Agroland : Jurnal Ilmu-Ilmu Pertanian
Vol 17, No 2 (2010)

DETEKSI BEGOMOVIRUS PADA TANAMAN CABAI MERAH DENGAN I-ELISA TEST DAN TEKNIK PCR

S. Mudmainah (Unknown)
Purwanto Purwanto (Unknown)



Article Info

Publish Date
26 Mar 2012

Abstract

The aim of this research was to study the symptom appear in the fields caused by Begomovirus or other viruses. The laboratory research was conducted using I-ELISA to comfirm the presence of Begomovirus and other mixed viruses and PCR to study coat protein band DNA of Begomovirus.  The sample was collected from the plant that showed sympton of Begomovirus infection.  The results of I-ELISA detection showed that the symptoms was varied in the field.  The symptoms were mixed infection among Begomovirus and Tobamovirus, Cucumovirus, Potyvirus. Based on PCR detection, using universal primer coat protein of Begomovirus could amplify DNA fragment at 580 bp

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Journal Info

Abbrev

AGROLAND

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Agroland : jurnal Ilmu-ilmu pertanian diterbitkan 3 kali dalam satu tahun. Jurnal ini diterbitkan oleh Fakultas pertanian Universitas Tadulako dengan tujuan untuk meningkatkan kapasitas Dosen diLingkup Fakultas Pertanian dalam Bidang Penelitian dan sebagai penunjang dalam Tri Dharma Perguruan ...