Omni-Akuatika
Vol 17, No 1 (2021): Omni-Akuatika May

Viability Test of Hydrocarbonoclastic Bacteria Consortium Entrapted on Sawdust Material

Devia Hartono Puteri (Raja Ali Haji Maritime University)
Aditya Hikmat Nugraha (Marine Science Department, Raja Ali Haji Maritime University)
Yeti Darmayati (Marine Microbiology Laboratory, Research Center for Oceanography, Indonesian Institute of Sciences)
Agung Dhamar Syakti (Environmental Science Department, Raja Ali Haji Maritime University)



Article Info

Publish Date
12 Aug 2021

Abstract

Oil spills in the sea are generally prevented in various ways, one of them is the bioaugmentation method through bacterial entrapment on sawdust carriers. The entrapment of bacteria is one of the bacterial immobilization techniques. The potential of bacterial consortium on sawdust is still not widely used, especially in long-term storage. The purpose of this study was to obtain the viability of hydrocarbonoclastic bacterial consortium on sawdust after 0, 7, and 41 days of storage. The bacterial consortium consists of Bacillus aquimaris, B. megaterium, B. pumilus, Halobacillus trueperi, and Rhodobacteraceae bacterium. The samples were tested by culturing the immobilized bacterial consortium of 0, 7, and 41 days on falcon tubes containing crude oil and seawater. The viability of the immobilized bacterial consortium was tested for 28 days (days 0, 7, 14, 21, and 28) by enriched the bacterial populations periodically. The viability test was also supported by the measurement of environmental parameters on the 1st day and the 28th day such as DO, pH, salinity and, temperature. The results show that bacterial consortium in all storage periods was viable on sawdust carriers which related to the high number of bacterial populations on the 28th day. The most viable bacterial consortium on sawdust was the 0-day storage (9.59 x 108 CFU/ml) which was indicated by the increases phase at the end of the day. It was proved that the bacteria are still productive and could degrade petroleum hydrocarbons.

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Journal Info

Abbrev

joa

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

Description

OmniAquatika is a scholarly refereed research journal which aims to promote the theory and practice, innovation, engineering and management as well as social-economic relevant in fisheries and marine sciences fields. The main aspects of research areas include, but are not exclusive to the current ...