Chem Info
Vol 1, No 1 (2013)

Isolasi, Identifikasi Serta Uji Aktivitas Antibakteri Senyawa Golongan Triterpenoid Dari Ekstrak Daun Binahong (Anredera cordifolia (Ten.) Steen) Terhadap Staphylococcus aureus dan Escherichia coli

Murdianto, Agus Ria (Unknown)

Article Info

Publish Date
20 Jan 2013

Abstract

Isolation, identification, and antibacterial activity examination of triterpenoid compounds from leaves of Binahong (Anredera cordifolia (Ten.) Steen). Maseration of 1 kg dry powder of those leaves using n-hexane respectively yielded 43,2 g. The n-hexane extracts obtained contained triterpenoids based on fitochemical test of Lieberman-Burchard. Compound separation of the n-hexane extract using column chromatography produced isolate which contained triterpenoids in fraction A. The isolate inhibited the growth of Staphylococcus aureus and Escherichia coli at Minimal Inhibition Concentration 100 ppm with weak inhibition zone. The results of identification using UV-Vis spectrophotometry showed a maximum absorption at a wavelength of 239 nm. Based on FT-IR spectrogram shows the groups, there were OH , C=C, C-C, C=O, -C-H, -CH3,-CH2, and C-O. LC-MS spectrogram indicated that the isolated had a molecular weight 562 g / mol of suspected compounds 2,3,19,23-tetrahydroxy-12-ene-24,28-dimethyl ester.

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Journal Info
Chem Info
Website

Abbrev

kimia

Publisher

Universitas Diponegoro

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

CHEMINFO JOURNAL adalah jurnal saintifik yang diterbitkan secara periodik 3 bulanan oleh Jurusan Kimia, Fakultas Sains dan Matematika Universitas Diponegoro Semarang. Jurnal ini sebagai media publikasi hasil karya ilmiah lulusan S1 Jurusan ...