HAYATI Journal of Biosciences
Vol. 17 No. 1 (2010): March 2010

Single Strand Conformation Polymorphism Method for Initial Detection DNA Sequences Homogeneity

Topik Hidayat (Indonesia University of Education)
Adi Pancoro (Bandung Institute of Technology)

Article Info

Publish Date
02 Mar 2010

Abstract

In molecular phylogenetic study, homogeneity of DNA sequences is a prerequisite before putting it into practice. Internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) has been common in phylogenetic study, but a homogenous sequence is often difficult to obtain. Here we use single-stranded conformation polymorphism (SSCP) method to detect homogeneity for nine pooled amplified products of ITS region. Our results suggested that SSCP method has been applicable in detection homogeneity of ITS region prior to using it in sequencing processes.

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Journal Info
HAYATI Journal of Biosciences
Website

Abbrev

hayati

Publisher

Institut Pertanian Bogor

Subject

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

Description

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, ...