Cassowary
Vol 4 No 1 (2021): Januari

Keragaman Morfologi dan Genetik Beberapa Aksesi Tanaman Sagu (Metroxylon Sagu Rottb.) Berdasarkan Penanda Molekuler Gen Mat-K

Eka Fitri Wulandari (Program Studi Agroteknologi, Fakultas Pertanian, Universitas Papua, Jalan Gunung Salju, Amban, Manokwari, Papua Barat, 98314, Indonesia)
Nouke Lenda Mawikere (Program Studi S2 Ilmu Pertanian, Program Pascasarjana, Universitas Papua Jalan Gunung Salju, Amban, Manokwari, Papua Barat, 98314, Indonesia)
Barahima Abbas (Program Pascasarjana, Universitas Papua Jalan Gunung Salju, Amban, Manokwari, Papua Barat, 98314, Indonesia)



Article Info

Publish Date
30 Jan 2021

Abstract

Sago palm (Metroxylon sago Rottb.) is one of the carbohydrate-producing crops that has great potential in supporting food security programs. This study aims to determine the morphological and genetic diversity based on the mat-K gene marker of 15 accessions of sago pal that have been collected by the Sago Research Consortium. This research was conducted by preparing plant samples, extracting DNA, amplifying DNA with a PCR tools, visualizing the results of DNA amplification, sequencing, editing DNA sequencing, and blasting. The results showed that the sago palm accessions collected by the UNIPA Sago Research Consortium were morphologically different in the Russet stages. Based on the maturase K (mat-K) gene marker of 15 sago palm accessions were divided into two haplotypes, namely haplotype 1 which experienced deletion at base number 5 (amino acid phenylalanine) and haplotype 2 which did not experience deletion so it had phenylalanine amino acid. Phylogenetic analysis showed that 15 sago palm accessions were divided into 2 groups, namely group 1 and group 2. Sago palm were observed closely related to Metroxylon warburgii with genetic distance of 0.001.

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Journal Info

Abbrev

cs

Publisher

Subject

Agriculture, Biological Sciences & Forestry Environmental Science

Description

Cassowary is a Scientific Journal of Management of Natural Resources and Environment, aims to disseminate research findings on environmental and natural resource management. The writings can be published in this journal can be shaped dissertations, theses, research reports, scientific papers and ...