INDONESIAN JOURNAL OF PHARMACY
Vol 30 No 3, 2019

The Application of Multiplate Resazurin Reduction Assay in The Screening for Anti-Mycobacterial Activity from Indonesian Medicinal Plants

Martha Sari (Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI))
Gita Syahputra (Laboratory for Applied Genetic Engineering and Protein Design, Biotechnology Research Center)
Wien Kusharyoto (Laboratory for Applied Genetic Engineering and Protein Design, Biotechnology Research Center)



Article Info

Publish Date
07 Aug 2019

Abstract

Tuberculosis (TB) is an airborne illness generated by Mycobacterium tuberculosis (Mtb), also one of the prominent infectious killers of adults worldwide. There is a pressing need to expand novel anti-mycobacterial drugs because of the increasing resistance of pathogenic mycobacteria to existing antibiotics. Native compounds acquired from microbial resources and medicinal cultivars have played an essential part as the origin of TB medications. The microplate resazurin reduction assay (MRRA) is generally utilized to assess natural and synthetic compounds for anti-mycobacterial activity. In our work, the MRRA method was employed to evaluate the anti-mycobacterial activity of extracts from curative plants using Mycobacterium smegmatis and Mycobacterium bovis BCG and to compare them to rifampicin as an anti-mycobacterial drug. The optimized MRRA utilized 2% aqueous DMSO and 62.5 μg/mL resazurin as an indicator compound in 5% aqueous Tween 80. The optimal incubation time for M. smegmatis was 24 hours, and for M. bovis BCG was 48 hours. The methanolic plant extracts were acquired from various Indonesian medicinal plants known to have anti-mycobacterial activity. The MRRA method using M. smegmatis or M. bovis BCG as anti-mycobacterial targets offers a distinct advantage such as low-cost, rapid, and safe screening for anti-mycobacterial activity in a middle to high-through-put-format.

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Journal Info

Abbrev

3

Publisher

Subject

Medicine & Pharmacology

Description

Indonesian Journal of Pharmacy (ISSN-e: 2338-9486, ISSN-p: 2338-9427), formerly Majalah Farmasi Indonesia (ISSN: 0126-1037). The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas ...