JURNAL BIOLOGI INDONESIA
Vol 7, No 1 (2011): JURNAL BIOLOGI INDONESIA

SINTESIS ALKIL N-ASETILGLUKOSAMINA (ALKIL-GLCNAC) DENGAN ENZIM N-ASETILHEKSOSAMINIDASE YANG DIISOLASI DARI ASPERGILLUS SP. 501

Saskiawan, Iwan (Unknown)
Handayani, Rini (Unknown)

Article Info

Publish Date
12 May 2017

Abstract

ABSTRACTEnzymatic Synthesis of Alkyl N-acetylglucosamine (Alkyl-GlcNAc) by Application ofTransglycosylation Activity of ?-N-Acetylhexosaminidase from Aspergillus sp. 501. ?-NAcetylhexosaminidasefrom Aspergillus sp. 501 has transglycosylation and hydrolytic activity.Transglycosylation occur through transfer of N-acetylglucosamine (GlcNAc) which washydrolyzed from p-nitrophenyl-?-N-acetylglucosamine (pNP-GlcNAc) to various alcohols. Thetransglycosylation product was determined by Thin Layer Chromatography and HighPerformance Liquid Chromatography. Using this transglycosylation activity the novelcompound of Alkyl-GlcNAc was synthesized using N-acetylchitotriose as a donor and anethanol as an acceptor.Keywords: Alkyl N-acetylglucosamine, Transglycosylation, ?-N-Acetylhexosaminidase,Aspergillus sp. 501

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Journal Info
JURNAL BIOLOGI INDONESIA
Website

Abbrev

jurnal_biologi_indonesia

Publisher

Lembaga Ilmu Pengetahuan Indonesia

Subject

Biochemistry, Genetics & Molecular Biology

Description

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