Cassowary
Vol 4 No 1 (2021): Januari

Keragaman genetik Anggrek Grammatophyllum scriptum asal biji dari hasil Kultur In Vitro berdasarkan penanda RAPD

Zarima Wibawati (Program Studi S2 Ilmu Pertanian, Program Pascasarjana, Universitas Papua Jalan Gunung Salju, Amban, Manokwari, Papua Barat, 98314, Indonesia)
Amelia Sarungallo (Program Pascasarjana, Universitas Papua Jalan Gunung Salju, Amban, Manokwari, Papua Barat, 98314, Indonesia)
Barahima Abbas (Program Pascasarjana, Universitas Papua Jalan Gunung Salju, Amban, Manokwari, Papua Barat, 98314, Indonesia)

Article Info

Publish Date
30 Jan 2021

Abstract

Propagation through tissue culture by using orchid seed as explants will produce a lot of orchid plants. This study aims to measure the genetic character of orchid plantlets that were regenerated from seeds which have been resulted from in vitro culture. The genetic character of the original orchid plants produced from in vitro culture was determined using Random Amplified Polymorphic DNA (RAPD) molecular markers. The results showed that the primers used in the RAPD analysis showed a polymorphic band pattern of 14 DNA bands, with sizes between 500 bp - 8000 bp. The genetic distance of Grammatophyllum scriptum orchids that was regenerated from seeds is between 0.229 and 0.649. The progenis produced from in vitro culture were clustered into seven groups at a dissimilarity coefficient of 45%.

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Journal Info
Cassowary
Website

Abbrev

cs

Publisher

Universitas Papua

Subject

Agriculture, Biological Sciences & Forestry Environmental Science

Description

Cassowary is a Scientific Journal of Management of Natural Resources and Environment, aims to disseminate research findings on environmental and natural resource management. The writings can be published in this journal can be shaped dissertations, theses, research reports, scientific papers and ...