<![CDATA[ABSTRACT Retinal neovascularization is the main problem that causes blindness in many types of eye desease. Neovascularization occurs in ischemic retina, and several important mediators that induce neovascularization is secreted by the surrounding cell and endothelial cell vessels. Matrix metalloproteinase-9 is an enzyme secreted by endothelial cell vessels and plays an important role in the pathogenesis of retinal induce neovascularization. Vascular endothelial cells were cultured and transfected with Matrix Metalloproteinase -9 RNA interference in two doses (100nM or 200nM) and the cells were taken at 24, 48 and 72 hours. The expression of Matrix Metalloproteinase -9 protein levels were examined by immunocytochemistry, and compared with control group. The data were analyzed with factorial anova. Statistical analysis showed that transfection with different doses of MMP-9 RNAi gave a significant different effect on MMP-9 expression (p-value < 0.05), while exposure time did not give significant difference (p-value < 0.05). This in-vitro study indicated that transfection of MMP-9 RNAi with dose 200nM reduced the expression of MMP-9 by 50% compared to the control. Because exposure time was not significant, the lowest expression of MMP-9 can be achieved by transfecting MMP-9 RNAi 200nM for 24 hours. This is the first study that demonstrates the effectiveness of RNA interference mediated targeting of MMP-9 to reduce the MMP-9 expression in vascular endothelial cell lines Key Words : Matrix Metalloproteinase -9 - RNA interference - vascular endothelial cell lines.]]>
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