Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Vol 16, No 1 (2021): May 2021

Heat Resistance of Probiotic Candidate Enterococcus faecalis R22B in Different Matrices

Shanti Dwita Lestari (Sriwijaya University)
Rinto Rinto (Program Studi Teknologi Hasil Perikanan Universitas Sriwijaya, Jl. Raya Palembang-Prabumulih Km. 32 Indralaya, Ogan Ilir 30662, Indonesia)
Indah S Wahyuni (Program Studi Teknologi Hasil Perikanan Universitas Sriwijaya, Jl. Raya Palembang-Prabumulih Km. 32 Indralaya, Ogan Ilir 30662, Indonesia)
Sherly Ridhowati (Program Studi Teknologi Hasil Perikanan Universitas Sriwijaya, Jl. Raya Palembang-Prabumulih Km. 32 Indralaya, Ogan Ilir 30662, Indonesia)
Wulandari Wulandari (Program Studi Teknologi Hasil Perikanan Universitas Sriwijaya, Jl. Raya Palembang-Prabumulih Km. 32 Indralaya, Ogan Ilir 30662, Indonesia)



Article Info

Publish Date
24 May 2021

Abstract

Fermented fish, including rusip and bekasam, contains lactic acid bacteria, some of which have probiotic properties. Considering the beneficial effect, the count of viable cells in probiotic carrier foods must be at least 106 CFU/g throughout the product shelf-life. The processing and storage condition significantly affect the viability of probiotics in carrier foods. This research was conducted to determine the heat resistance of probiotic candidate Enterococcus faecalis R22B isolated from rusip in three different media: bekasam, rusip, and physiological saline solution (NaCl 0.85%). E. faecalis R22B was heated in each medium at a temperature of 70 °C for 0, 2, 4, 8, and 12 min. The media characteristics showed differences within the parameters included pH values of 4.41-6.99; aw 0.67-0.92; moisture content 72.56-98.09%; ash content 0.69-12.32%; protein content 0-14.7%; fat content 0-0.84%; and carbohydrate content from 0-4.93%. The results showed that the difference in the heating medium had no significant effect (p0.05) on the heat resistance of the bacteria, as indicated by the D70 value. The required average heating time at 70 oC to decrease E. faecalis R22B cells by 1log10 (D70 value) in bekasam, rusip, and physiological saline media were 3.17, 3.29, and 3.55 min, respectively. Despite having a lower DT-value, the reduction of bacteria cells in three media heated at 70 oC showed no difference (p0.05); therefore, bekasam and rusip are suitable for probiotic carrier food.

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Journal Info

Abbrev

squalen

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

Description

Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be ...