The aims of this study were to know the effect of addition of NaNO3 and K2HPO4 on BG-11 medium on biomass and chlorophyll concentration of Tetraselmis chuii and to fine out the best concentration of NaNO3 and K2HPO4 on BG-11 medium to produce highest biomass and chlorophyll concentration of Tetraselmis chuii. This study used a factorial randomized block design.The first factor was NaNO3consists of three levels i.e. 0.5, 1.5 and 2.5 g/l. The second factor wasK2HPO4consists of three levels i.e. 0.020, 0.035 and 0.050 g/l. The experiments were grouped into 2 groups based on the time of culturitation, in order to obtain 18 experimental units. Data were analyzed by analysis of variance and if the treatment significantly affected the parameter followed by Duncan test. The result showed that addition of NaNO3 and K2HPO4had very significant effect on the concentration of biomass and chlorophyll content of Tetraselmis chuii. The addition of NaNO31.5 g/l and K2HPO40.035 g/l was produce the highest of biomass and chlorophyll concentration of 3.3 x 106cells/ml and 10.17 mg/g, respectively. Keywords : biomass, chlorophyll, K2HPO4, NaNO3, Tetraselmis chuii.
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