AGROEKOTEKNOLOGI
Vol 3, No 1 (2015)

Analisis Keragaman Genetik Andaliman (Zanthoxylum acanthopodium DC.)Sumatera Utara Menggunakan Marka RAPD (Random Amplified Polymorphic DNA)

Ayu Oshin Yap Sinaga (Universitas Sumatera Utara)
Lollie Agustina P. Putri (Universitas Sumatera Utara)
Luthfi Aziz M. Siregar (Universitas Sumatera Utara)

Article Info

Publish Date
28 Jan 2015

Abstract

The aim of the research was to analyze the genetic diversity of the endemic andaliman inNorth Sumatera based on RAPD markers. A total of 30 accessions andaliman originated fromthe various region in North Sumatera, i.e.: Dairi, Karo Highland, Simalungun. The RAPDanalyse was used with 3 random primer: OPH-06, OPC-12 and OPN-03. These three primersshowed 32 bands, on which 31 were polymorphic (95.23%). Further genetic diversitycoefficient and the filogenetic dendogram were obtained using the Darwin 5.05 software. Theresults showed that 30 accessions of andaliman were clustered in three groups. Each groupconsisted of the accessions of three regions from different altitudes. There were 30 accessionsof three regions studied showed a high genetic diversity.Keywords: Andaliman, genetic diversity, random amplified polymorphic DNA

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Journal Info
AGROEKOTEKNOLOGI
Website

Abbrev

agroekoteknologi

Publisher

Universitas Sumatera Utara

Subject

Agriculture, Biological Sciences & Forestry

Description

Jurnal AGROEKOTEKNOLOGI merupakan jurnal ilmiah yang diterbitkan secara online berisikan hasil-hasil penelitian tentang hama dan penyakit tumbuhan, agronomi, pemuliaan tanaman dan ilmu ...