Jurnal Matematika Sains dan Teknologi
Vol. 10 No. 1 (2009)

ISOLASI DAN IDENTIFIKASI BAKTERI PEREDUKSI SULFAT PADA AREA PERTAMBANGAN BATU BARA MUARA ENIM, SUMATERA SELATAN

Muchamad Yusron (Institut Pertanian Bogor)
Bibiana W Lay (Institut Pertanian Bogor)
Anas M Fauzi (Institut Pertanian Bogor)
Dwi Andreas Santosa (Institut Pertanian Bogor)



Article Info

Publish Date
15 Aug 2009

Abstract

Sulfate reducing bacteria utilize sulfate as their terminal electron acceptor and reduce it to sulphide. Acid mine drainage, by-products of mining activities, is an acidic sulfate-rich wastewater suitable habitat for sulfate reducing bacteria. Isolation and identification of sulfate reducing bacteria collected from Muara Enim coal mining, South Sumatra was carried out at Laboratory of Environmental Biotechnology, Indonesian Center for Biodiversity and Biotechnology (ICBB), Bogor, and Laboratory of Microbiology, Faculty of Veterinary, Bogor Agricultural University. Postgate B liquid media was used for isolation and purification via serial dilution. Physiological and biochemical characterization was done based on Bergeys Manual of Determinative Bacteriology. Fifteen pure isolates have been isolated with diverse characteristics. Eight isolates can sustain at pH 3, while the rest sustain at pH 4 or above. Sulfate reduction efficiency of each isolates were different, but increased as the pH increased. The bacteria are classified as Desulfovibrio sp., which is characterized straight rods, motile, non spore-forming and able to grow in simple organic carbon.

Copyrights © 2009






Journal Info

Abbrev

JMST

Publisher

Subject

Agriculture, Biological Sciences & Forestry Mathematics Other

Description

Merupakan media informasi dan komunikasi para praktisi, peneliti, dan akademisi yang berkecimpung dan menaruh minat serta perhatian pada pengembangan Matematika, ilmu pengetahuan dan teknologi. Diterbitkan oleh Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas ...