<![CDATA[Spoiled by-catch fish (HTS) has a high protein content, so that is potential to be used as peptone raw material. This research aimed to produce peptone from spoiled by-catch fish and to apply it as nutrient source of bacteria and yeast growth. Peptone was produced by the addition of 0.3% papain enzyme 5 hours hydrolysed process. The result showed that peptone from by-catch fish had protein of 71.39%, solubility of 99.96%, nitrogen of 11.42%, α-Amino free nitrogen of 1.76%, AN/TN ratio of 15.41%, salt of 7.82% and pH of 7.1. The results ofthe analysis of the specific growth rate (μ max) and the average growth rate indicated that the media containing peptone HTS did not give a better growth effect on Staphylococcus aureus and Sacharomyces cereviceae than that grown on media without peptone and commercial peptone. Escherichiacoli grown in media containing peptone HTS was not consumable due to the lower values of μ max and the average growth rate.Keywords: by-catch fish, growth nutrient, hydrolysis, peptone]]>
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