International Journal of Applied Biology
Vol. 5 No. (1) (2021): International Journal of Applied Biology

Sperm Fertility of SK Kedu Chicken in Lactated Ringer’s-Egg Yolk Extender with 10% of DMSO

Sipora Petronela Telnoni Nella (Universitas San Pedro)



Article Info

Publish Date
06 Jul 2021

Abstract

This study was conducted to evaluate fertility of SK Kedu chicken post inseminated by frozen semen using lactated ringer’s-egg yolk with 10% of DMSO. Frozen semen of lactated ringer’s-egg yolk with 10% of DMSO was picked from container of Liquid Nitrogen (-196 ºC) and thawed in warm water at 37 ºC for 30 second. Frozen semen inseminated to 3 arab hens: A1, A2 and A3 with 100 motile sperm cell in 0.25 mL-1 using 2 straws and deposited into reproductive tract of hen as long as 7 cm in intra utein using AI gun. Egg was collected in day 2 after AI till day 14, labelled and incubated. Sperm fertility was estimated by using % sperm fertility formulation = fertile eggs / incubated eggs x 100, regardless of whether the eggs will hatch or not. Egg fertility are checked on 5 day after incubation. This result showed % sperm fertility of SK kedu chicken were A1 (77.78 %), A2 (33.33 %), and A3 (83.33 %). The mean of % sperm fertility was 72.22 %. It can be concluded that lactated ringer’s-egg yolk with 10% of DMSO can maintain sperm fertility of SK kedu chicken.

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Journal Info

Abbrev

ijoab

Publisher

Subject

Agriculture, Biological Sciences & Forestry Automotive Engineering Energy Environmental Science

Description

International Journal of Applied Biology is an international, peer reviewed journal, publishing high-quality research. International Journal of Applied Biology only publishes manuscripts in English. Please see the journal's Focus & Scope for information about its Scope and peer-review policy. ...