Indonesian Applied Physics Letters
Vol. 1 No. 1 (2020): June

Design Of Autofocus Microscope With Histogram Method For Tuberculosis Bacteria Observation

Mohammad Kholil (Universitas Airlangga)
Riries Rulaningtyas (Universitas Airlangga)
Winarno Winarno (Universitas Airlangga)

Article Info

Publish Date
11 Aug 2020

Abstract

This research was conducted to design an autofocus microscope with a histogram method that can observe Tuberculosis (TB) bacteria. The bacteria observed were preparations or phlegm preparations which had been stained with Ziehl Neelsen. The microscope is designed to be equipped with a program to control the focus motor that moves the microscope tube and the program to digitally display the image and histogram of TB bacteria. Histograms are analyzed based on intensity values spread between 0-255 and the entropy value is sought. The measurement results that have been carried out as many as 20 times the field of view of the TB bacteria show that the most focused areas have the highest entropy value with an accuracy level ranging from 81.90476% to 100% at 1000 times the magnification.

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Journal Info
Indonesian Applied Physics Letters
Website

Abbrev

IAPL

Publisher

Universitas Airlangga

Subject

Chemical Engineering, Chemistry & Bioengineering Electrical & Electronics Engineering Materials Science & Nanotechnology Physics

Description

Indonesian Applied Physics Letter is an multi-disciplinary international journal which publishes high quality scientific and engineering papers on all aspects of research in the area of applied physics and wide practical application of achieved results. The field of IAPL, which can be described as ...