Background: Laboratory examination has several factors that can affect the results of the examination, one of which is the pre-analytic factor that can affect the results of erythrocyte examination is the ratio between blood volume and anticoagulant. If the blood volume is insufficient, the anticoagulant causes red blood cells to become krenated, and if the excess blood volume can cause anticoagulants it can cause blood clots. Research Objective: This study aims to determine the ratio of the number of erythrocytes in the blood sample volume of 3 mL, 2 mL, and 1 mL with anticoagulant K2EDTA. Research Methods: This study used primary data with a hematological examination at the UTD RSUD Dr. H. Abdul Moeloek Bandar Lampung. This type of research is quantitative using an observational analytic design with a crossapproach sectional through a hematological examination using the Hematology Alayzer Mindray BC-3600 with a sample size of 40 respondents who meet the inclusion and exclusion criteria. Results: The results of the mean examination of the number of erythrocytes between the blood volume of 1 mL, 2 mL, 3 mL with the anticoagulant K2EDTA had different results, at a volume of 3 mL showed the lowest results. Conclusion: There is no significant difference between the examination of the number of erythrocytes with the blood sample volume of 1 mL, 2 mL, and 3 mL in thetube vacutainer K2EDTA Keywords: Hematology Examinatio;, Blood Volume; K2EDTA.
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