Biota
Vol 14 No 1 (2021)

Antimicrobial Resistance and Molecular Identification of Clinical Multi-Drug Resistant Enterobacter Cloacae

Siti Nur Fajriah (Sekolah Tinggi Ilmu Kesehatan Mitra Keluarga)
Maulin Inggraini (Sekolah Tinggi Ilmu Kesehatan Mitra Keluarga)
Noor Andryan Ilsan (Sekolah Tinggi Ilmu Kesehatan Mitra Keluarga)
Reza Anindita (Unknown)



Article Info

Publish Date
30 Jun 2021

Abstract

Enterobacter cloacae is a Gram-negative bacteria causing nosocomial infections. This bacteria has increased resistance to various antibiotics in the past five years, resulting in a multi-drug-resistant (MDR) phenotype. In particular, MDR E. cloacae causes longer hospitalization time, increases medical costs, and affects morbidity and mortality. This study aimed to observe the minimum inhibitory concentration (MIC) of clinical E. cloacae towards several antibiotics and molecular identification of MDR E.cloacae. This study was conducted in a descriptive design. Secondary data was collected at the microbiology laboratory of the Teaching Hospital in Bekasi, Indonesia, from May to September 2020. Sampel was carbapenem resistant E.cloacae. The isolate was originated from a human clinical specimen, then was confirmed molecular identification using 16s rRNA. In this study, only one carbapenem-resistant E. cloacae, which is also MDR bacteria, was found. This E. cloacae was categorized as MDR bacteria since it was resistant to more than three antibiotic classes, including carbanemen, extended-spectrum cephalosporin, penicillins + β lactamase inhibitor, antipseudomonal penicillins + β lactamase inhibitor aminoglycoside, and penicillin. Vitek 2 identification of this isolate was E. cloacae complex. It showed similar results to molecular identification based on a partial sequence of 16s rRNA. BLASTn result of the trimmed sequence was E. cloacae with 99.78 % similarity.

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Journal Info

Abbrev

jb

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Medicine & Pharmacology

Description

Focus and Scope of Biota on Bioscience, includes: cell biology and genetics, biodiversity, ecology, physiology and biotechnology. Biota covers all life forms, including microbes, fungi, plants, virus, animal and human. Focus and Scope of Biota on Biology Education, includes: Experimental Research, ...