Medical Laboratory Technology Journal
Vol 6, No 1 (2020): June

The Effect of Dehydroepiandrosterone on Ovarian Reserve in Ovarian Damage Caused by Methotrexate

Ali Doğukan Anğın (University of Health Sciences,)
Ebru Çöğendez (University of Health Sciences,)
İsmet Gün (University of Health Sciences,)
Önder Sakin (University of Health Sciences,)
Muzaffer Seyhan Çıkman (University of Health Sciences,)
Kayhan Başak (University of Health Sciences,)
Asuman Orçun Kaptanağası (University of Health Sciences,)
Yasemin Alan (İzmir Metropolitan Municipality Eşrefpaşa Hospital)
Murat Alan (University of Health Sciences)
Emrah Beyan (University of Health Sciences)

Article Info

Publish Date
22 May 2020


To determine the possible detrimental effects of multiple methotrexate doses has on the ovarian reserve and to determine the beneficial effects of dehydroepiandrosterone supplementation. The rats (n:24) divided into three groups; Group 1: control group, Group 2: dehydroepiandrosterone and methotrexate group (6mg/kg dehydroepiandrosterone dissolved in 0.1 ml sesame seed oil subcutaneously for ten days and 1mg/kg intramuscular methotrexate at the 1st, 3rd, 5th and 7th days) and Group 3: methotrexate group (1mg/kg intramuscular methotrexate at the 1st, 3rd, 5th and 7th day). The groups compared in regards to their histopathological ovarian damage scores and AMH values. It established that multiple methotrexate applications had a considerable effect on reducing vascular congestion in the ovarian tissue. Both in groups 2 and 3 AMH values found to be significantly lower. When this decline in the ovarian reserve examined comparatively; while both the group 2 and 3 reported having a considerable and continuous reduction in the AMH levels correlative to the control group; the primordial, primary and total follicle counts shown to stay statistically the same in the group 2 (p

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Journal Info





Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health


The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have been achieved in the area of medical laboratory sciences. This Journal particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It ...