Journal of Health Sciences and Medicine
Vol 2 No 1 (2018): JHSM (February 2018)

Identification of Active Antioxidant Compounds from Ungu Leaf Ethanol Extract (Graptophyllum pictum L. Griff)

Ni Luh Rustini (Universitas Udayana)



Article Info

Publish Date
02 Feb 2018

Abstract

This study was conducted to determine the chemical compounds contained in ungu leaf ethanol extract that has antioxidant properties. Ungu leaf powder (2000 g) was extracted with 10,000 mL of 96% ethanol yielding 51.77 g thick purple-green viscous extract. The result of phytochemical test on ungu leaf ethanol extract showed that the extract contained flavonoid, saponin, phenolic and steroid, with the highest content being steroid. The separation of the ethanol extract was carried out by column chromatography using silica gel as a stationary phase and a mixture of n-hexane: dichloromethane: ethyl acetate (2: 1: 1) as the mobile phase, yielding 10 fraction groups. Fraction F1 is relatively pure, then identified with UV-Vis, IR, and GCMS spectrophotometers. The results of identification with UV-Vis, IR, and GC-MS spectrophotometers showed that ungu leaf ethanol extract containing antioxidant activity contained stigmasterol compounds, namely: stigmasta-5,22-dien-3-ol and stigmast acid-5-en-3-ol.

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Journal Info

Abbrev

JHSM

Publisher

Subject

Medicine & Pharmacology

Description

Journal of Health Sciences and Medicine is an Indexed peer-reviewed open access journal which publishes original scientific articles, reviews and short communications that are not under consideration for publication elsewhere. The journal publishes papers based on original research that are judged ...