MAKARA of Science Series
Vol 17, No 1 (2013): April

Identification of Gene Resistance to Avian Influenza Virus (Mx Gene) among Wild Waterbirds

Dewi Elfidasari (Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor, Bogor 16680, Indonesia)
Dedy Duryadi Solihin (Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor, Bogor 16680, Indonesia)
Retno Damayanti Soejoedono (Department Animal Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, Institut Pertanian Bogor, Bogor 16680, Indonesia)
Sri Murtini (Department Animal Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, Institut Pertanian Bogor, Bogor 16680, Indonesia)



Article Info

Publish Date
10 Jun 2013

Abstract

The Mx gene is an antiviral gene used to determine the resistance or the susceptibility to different types of viruses, including the Avian Influenza (AI) virus subtype H5N1. The AI virus subtype H5N1 infection in chickens causes Mx gene polymorphism. The Mx+ gene shows resistant to the AI virus subtype H5N1, whereas the Mx- gene shows signs of susceptible. The objective of this research was to detect the Mx gene in wild aquatic birds using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method with the primer pairs F2 and NE-R2/R and the RsaI restriction enzyme. DNA samples were obtained from eight species of wild waterbirds with positive and negative exposure to the AI virus subtype H5N1. DNA amplification results showed that the Mx gene in wild aquatic birds is found in a 100 bp fragment, which is the same as the Mx gene found in chickens. However, unlike chickens, the Mx gene in wild aquatic birds did not show any polymorphism. This study proves that Mx- based resistance to AI virus subtype H5N1 in different in wild birds than in chickens. Keywords: Mx gene, wild waterbirds, exposure, AI virus subtype H5N1, resistance Keywords: Mx gene, wild waterbirds, exposure, AI virus subtype H5N1, resistance

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Journal Info

Abbrev

science

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Materials Science & Nanotechnology Physics

Description

MAKARA Journal of Science publishes high quality research on fundamental science and provides a broad-based forum for the information sharing of ongoing research and development in science. Topics covered include physics, chemistry and biology. All submitted manuscripts will be subjected to the peer ...