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All Journal AGROLAND: The Agricultural Sciences Journal
Hawalina Hawalina
Study Program of Agrotechnology Faculty of Agriculture, Tadulako University, Palu
Agriculture, Biological Sciences & Forestry

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Journal : AGROLAND: The Agricultural Sciences Journal

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The Growth of Kiwi Shoot (Actinidia deliciosa) on Various Kinds of Gelling Agents Mardiana Mardiana; Zainuddin Zainuddin; Mahfudz Mahfudz; Hawalina Hawalina
AGROLAND The Agricultural Sciences Journal (e-Journal) Vol 5 No 2 (2018): December
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/agroland.v5i2.320

Abstract

Kiwi fruit takes about 25 weeks from flower bloom until it reaches physiological maturity, so the time required to produce kiwi seeds from seeds in large quantities and uniform is very long. Tissue culture is one method that can be used to obtain a lot of kiwi seeds and uniforms with large quantities in a faster time. The purpose of this study was to examine various types of media compaction materials for the growth of kiwi shoots in vitro. This study was prepared based on Completely Randomized Design (RAL) with 5 treatments and repeated 4 times so that there were 20 experimental units, each experiment using 2 explants so that there are 40 eksplan. The treatments were: MA 1: Agar Swallow Globe 8 g / l, MA 2: Agar Swallow Globe 4 g / l + Agar Nutrijell 4 g / l, MA 3: Agar Swallow Globe 4 g / l + Agar Nutrijell 5 g / l, MA 4: Phytagel 2.2 g / l, MA 5: Agar Nutrijell 11 g / l. Observation variables are When shoots appear, Number of shoots, number of leaves, Number of Roots, number of root hair. The results showed Swallow Globe 4 g / l + Agar Nutrijell 4 g / l treatment gave the highest average number of shoots, the highest number of leaves and roots, this proved that the combination of Swallow Globe and Nutrijell agar gave a good condition for shoot growth kiwi plant.
Initiation of Grape (Vitis vinifera L.) on Different Sterilization Techniques Ratu Mentari Dewi; Ramal Yusuf; Sri Anjar Lasmini; Hawalina Hawalina
AGROLAND The Agricultural Sciences Journal (e-Journal) Vol 5 No 2 (2018): December
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/agroland.v5i2.323

Abstract

The most crucial step during the development of tissue culture is the method of explant sterilization. Especially, explants is sourced directly from fields that were more susceptible to microbial contaminations. This study aimed to obtain sterilization technique from several sterilant for reduced contamination and support growth of shoots grape in vitro. This research was conducted at the Laboratory of Plant Biotechnology, Faculty of Agriculture, University of Tadulako, during January to April 2018. The study used a factorial completely randomized design, one factor was tested i.e S1 = Taft 8,3 g/L (0,1% carbendazim), S2 = taft 25 g/L (0,3% carbendazim), S3 = taft 41,67 g/L (0,5% carbendazim), S4 = Taft 8,3 g/L (0,1% carbendazim) + HgCl2 0,1%, S5 = Taft 25 gr/L (0,3% carbendazim) + HgCl2 0,1% dan S6 = 41,67 gr/L (0,5% carbendazim) + HgCl2 0,1 %. There were six treatment and each treatment was replicated three times to obtain 18 experimental units. Each experimental unit using two explant. Data were analyzed using analysis of variance and followed by Honestly Significant Difference test at level of 5% if the treatment effects were significant. The results showed sterilization technique using taft 41,67 gram / L containing 0.5% carbendazim for 30 minutes, followed by 70% alcohol for 30 seconds and HgCl2 0,1% for 10 minutes gave free contamination for explant.whereas, Taft 8.3 g / L (Carb 0.1%) for 30 min, followed by 70% alcohol for 30 seconds is a sterilization technique that does not inhibit explant growth as indicated from the most callus formation.
Co-Authors Mahfudz Mahfudz Mardiana Mardiana Ramal Yusuf Ratu Mentari Dewi Sri Anjar Lasmini Zainuddin Zainuddin