Unesa Journal of Chemistry
Vol 3, No 3 (2014): Volume 3, Number 3, 2014

AKTIVITAS BAKTERI PROTEOLITIK YANG DIISOLASI DARI SUMBER AIR PANASSINGGAHAN, TUBANPROTEOLYTIC MICROORGANISMS ACTIVITY ISOLATD FROM HOT SPRINGSINGGAHAN, TUBAN

NUR RUSDWITASARI, YENI (Unknown)



Article Info

Publish Date
01 Jan 1970

Abstract

Abstrak. Penelitian ini bertujuan untuk mendapatkan bakteri penghasil protease termostabil dari sumber airpanas Singgahan, Tuban (Jawa Timur) serta menentukan aktivitas protease isolat. Isolasi bakteri dilakukanmenggunakan media Luria Bertani (LB). Penapisan dilakukan menggunakan media skim milk agar.Pengujian aktivitas proteolitik secara kuantitatif dilakukan dengan metode kolorimetri menggunakan reagenFolin Ciocalteu. Diantara 75 isolat yang diperoleh dari hasil isolasi, 27 isolat bakteri mampu tumbuh padamedia skim milk agar pada suhu inkubasi 53?C dan memiliki potensi sebagai penghasil protease denganindeks proteolitik (IP) 1.8-9. Isolat dengan nilai indeks proteolitik relatif besar kemudian diuji aktivitasproteolitik secara kuantitatif dengan metode kolorimetri. Aktivitas protease kasar tertinggi dimiliki oleh ST-30 yaitu sebesar 0.2520 U/mL dengan nilai IP 9 serta mampu bertahan sampai suhu 75?C.Kata-kata kunci: Bakteri termofilik, indeks proteolitik, aktivitas proteolitikAbstract. This research aim to got thermostable bacteria producing protease from hot spring Singgahan,Tuban (Jawa Timur) and determined the protease activity. Isolation was carried out using Luria Bertany(LB) medium while the screening using skim milk agar medium. Proteolytic activity quantitatively done withcolorimetric method using Folin Ciocalteau. Total of 75 strains of thermophilic bacteria were isolated and27 isolate bacteria grew well at skim milk agar medium in high temperature (53?C) and have potential asprotease producing bacteria with proteolytic index (PI) of 1.8-9. Isolate with high relative proteolytic indextested with colorimetric method. Crude protease ST-30 had the highest activity that was 0.2520 U/mL, PI 9and had ability to endure temperature until 75?C.Keywords : Thermophile bacteria, proteolytic index, proteolytic activity

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Journal Info

Abbrev

unesa-journal-of-chemistry

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

UNESA Journal of Chemistry is published online by the Department of Chemistry, Faculty of Mathematics and Natural Sciences, Surabaya State University, located at Jl Ketintang, Surabaya, East Java, Indonesia. 60231. Published 3 times a year (January, May and September) Aims and Scope: UNESA Journal ...