AGRIVITA, Journal of Agricultural Science
Vol 41, No 3 (2019)

Illegitimacy Testing of Elaeis guineensis Population Based on Simple Sequence Repeat Markers

Lalu Firman Budiman (PT. Astra Agro Lestari Tbk)
Ardha Apriyanto (PT. Astra Agro Lestari Tbk)
Adi Pancoro (School of Life Sciences & Technology, Institut Teknologi Bandung)
Sudarsono Sudarsono (PMB Lab., Department of Agronomy and Horticulture, Bogor Agricultural University)



Article Info

Publish Date
25 Oct 2019

Abstract

Illegitimacy is a factor negatively affecting controlled pollination in Elaeis guineensis breeding programs and it may happen in any step of hybridization processes, starting from early stages of parent selection and labeling to the last stage of the replicated field trial. Availability of method for testing the existence of illegitimacy among progenies of oil palm is beneficial. Four half-sib family populations consisted of 83 individuals were evaluated. Sixteen loci of SSR markers were utilized to genotype plant materials and identify illegitimate individuals. The legitimate parents and illegitimate progenies were evaluated using CERVUS and COLONY softwares. The results showed that the 16 SSR marker loci evaluated were having medium to high PIC values and they were both informative and suitable for parent-offspring analysis. The results also showed that the 16 SSR markers were sufficient for the illegitimacy testing using the COLONY software. Moreover, this study did not find any illegitimate individual among the four progeny populations. The generated SSR marker data were also successfully used to assign and to reconstruct the expected pedigree of the progenies. This can be used as an example of molecular marker utilization to improve the integrity of breeding program of oil palms.

Copyrights © 2019






Journal Info

Abbrev

AGRIVITA

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

AGRIVITA Journal of Agricultural Science is a peer-reviewed, scientific journal published by Faculty of Agriculture Universitas Brawijaya Indonesia in collaboration with Indonesian Agronomy Association (PERAGI). The aims of the journal are to publish and disseminate high quality, original research ...