INDONESIAN JOURNAL OF PHARMACY
Vol 30 No 3, 2019

Antibacterial Compound from Aspergillus elegans SweF9 an Endophytic Fungus from Macroalgae Euchema sp.

Hani Mulyani (1. Pharmacy Faculty, Pancasila University,Jl. Raya Lenteng Agung Timur No.56-80, Srengseng Sawah, Jagakarsa, Jakarta 2. Indonesian Institute of Sciences (LIPI))
Rizna Triana Dewi (Indonesian Institute of Sciences (LIPI))
Chaidir Chaidir (Pharmacy Faculty, Pancasila University,Jl. Raya Lenteng Agung Timur No.56-80, Srengseng Sawah, Jagakarsa, Jakarta)



Article Info

Publish Date
20 Sep 2019

Abstract

The antibiotic resistance of bacterial pathogens has become a serious health concern and encouragement to search for novel and efficient antimicrobial metabolites. On the other hand, endophytic fungi have great potential as a natural source for antimicrobial agents. The objective of this study was to isolation antibacterial compound from endophytic fungi of A.elegans SweF9. The fungus was stationarily cultured at 30°C for 12 days in potato malt peptone (PMP) medium, then extracted with ethyl acetate. The antibacterial activities of the extract were evaluated by agar well diffusion method against Gram-poitive (Bacillus subtilis and  Staphylococcus aureus) and Gram-negative (Escherichia coli) bacterial strains. The broth extract was able to inhibit the growth of E. coli, S. aureus and B. subtilis with antibacterial activity index compared to streptomycin sulfate were 84.6%, 91.6%, and 90% respectively. The active compound (1) was purified to yield amorphous white and identified using FTIR, NMR, and EI-MS analyses, revealed identified as (+) - epi-Epoformin. The compound showed an antibacterial activity index of E. coli, S. aureus and B. subtilis  bacterial were 38%, 45%, and 47%, respectively.  Based on these results endophytic fungi A. elegans SweF9 can be used as a new source of potential antibacterial compounds

Copyrights © 2019






Journal Info

Abbrev

3

Publisher

Subject

Medicine & Pharmacology

Description

Indonesian Journal of Pharmacy (ISSN-e: 2338-9486, ISSN-p: 2338-9427), formerly Majalah Farmasi Indonesia (ISSN: 0126-1037). The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas ...