Research Has done about activity of Fusarium oxysporum to hydrolysis water hyacinth (Eichhornia crassipes) with variation temperature. This research aimed to getting Fusarium oxysporum were adapted on water hyacinth (Eichhornia crassipes) fermentation media and it getting the data reducing sugar value of cellulolytic activity from Fusarium oxysporum that can hydrolysis of water hyacinth (Eichhornia crassipes) on the optimum temperature. Activity of Fusarium oxysporum based on the size of the level reducing sugars resulting from the hydrolysis of water hyacinth. The study was conducted in several step that is rejuvenating of Fusarium oxysporum to PDB (Potato Dextrose Broth), adaptation of  Fusarium oxysporum to fermentation media CMC (Carboxyl Methyle Cellulose), delignification (lignin removal from water hyacinth), adaptation of Fusarium oxysporum to water hyacinth fermentation media, creating a standard curve of glucose, fermentation of Fusarium oxysporum in water hyacinth fermentation media with variation in temperature, and testing activities Fusarium oxysporum to hydrolyze water hyacinth with variation in temperature. The result shows that Fusarium oxysporum can grow in fermentation media of water hyacinth and it can degrade water hyacinth to reduction sugar. The higher activity from Fusarium oxysporum to produce cellulose for hydrolysis cellulose of water hyacinth happened in optimum temperature 30°C that result reduction sugars 0,308 mg/mL from 0,25 gram dry water hyacinth.
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