INDONESIAN JOURNAL OF PHARMACY
Vol 30 No 3, 2019

Phytochemical analysis, antioxidant, antibacterial and antidiarrheal activities of Limnophila repens

Afroza Akter (Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.)
Mohammad Anwarul Basher (Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.)
Roni Roy (Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.)
Sharmin Sultana (Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.)
A F M Shahid Ud Daula (Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.)



Article Info

Publish Date
20 Sep 2019

Abstract

The study was carried out to determine the phytochemical content, antioxidant, antibacterial and antidiarrheal activity of methanolic extract of whole plant of Limnophila repens. The total phenolic, flavonoids, flavonols, tannins, β-carotene, lycopene, chlorophyll-α, and chlorophyll-β contents were found to be 65.21 ± 0.004 mg GAE/g , 44.83 ± 0.003 mg QE/g , 17.21 ± 0.002 mg QE/g, 56.27 ± 0.002 mg GAE/g , 1.32 ± 0.01 µg/g, 0.93 ± 0.012 µg/g, 11.85 ± 0.04 mg/g and 9.69 ± 0.037 mg/g, respectively. In vitro antioxidant potential of L. repens was assessed using various methods like by 2,2-diphenyl-1 picrylhydrazyl (DPPH), hydroxyl radical scavenging assay, reducing power assay, FRAP assay and thiobarbituric acid assay. IC50 values of the aforesaid tests were found to be 2.33 mg/ml, 442.31 µg/ml, 374 µg/ml, 55.09 µg/ml, and 4.30 mg/ml, respectively. The total antioxidant capacity was observed 140.7 ± 0.004 mg/g AAE. Aantibacterial activity of the methanolic extracts of whole plant of L. repens was evaluated using the disk diffusion method. The plant extract didn’t show any activity against Gram-positive (except Staphylococcus aureus) and Gram-negative bacteria. Finally, castor oil induced method was conducted to investigate the antidiarrheal activity of L. repens. At the dose of 400 mg/kg, the plant extract (35.62% inhibition of diarrhea) exhibited stronger effect compared to standard drug, loperamide (27.4% inhibition of diarrhea).

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Journal Info

Abbrev

3

Publisher

Subject

Medicine & Pharmacology

Description

Indonesian Journal of Pharmacy (ISSN-e: 2338-9486, ISSN-p: 2338-9427), formerly Majalah Farmasi Indonesia (ISSN: 0126-1037). The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas ...