Jurnal Ilmiah Manuntung
Vol 6 No 1 (2020): Jurnal Ilmiah Manuntung

PENETAPAN KADAR TOTAL FLAVONOID EKSTRAK ETANOL KACANG MERAH (Phaseolus vulgaris L.) SECARA SPEKTROFOTOMETRI VISIBEL

Lindawati, Novena Yety (Unknown)
Ma'ruf, Sabilla Hudzaifah (Unknown)



Article Info

Publish Date
30 Jun 2020

Abstract

Red kidney bean is one of the string beans that contain highest-fiber with a concentration 26.3 gram/100 gram substance. Red kidney bean contains flavonoid tannin steroid/triterpenoid and coumarin. The Flavonoid in red kidney bean has anti-diabetes activity. This research aims to determine total flavonoid content in the red kidney bean extract. Extraction is done using the maceration method with 70% ethanol solvent. The extract which is got is used for qualitative and quantitative analysis. Quercetin used as a standard solution. The qualitative analysis of flavonoid using TLC, Wilstater Cyanidin by forming a red complex and NaOH by a forming yellow complex. The Quantitative analysis was using colorimetry method with reagent AlCl3 to form complexes between AlCl3 and keto groups. Quantitative analysis continued with Visible Spectrophotometry on a wavelength of 430.0 nm and operating time at the 27th minute. The results of the qualitative test showed that the extract was positive flavonoids. The average concentrations of total flavonoids were 0,4733% extract with a variation coefficient value of 1,2%.

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Journal Info

Abbrev

jim_akfarsam

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Jurnal Media Pertanian dipublikasikan dalam Bahasa Indonesia dan diterbitkan dua kali dalam setahun. Jurnal ini mempublikasikan artikel hasil penelitian dan artikel review bidang ilmu Agronomi secara luas. Semua tulisan yang masuk hendaknya bukanlah hasil plagiat dan belum dipublikasikan. Artikel ...