (3M Filtek Z350XT shade A3) in cylindrical shape with a diameter of 10 mm and 2 mm thickness. Study was conducted by immersing nanofilled resin composites in 10 mL of guava yogurt drink at 37 °C. Twelve specimens were divided into 3 groups with immersion duration of 12 hours, 24 hours, and 36 hours. Surface roughness measurements were carried out using a profilometer Starrett SR300 surface roughness tester, while the colour measurement parameters according to the system L * a * b * was measured using chromameter Konica Minolta CR-400 before and after treatment. Data of changes in surface roughness and colour changes data (ΔE) were calculated and analysed using one-way ANOVA. The results showed insignificant surface roughness changes but significant colour changes in nanofilled composite resin in the groups of 12 hours, 24 hours, and 36 hours. It is concluded that immersion duration in guava yoghurt drink significantly affects the colour of nanofilled composite resin.