Unesa Journal of Chemistry
Vol 5, No 3 (2016): Volume 5, Nomer 3, 2016

VARIASI ETANOL-ASETONITRIL PADA PEMURNIAN N-ASETILGLUKOSAMIN HASIL DEGRADASI ENZIMATIS KITIN JENIS AMORF

DEWI NURROHMAWATI, FITRIA (Unknown)



Article Info

Publish Date
28 Nov 2016

Abstract

Abstrak. Telah dilakukan penelitian yang bertujuan untuk memurnikan N-asetilglukosamin serta mengetahui persen rendemen yang dihasilkan setelah proses pemurnian. Pemurnian N-asetilglukosamin dilakukan dengan teknik pemurnian bertingkat dengan metode pengendapan menggunakan etanol dan asetonitril. Persen rendemen dihitung dengan membandingkan jumlah N-asetilglukosamin yang terbentuk terhadap kitin amorf mula-mula. Hasil pemurnian menunjukkan bahwa N-asetilglukosamin dapat dimurnikan dengan pemurnian bertingkat menggunakan etanol dan asetonitril menghasilkan kromatogram seperti standar. Rendemen N-asetilgukosamin yang dihasilkan setelah proses pemurnian yaitu sebesar 10,52%.   Kata Kunci: N-asetilglukosamin, pemurnian, persen rendemen   Abstract. Research has been done  that aims to purify N-acetylglucosamine and knowing percent rendemen produced after the purification process. N-acetylglucosamine purification was performed using multilevel purification by precipitation method using ethanol and acetonitrile. Percent rendemen is calculated by comparing the number of N-acetylglucosamine formed on the first amorphous chitin. The results showed that the purification of N-acetylglucosamine can be purified by purification using ethanol and acetonitrile graded with the standard approach resulting chromatogram. Percent rendemen produced after the purification process that is 10.52%.   Keywords: N-acetylglucosamine, purification, percent rendemen

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Journal Info

Abbrev

unesa-journal-of-chemistry

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

UNESA Journal of Chemistry is published online by the Department of Chemistry, Faculty of Mathematics and Natural Sciences, Surabaya State University, located at Jl Ketintang, Surabaya, East Java, Indonesia. 60231. Published 3 times a year (January, May and September) Aims and Scope: UNESA Journal ...