Gingival wound healing is complex because it is located in an open area, often contaminated and exposed to various types of bacteria in the oral cavity. Histologically, wound healing shows an increase in the number of fibroblasts. Belimbing wuluh (Aver-rhoa bilimbi Linn) can be used as a wound medicine because it contains saponins that have the ability to clean and are antisep-tic; tannins and flavonoids are anti-inflammatory. This study aims to determine the increase in the number of fibroblasts in rat gingival wound healing by topically administering belimbing wuluh leaf extract with different concentrations. The study was conducted with a pretest-posttest control group design, consisting of a control group with distilled water and a treatment group with topical administration of BWL ethanol extract with concentrations of 20% and 40%. The results of the study based on the comparison test between the three groups with one-way Anova showed that the mean number of fibroblasts in the three groups after treatment was significantly different (p=0.001). It was concluded that there was an increase in the number of fibroblasts in the gingival wound healing of rats given BWL extract although at 20% concentration, the increase was higher than at 40%.